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The map label for this gene is rlmF [H]

Identifier: 126176412

GI number: 126176412

Start: 4953420

End: 4954547

Strand: Reverse

Name: rlmF [H]

Synonym: Sbal_4227

Alternate gene names: 126176412

Gene position: 4954547-4953420 (Counterclockwise)

Preceding gene: 126176413

Following gene: 126176407

Centisome position: 96.63

GC content: 50.0

Gene sequence:

>1128_bases
ATGTCGGCCCATTGCGTTACAAGAGCTGCTATGTCAAAACCTGCCGTGAAATCCGTTCCATCAGCGACTGCGAAAACCGC
AACCCGTGCAGTTAACATCCGCCAAAAAGTTAAAGCACCAAAGCAGGCTAAACCTGAAGCTAAAGGCAGAGCAAAGCCAA
GCAAAGACAAACCCAGAGCTGAAATCAAAAAAGCCCTGCATCCGCGTAATGCCCACTTGAATGGCTATGATTTTCCAGCG
CTATACGCTGCATTCACTCAGCTAAAAACCTTTGTGCGGCCCACGCCCTATGGCACGCTATCGATAGATTTTGCCGACCC
CTTGGCAGTCAAAACCCTCAATGCGGCGTTACTCAAACATCACTATGGAATTGGAGCTTGGGATATTCCTCAGGGAGCGT
TATGTCCTCCGATCCCCGGTCGCGTCGATTATGTGCATTACGTGGCAGATCTGTTAGCCGAAGGCGATAAGTCATGTGCC
ATGGATAAGGCGAGGGTGCTGGATATTGGCACGGGCGCGAACGGCATTTATCCCATATTGGGCTGCCAAGTGTACGGCTG
GCAATATGTTGCCTCGGATATCAATGCCCATTCACTCACCAATGTGCAGAGTATTATCGAGCAAAACCCAGTGCTGCAAG
GGCGGATAAGTTTACGATTACAGCCAGATGACAAGGCAGTATTCAAGGGAGTTATTCAAGCCGAAGAACGTTTTGAGCTC
ACCCTGTGTAATCCTCCGTTTCATGCCTCTATGGCCGAGGCGAGCGAAGGTACAAAGCGTAAGGTGAATAATCTACAGCT
TAATCGGGGGAGCTCAGTAAAGGCTGCACCTAAGCTGAATTTCGGCGGCCAAGCGGCAGAGCTTTGGTGCCAAGGTGGAG
AGCGACAGTTCTTGGCGACTATGATCCGCGAGAGTCAGATGTTTGCAGACCAATGCCTGTGGTTCACTAGCCTAGTGTCG
AAGCAAGAAAACCTGAAACCTTGTTATCAAGCCTTAGCACAGTTGAACGTCGATACTGTGAAGACGATTGAAATGCAGCA
AGGCAATAAAATTACGCGGGTCTTAGCGTGGAGCTTTCAGTCGGCCGCTAAGCGCAAAATCTGGCGCGCTGAACACTTAG
CCAATTAA

Upstream 100 bases:

>100_bases
AATCTGCTCACTCAAGAAGCGGGTAAAGCCTTGATTGTAAACTTACTCTTTACCTCTTATTTGTACGATTAATTTATTGT
CTTATTTTATGGGCTGACTG

Downstream 100 bases:

>100_bases
GATTTTCAGTTTACTTAATAACAAAACAGGCGCCATTATTGGCGCCTGTTTTTTAAAGGTAAAAAGAACCACTGTTACTG
CGGCTTTTCTTCCTGATGAT

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSAHCVTRAAMSKPAVKSVPSATAKTATRAVNIRQKVKAPKQAKPEAKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPA
LYAAFTQLKTFVRPTPYGTLSIDFADPLAVKTLNAALLKHHYGIGAWDIPQGALCPPIPGRVDYVHYVADLLAEGDKSCA
MDKARVLDIGTGANGIYPILGCQVYGWQYVASDINAHSLTNVQSIIEQNPVLQGRISLRLQPDDKAVFKGVIQAEERFEL
TLCNPPFHASMAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGERQFLATMIRESQMFADQCLWFTSLVS
KQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKIWRAEHLAN

