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The map label for this gene is nudE [H]

Identifier: 126176373

GI number: 126176373

Start: 4902357

End: 4902908

Strand: Reverse

Name: nudE [H]

Synonym: Sbal_4188

Alternate gene names: 126176373

Gene position: 4902908-4902357 (Counterclockwise)

Preceding gene: 126176374

Following gene: 126176372

Centisome position: 95.62

GC content: 47.64

Gene sequence:

>552_bases
ATGTCGCAGCGGCAGAATAAGCCGGAAATATTGCATACCGAAGTTGTCGCGAAAAGCCGATTATTTCAAATTGAGCAAGT
GCATCTTAAGTTCTCAAATGGTGTCGAACGCCAATACGAGCGCATGAAAGGCGGCAGCCGTGGCGCTGTGATGATAGTGC
CTATTCATCAAGGGCAAATGCTACTGGCGCGCGAATATGCTGCCGGAACCGATAATTACGAGCTCGGTTTTCCTAAAGGT
CTTATCGACCCTGGTGAATCGCCAATCGAAGCCGCTAACCGTGAATTGCAGGAAGAAATTGGCTTTGGGGCGAATAAGCT
CACGCTATTGAAAGAACTTAGCCTAGCGCCGGGGTATTTCTCCAGTAAGATGCAAATTTTTCTTGCAGAAGATCTGTATG
AGAGTCGCCTTGAAGGTGATGAGCCTGAGCCAATTGATGTGGTGCCTTGGGCGCTCGCCGATTGGGAAGCCTTGCTCGAT
GATGTCGATTTTTCTGAATCCCGTAGTGTGAGCGCGTTGTTTTTGGCGCAAAAATATTTACAACTTAAATAA

Upstream 100 bases:

>100_bases
TTTAGGTGTATGCAATAAATTCAGGTTGTAGATTAAAGACAGGATAGTAAGATTGTCACTTACCTTATTTAATTTATCGC
TTTTTTATCCAGAGGCAGTT

Downstream 100 bases:

>100_bases
TCTTTTTCCTTTTAAGCGTTCAGAGTTTGCACGCTTGCAAAACATTCGCTCAGCAGGCGATTGGAGTTGTTATGAAGCCA
GAAGAGTTGATCGACGAAGT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 183; Mature: 182

Protein sequence:

>183_residues
MSQRQNKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMIVPIHQGQMLLAREYAAGTDNYELGFPKG
LIDPGESPIEAANRELQEEIGFGANKLTLLKELSLAPGYFSSKMQIFLAEDLYESRLEGDEPEPIDVVPWALADWEALLD
DVDFSESRSVSALFLAQKYLQLK

Sequences:

>Translated_183_residues
MSQRQNKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMIVPIHQGQMLLAREYAAGTDNYELGFPKG
LIDPGESPIEAANRELQEEIGFGANKLTLLKELSLAPGYFSSKMQIFLAEDLYESRLEGDEPEPIDVVPWALADWEALLD
DVDFSESRSVSALFLAQKYLQLK
>Mature_182_residues
SQRQNKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMIVPIHQGQMLLAREYAAGTDNYELGFPKGL
IDPGESPIEAANRELQEEIGFGANKLTLLKELSLAPGYFSSKMQIFLAEDLYESRLEGDEPEPIDVVPWALADWEALLDD
VDFSESRSVSALFLAQKYLQLK

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=181, Percent_Identity=62.9834254143646, Blast_Score=230, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 20630; Mature: 20499

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRQNKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMIVPIHQGQM
CCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCE
LLAREYAAGTDNYELGFPKGLIDPGESPIEAANRELQEEIGFGANKLTLLKELSLAPGYF
EEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
SSKMQIFLAEDLYESRLEGDEPEPIDVVPWALADWEALLDDVDFSESRSVSALFLAQKYL
CCCEEEEEHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QLK
HCC
>Mature Secondary Structure 
SQRQNKPEILHTEVVAKSRLFQIEQVHLKFSNGVERQYERMKGGSRGAVMIVPIHQGQM
CCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCEEEEEEECCCCE
LLAREYAAGTDNYELGFPKGLIDPGESPIEAANRELQEEIGFGANKLTLLKELSLAPGYF
EEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
SSKMQIFLAEDLYESRLEGDEPEPIDVVPWALADWEALLDDVDFSESRSVSALFLAQKYL
CCCEEEEEHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
QLK
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]