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The map label for this gene is gcvA [H]

Identifier: 126176289

GI number: 126176289

Start: 4813067

End: 4813939

Strand: Direct

Name: gcvA [H]

Synonym: Sbal_4104

Alternate gene names: 126176289

Gene position: 4813067-4813939 (Clockwise)

Preceding gene: 126176287

Following gene: 126176293

Centisome position: 93.87

GC content: 49.26

Gene sequence:

>873_bases
ATGAGAAAGCTCCCACCATTACGCGCTCTGCAAGTGTTCGAAGCCGCCGCCAGACAACAGCATTTTTCTAAGGCGGCCGA
TGAACTGTGCATCACCCAAAGTGCGGTTTCTCACCAAGTGCGGGTATTAGAAGAGTATTTTGGCGAGCAACTGTTCGACC
GTCAGGGGCGTAAACTGCAGTTAACCCCAAAAGGACACGGACTATTTCTAGAGTTAGAGCGGATATTCAACGAGTTAAGT
GATCTTAATTTGAAGGTTCGCGACACGCCCAATCAGCAGCTGTGCTTAGCAGTTTACAGTTCCTTCTCGGTAAAATGGTT
GATCCCCAGACTCACCGATTTTCGCCGCCAATATCCGAGCGTGAAAATCCGCCTCGAGATGATCACCCAAGATCCTGATC
TCAGCACTAGTACGGCGGATATCTTTATCGCCGGTAAACTGAATCAACGCGGATACTGGCAACAGTTACTGCATAAAGAG
CGGTTAATCCCAGTATGCAGCCCAAATTTCTATCTTAAATGGCGCGACATTAGCCTGGGCAATTTCCAGCAACAAGCCCT
ACTGGTTGTCGATGAAGGCCCACTCGGACTCGACTGGCACAAGTGGTCATCGGCGCACCAAATCCCACTTGATAACGAAC
AACAGCATGTGTTTAGTCATATTGTGATGGCGATTGAGGCGGCGATTGCTGGTCAAGGCTTAGCCCTAGCGCCGGACTTT
ATGGTCGCTGGCGATATCACCACGGGGCGTTTAGTCGCTTTGAATCTTCCCGATGTGCATACCGGATTTGAATTTACTTT
CTTTTGTAAGCAACGCCGTTTATCAGAACCTGCGATTGCCGCCTTCTCCTCTTGGCTAAAATCACAAGCCTAA

Upstream 100 bases:

>100_bases
CAATGGCGGCATGATAATCCTCGCAAAACTTAAGCTGAAATGATGCTTTCTCATGCTAGATGAATTAGATTCATACTAAA
GCCTTTAGATATTGAAAATA

Downstream 100 bases:

>100_bases
GGTGAACAGACAGACACAAAAAAGCCGACGCTGACGTCGGCTTAATTTATCGATATCGATTGATTACACTAACAAACCAA
TCTTCTCGTACACTTTCTTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLTDFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLGNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA

Sequences:

>Translated_290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLTDFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLGNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
>Mature_290_residues
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQLTPKGHGLFLELERIFNELS
DLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLTDFRRQYPSVKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKE
RLIPVCSPNFYLKWRDISLGNFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=34.5890410958904, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1786448, Length=288, Percent_Identity=29.5138888888889, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1788706, Length=302, Percent_Identity=31.1258278145695, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1790208, Length=295, Percent_Identity=27.4576271186441, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=256, Percent_Identity=25.390625, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=23.8255033557047, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=25.2595155709343, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=127, Percent_Identity=33.0708661417323, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789440, Length=262, Percent_Identity=22.9007633587786, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33115; Mature: 33115

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPKGHGLFLELERIFNELSDLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLTDFRRQYPS
ECCCCCCEEEEHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCCC
VKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKERLIPVCSPNFYLKWRDISLG
EEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEEEEECCCC
NFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
CCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCE
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
EEECCCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLPPLRALQVFEAAARQQHFSKAADELCITQSAVSHQVRVLEEYFGEQLFDRQGRKLQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LTPKGHGLFLELERIFNELSDLNLKVRDTPNQQLCLAVYSSFSVKWLIPRLTDFRRQYPS
ECCCCCCEEEEHHHHHHHHHHCEEEEECCCCHHHHHHHHHCCCEEEEHHHHHHHHHHCCC
VKIRLEMITQDPDLSTSTADIFIAGKLNQRGYWQQLLHKERLIPVCSPNFYLKWRDISLG
EEEEEEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEECCCEEEEEEECCCC
NFQQQALLVVDEGPLGLDWHKWSSAHQIPLDNEQQHVFSHIVMAIEAAIAGQGLALAPDF
CCCCEEEEEEECCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEECCCE
MVAGDITTGRLVALNLPDVHTGFEFTFFCKQRRLSEPAIAAFSSWLKSQA
EEECCCCCCEEEEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]