Sequences:

>Translated_375_residues
MSAHCVTRAAMSKPAVKSVPSATAKTATRAVNIRQKVKAPKQAKPEAKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPA
LYAAFTQLKTFVRPTPYGTLSIDFADPLAVKTLNAALLKHHYGIGAWDIPQGALCPPIPGRVDYVHYVADLLAEGDKSCA
MDKARVLDIGTGANGIYPILGCQVYGWQYVASDINAHSLTNVQSIIEQNPVLQGRISLRLQPDDKAVFKGVIQAEERFEL
TLCNPPFHASMAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGERQFLATMIRESQMFADQCLWFTSLVS
KQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKIWRAEHLAN
>Mature_374_residues
SAHCVTRAAMSKPAVKSVPSATAKTATRAVNIRQKVKAPKQAKPEAKGRAKPSKDKPRAEIKKALHPRNAHLNGYDFPAL
YAAFTQLKTFVRPTPYGTLSIDFADPLAVKTLNAALLKHHYGIGAWDIPQGALCPPIPGRVDYVHYVADLLAEGDKSCAM
DKARVLDIGTGANGIYPILGCQVYGWQYVASDINAHSLTNVQSIIEQNPVLQGRISLRLQPDDKAVFKGVIQAEERFELT
LCNPPFHASMAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGERQFLATMIRESQMFADQCLWFTSLVSK
QENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQSAAKRKIWRAEHLAN

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=300, Percent_Identity=30.3333333333333, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI87081786, Length=304, Percent_Identity=48.6842105263158, Blast_Score=310, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI32564931, Length=310, Percent_Identity=26.4516129032258, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564933, Length=309, Percent_Identity=24.5954692556634, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281363415, Length=300, Percent_Identity=26.3333333333333, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922302, Length=300, Percent_Identity=26.3333333333333, Blast_Score=119, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 41248; Mature: 41117

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHCVTRAAMSKPAVKSVPSATAKTATRAVNIRQKVKAPKQAKPEAKGRAKPSKDKPRA
CCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
EIKKALHPRNAHLNGYDFPALYAAFTQLKTFVRPTPYGTLSIDFADPLAVKTLNAALLKH
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
HYGIGAWDIPQGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIGTGANGIYPIL
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCHHH
GCQVYGWQYVASDINAHSLTNVQSIIEQNPVLQGRISLRLQPDDKAVFKGVIQAEERFEL
CEEEECHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEE
TLCNPPFHASMAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGERQFLAT
EECCCCCCCHHHHHCCCHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHH
MIRESQMFADQCLWFTSLVSKQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEECCCCCEEHHHHHHHH
SAAKRKIWRAEHLAN
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAHCVTRAAMSKPAVKSVPSATAKTATRAVNIRQKVKAPKQAKPEAKGRAKPSKDKPRA
CCHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHH
EIKKALHPRNAHLNGYDFPALYAAFTQLKTFVRPTPYGTLSIDFADPLAVKTLNAALLKH
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHH
HYGIGAWDIPQGALCPPIPGRVDYVHYVADLLAEGDKSCAMDKARVLDIGTGANGIYPIL
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEEEECCCCCCCCHHH
GCQVYGWQYVASDINAHSLTNVQSIIEQNPVLQGRISLRLQPDDKAVFKGVIQAEERFEL
CEEEECHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHCCCEEE
TLCNPPFHASMAEASEGTKRKVNNLQLNRGSSVKAAPKLNFGGQAAELWCQGGERQFLAT
EECCCCCCCHHHHHCCCHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHH
MIRESQMFADQCLWFTSLVSKQENLKPCYQALAQLNVDTVKTIEMQQGNKITRVLAWSFQ
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEEECCCCCEEHHHHHHHH
SAAKRKIWRAEHLAN
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA