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The map label for this gene is cytR [H]

Identifier: 126176479

GI number: 126176479

Start: 5028675

End: 5029706

Strand: Reverse

Name: cytR [H]

Synonym: Sbal_4294

Alternate gene names: 126176479

Gene position: 5029706-5028675 (Counterclockwise)

Preceding gene: 126176480

Following gene: 126176477

Centisome position: 98.1

GC content: 49.22

Gene sequence:

>1032_bases
ATGACAACAACAAAACACTGGACCCTAAAGAGCATAGCCAACGAGTTGGGTGTCTCTAATGCCACTGTTTCTAACGCGTT
TAATCGCCCCGACCAATTATCGGAAAAACGTCGTAACGACATTTTAGCTGCCTGCACTAAGTTGGGATATTTCGGGCCAA
ATAAAGCGGCTCAATCCTTACGTAAGGGCAAGTTCGATACTGTCGCCTTGGTATTGTCGGACAGCGTGGAATACATGGTG
TCCGATCCCGTCGCCAGTAAATTTATGAAGGGCGTGGCCTCTGTCTTGGAGCAAGAAAAGCTCAATTTGCTGCTGTTTTC
GGGCAGCTCAGACAGCGTGAATGCGGTGGCCGACTTTGTGGATGGTTTTATTTGTTATGGTCGCCCGCGCAATGCCCTGT
TAGCCGAGCAGTTAAAGCAGGTTAAGAAAAAGGTCGTCACGGTCGATTTTGATATCCACCGCAATGCATCTGTGAGCATA
GACAATAAGTTAGCTGCCTATGAAGTAGCGAAACTGGCATTGCAGTCGCCCGCGGACAATGTGGCCATTTTGGGGCTACG
TTTGCTCGATACCCACTTAACTTGCCGTGTGTATGATTTGAATTTGTTAGAAATCGACACCTCTGTTGCCCATCAAAGAT
TACAGGGTTACTTGCAAGCGATTGAAGAAGTGGGCGTTGTACTGGGGCAGGACCGCATTTGGAATATCCCCGAGAGCAAT
GCCGACTATGCGGCGATAGCGGCAAAAGAAGCGTTGAATTCGACTCCACGGCCGAATGTATTTTTATGCATGAGTGACTT
AATCGCCTTGAGTGTGCTCGGTGAGGCGCAGGCCATGGGGCTGAGTGTTCCTGAAGATGTGCGAGTAGTGGGCTTTGATG
GTATAGATGAAGGCACACGTTCAAATCCGCCGTTAACGACCGTCTATCAATACTCTGAGCAGAAGGGCCGCAAAGCGGCG
CAAATGTTTGTTAGTGACGCCGTGCATGCGGAAGTCTTGGGCTATGAATTACGTCTCGGTAAGAGCTGCTAA

Upstream 100 bases:

>100_bases
CGCATTAAGCCAAGATTTGCAGGTGCGCGACCCATTTAAGCCGTGTACTATTTGCGCTGAGCCGTATTTTTGTGGCCTTA
TTCGATTCCAGGACATAAGC

Downstream 100 bases:

>100_bases
TCGCTACATCTTTGTCCCGTCCTAAGATAGGTTGACAGTTTCTAGGCCAGCTCCAGTAGCTGGCCTTTTCTATTATGCAC
CTGATTGGGTGTTTCCATAT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTTTKHWTLKSIANELGVSNATVSNAFNRPDQLSEKRRNDILAACTKLGYFGPNKAAQSLRKGKFDTVALVLSDSVEYMV
SDPVASKFMKGVASVLEQEKLNLLLFSGSSDSVNAVADFVDGFICYGRPRNALLAEQLKQVKKKVVTVDFDIHRNASVSI
DNKLAAYEVAKLALQSPADNVAILGLRLLDTHLTCRVYDLNLLEIDTSVAHQRLQGYLQAIEEVGVVLGQDRIWNIPESN
ADYAAIAAKEALNSTPRPNVFLCMSDLIALSVLGEAQAMGLSVPEDVRVVGFDGIDEGTRSNPPLTTVYQYSEQKGRKAA
QMFVSDAVHAEVLGYELRLGKSC

Sequences:

>Translated_343_residues
MTTTKHWTLKSIANELGVSNATVSNAFNRPDQLSEKRRNDILAACTKLGYFGPNKAAQSLRKGKFDTVALVLSDSVEYMV
SDPVASKFMKGVASVLEQEKLNLLLFSGSSDSVNAVADFVDGFICYGRPRNALLAEQLKQVKKKVVTVDFDIHRNASVSI
DNKLAAYEVAKLALQSPADNVAILGLRLLDTHLTCRVYDLNLLEIDTSVAHQRLQGYLQAIEEVGVVLGQDRIWNIPESN
ADYAAIAAKEALNSTPRPNVFLCMSDLIALSVLGEAQAMGLSVPEDVRVVGFDGIDEGTRSNPPLTTVYQYSEQKGRKAA
QMFVSDAVHAEVLGYELRLGKSC
>Mature_342_residues
TTTKHWTLKSIANELGVSNATVSNAFNRPDQLSEKRRNDILAACTKLGYFGPNKAAQSLRKGKFDTVALVLSDSVEYMVS
DPVASKFMKGVASVLEQEKLNLLLFSGSSDSVNAVADFVDGFICYGRPRNALLAEQLKQVKKKVVTVDFDIHRNASVSID
NKLAAYEVAKLALQSPADNVAILGLRLLDTHLTCRVYDLNLLEIDTSVAHQRLQGYLQAIEEVGVVLGQDRIWNIPESNA
DYAAIAAKEALNSTPRPNVFLCMSDLIALSVLGEAQAMGLSVPEDVRVVGFDGIDEGTRSNPPLTTVYQYSEQKGRKAAQ
MFVSDAVHAEVLGYELRLGKSC

Specific function: This protein negatively controls the transcription initiation of genes such as deoCABD, udp, and cdd encoding catabolizing enzymes and nupC, nupG, and tsx encoding transporting and pore-forming proteins. Binds cytidine and adenosine as effectors [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=348, Percent_Identity=26.4367816091954, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1787948, Length=343, Percent_Identity=23.3236151603499, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994940, Length=351, Percent_Identity=23.9316239316239, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787580, Length=300, Percent_Identity=23, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790715, Length=358, Percent_Identity=23.463687150838, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37400; Mature: 37269

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTKHWTLKSIANELGVSNATVSNAFNRPDQLSEKRRNDILAACTKLGYFGPNKAAQSL
CCCCCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RKGKFDTVALVLSDSVEYMVSDPVASKFMKGVASVLEQEKLNLLLFSGSSDSVNAVADFV
HCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
DGFICYGRPRNALLAEQLKQVKKKVVTVDFDIHRNASVSIDNKLAAYEVAKLALQSPADN
CCEEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCC
VAILGLRLLDTHLTCRVYDLNLLEIDTSVAHQRLQGYLQAIEEVGVVLGQDRIWNIPESN
EEEEHHHHCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
ADYAAIAAKEALNSTPRPNVFLCMSDLIALSVLGEAQAMGLSVPEDVRVVGFDGIDEGTR
CCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
SNPPLTTVYQYSEQKGRKAAQMFVSDAVHAEVLGYELRLGKSC
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
>Mature Secondary Structure 
TTTKHWTLKSIANELGVSNATVSNAFNRPDQLSEKRRNDILAACTKLGYFGPNKAAQSL
CCCCCCHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
RKGKFDTVALVLSDSVEYMVSDPVASKFMKGVASVLEQEKLNLLLFSGSSDSVNAVADFV
HCCCCCEEEEEECCCHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
DGFICYGRPRNALLAEQLKQVKKKVVTVDFDIHRNASVSIDNKLAAYEVAKLALQSPADN
CCEEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCEEECCHHHHHHHHHHHHHCCCCC
VAILGLRLLDTHLTCRVYDLNLLEIDTSVAHQRLQGYLQAIEEVGVVLGQDRIWNIPESN
EEEEHHHHCCCCEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCC
ADYAAIAAKEALNSTPRPNVFLCMSDLIALSVLGEAQAMGLSVPEDVRVVGFDGIDEGTR
CCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
SNPPLTTVYQYSEQKGRKAAQMFVSDAVHAEVLGYELRLGKSC
CCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]