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The map label for this gene is gntR [H]

Identifier: 126176275

GI number: 126176275

Start: 4797865

End: 4798863

Strand: Reverse

Name: gntR [H]

Synonym: Sbal_4089

Alternate gene names: 126176275

Gene position: 4798863-4797865 (Counterclockwise)

Preceding gene: 126176276

Following gene: 126176273

Centisome position: 93.59

GC content: 47.55

Gene sequence:

>999_bases
ATGAATAAAAAGCGTAGACCCACTCTTCAAGACGTTGCCGATCAAGTTGGGATCACCAAAATGACGGTGAGCCGCTATTT
AAAAAATCCAACAAGGGTGGCGCAAAGCACTCAAGAAAAGATAGCTATCGCGCTAGAAACCTTAGGTTATATTCCTAATC
GCGCGCCGGAAATTTTGGCGCAAGCAACCAGCCGCGCCATTGGCGTGTTAGTACCTTCGTTAACCAACCAAGTGTTTGCC
GAAGTGCTGCGCGGTATCGAGAATGTGACCGGCAAACGCGGTTATCAAACCATGCTGGCTCACTACGGATATCAGGCAGA
ATTAGAAGAGGAGCGGGTGGCATCACTGCTTTCTTACAATATTGATGGCTTACTTTTATCCGAAAGTTACCACACGCCGC
GTACCGTGCGAATGATTGAAACGGCGGGCATTCCGGTCATTGAAATGATGGATAGTGTGTCGCCTTCAATTCAACAGTGT
GTGGGGTTCGATAACACAGCTGCCGCATACGAAATGGTTAAGGCCATGATTGCACAAGGCTATCGCCACATTGCTTACTT
AGGCGCGCGCATGGATATTCGCACTCAGCTTAAGATGCGAGGCTACGAGTTAGCGATGCAGGAAGCCAAGCTCACACCTT
TGACGATGACCACCAATGAGGCTTCATCGTTTTCCTTGGGGGCGTCGTTATTGCAACAAACCTTAGCGAAATATCCCTAT
GTGGATAGCTTTTTCTGCACTAACGATGATTTGGCTGCGGGGGTGATATTTGAGTGTCAAAGACAAGGCATTAAGATCCC
AGAACAAATAGGCGTTGCAGGGTTTCATGGCCATGATATTGGGCAATCCATGACGCCAAAATTGGCGAGCATTATTACCC
CGAGAAAGGAGATCGGTCGAATTGCTGCCACTGAGTTATTAGACCGATTAAATGGCAAAGAGATAGCAGAATCGGTTATC
GATCTGGGCTATCGTATTGATCTAGGGGAAACATTGTAG

Upstream 100 bases:

>100_bases
AAAATCTCGTCTGATGCTTATGTCTACTATTTGTTTATATTCGATATTTTAGCTATACATCTCACCCATATCCGCGGCTT
GTTAACTAACATTAGATACT

Downstream 100 bases:

>100_bases
ACTTCCCCTCGATCTTTCGCTTAACGGCTAATGCCTAAGGTTTTTGATCTCTGGCTAGCGTTAGAGAAACGCTGAGATGA
TGACTGCAACTATGCCGCCC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNKKRRPTLQDVADQVGITKMTVSRYLKNPTRVAQSTQEKIAIALETLGYIPNRAPEILAQATSRAIGVLVPSLTNQVFA
EVLRGIENVTGKRGYQTMLAHYGYQAELEEERVASLLSYNIDGLLLSESYHTPRTVRMIETAGIPVIEMMDSVSPSIQQC
VGFDNTAAAYEMVKAMIAQGYRHIAYLGARMDIRTQLKMRGYELAMQEAKLTPLTMTTNEASSFSLGASLLQQTLAKYPY
VDSFFCTNDDLAAGVIFECQRQGIKIPEQIGVAGFHGHDIGQSMTPKLASIITPRKEIGRIAATELLDRLNGKEIAESVI
DLGYRIDLGETL

Sequences:

>Translated_332_residues
MNKKRRPTLQDVADQVGITKMTVSRYLKNPTRVAQSTQEKIAIALETLGYIPNRAPEILAQATSRAIGVLVPSLTNQVFA
EVLRGIENVTGKRGYQTMLAHYGYQAELEEERVASLLSYNIDGLLLSESYHTPRTVRMIETAGIPVIEMMDSVSPSIQQC
VGFDNTAAAYEMVKAMIAQGYRHIAYLGARMDIRTQLKMRGYELAMQEAKLTPLTMTTNEASSFSLGASLLQQTLAKYPY
VDSFFCTNDDLAAGVIFECQRQGIKIPEQIGVAGFHGHDIGQSMTPKLASIITPRKEIGRIAATELLDRLNGKEIAESVI
DLGYRIDLGETL
>Mature_332_residues
MNKKRRPTLQDVADQVGITKMTVSRYLKNPTRVAQSTQEKIAIALETLGYIPNRAPEILAQATSRAIGVLVPSLTNQVFA
EVLRGIENVTGKRGYQTMLAHYGYQAELEEERVASLLSYNIDGLLLSESYHTPRTVRMIETAGIPVIEMMDSVSPSIQQC
VGFDNTAAAYEMVKAMIAQGYRHIAYLGARMDIRTQLKMRGYELAMQEAKLTPLTMTTNEASSFSLGASLLQQTLAKYPY
VDSFFCTNDDLAAGVIFECQRQGIKIPEQIGVAGFHGHDIGQSMTPKLASIITPRKEIGRIAATELLDRLNGKEIAESVI
DLGYRIDLGETL

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=330, Percent_Identity=60, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=47.7341389728097, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1790369, Length=313, Percent_Identity=29.3929712460064, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1790194, Length=307, Percent_Identity=26.3843648208469, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788474, Length=303, Percent_Identity=25.0825082508251, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789068, Length=307, Percent_Identity=25.7328990228013, Blast_Score=104, Evalue=9e-24,
Organism=Escherichia coli, GI1787948, Length=317, Percent_Identity=24.2902208201893, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789202, Length=290, Percent_Identity=23.7931034482759, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787906, Length=340, Percent_Identity=25.5882352941176, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=24.9211356466877, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=23.5668789808917, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=23.3644859813084, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786268, Length=286, Percent_Identity=23.4265734265734, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36642; Mature: 36642

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKRRPTLQDVADQVGITKMTVSRYLKNPTRVAQSTQEKIAIALETLGYIPNRAPEILA
CCCCCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QATSRAIGVLVPSLTNQVFAEVLRGIENVTGKRGYQTMLAHYGYQAELEEERVASLLSYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
IDGLLLSESYHTPRTVRMIETAGIPVIEMMDSVSPSIQQCVGFDNTAAAYEMVKAMIAQG
CCCEEEECCCCCCHHEEEHHHCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHH
YRHIAYLGARMDIRTQLKMRGYELAMQEAKLTPLTMTTNEASSFSLGASLLQQTLAKYPY
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
VDSFFCTNDDLAAGVIFECQRQGIKIPEQIGVAGFHGHDIGQSMTPKLASIITPRKEIGR
CCCEEECCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHH
IAATELLDRLNGKEIAESVIDLGYRIDLGETL
HHHHHHHHHCCHHHHHHHHHHCCCEECCCCCC
>Mature Secondary Structure
MNKKRRPTLQDVADQVGITKMTVSRYLKNPTRVAQSTQEKIAIALETLGYIPNRAPEILA
CCCCCCCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QATSRAIGVLVPSLTNQVFAEVLRGIENVTGKRGYQTMLAHYGYQAELEEERVASLLSYN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCC
IDGLLLSESYHTPRTVRMIETAGIPVIEMMDSVSPSIQQCVGFDNTAAAYEMVKAMIAQG
CCCEEEECCCCCCHHEEEHHHCCCHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHH
YRHIAYLGARMDIRTQLKMRGYELAMQEAKLTPLTMTTNEASSFSLGASLLQQTLAKYPY
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHCCC
VDSFFCTNDDLAAGVIFECQRQGIKIPEQIGVAGFHGHDIGQSMTPKLASIITPRKEIGR
CCCEEECCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHCCHHHHHH
IAATELLDRLNGKEIAESVIDLGYRIDLGETL
HHHHHHHHHCCHHHHHHHHHHCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]