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The map label for this gene is pucG [H]

Identifier: 126176213

GI number: 126176213

Start: 4725859

End: 4726992

Strand: Reverse

Name: pucG [H]

Synonym: Sbal_4027

Alternate gene names: 126176213

Gene position: 4726992-4725859 (Counterclockwise)

Preceding gene: 126176214

Following gene: 126176212

Centisome position: 92.19

GC content: 51.94

Gene sequence:

>1134_bases
ATGTTACCAGCACCGCGTTTTACCGCTTTTAATCCTCCGCGCCGTATTTTGATGGGCCCTGGCCCGTCGGATGTTTATCC
CGAAGTGCTTGCGGCGCAGTCCAGACCGACAATTGGCCACTTAGATCCGCTATTTGTCGGCATGATGGATGAGCTTAAGA
GCCTGATCCAATATGCCTTCCAAACTAAAAACGAAATGACCATGGCAGTATCCGCGCCGGGCAGTGCGGGCATGGAAACC
TGTTTCGTAAACTTAGTCGAGCCGGGTGAGAAAGTTATTGTCTGTCGTAACGGTGTGTTTGGTGAGCGTATGCGCCAAAA
CGTCGAGCGCGTTGGCGCCATTGCAGTGCTGGTGGACAATGAGTGGGGCGCCCCTGTCGACCCTGCTGCGGTTGAAGCTG
CGCTGAAAGCCAATCCCGATGCCAAATTTTTAGCCTTTGTGCACGCAGAAACCTCTACTGGCGCGCTGTCCGATGCCAAA
ACCTTGTGTGCATTAGCCAAACAATACGGCTGCTTATCAATCGTCGATGCTGTGACTTCCCTTGGCGGTGTCGAGTTAAG
AGTCGATGAATGGGGCATAGATGCCATTTATTCCGGCAGTCAAAAGTGTCTTTCCTGTGTACCAGGTTTATCACCTGTGT
CTTTCTCGATCGCAGCGGTAGAGAAACTCAAGAATCGTAAAACCCCAGTGCAAAGTTGGTTCCTCGATCAAAGTCTAGTG
ATGGCCTATTGGACCAGCGCGGGCGGCAAACGCAGTTATCATCACACTGCGCCAGTGAACGCGCTTTATGCACTGCATGA
ATCCCTGCGTATTTTGGCGGCTGAAGGCTTAGAGAATGCGTGGCAACGCCATCACGAAATGCATTTAGTGCTGCGTGCTG
GGCTGGAAAAGTTAGGGCTTAATTTTGTGGTGGCCGAAGATTATCGCCTACCGCAGCTGAATACGGTTTATATCCCAGCA
GGTGTCGATGATGCCGCAGTGCGGACTCGTTTACTGAAGGATTACAACCTCGAAATCGGCGCAGGCCTTGGCGCCCTTGC
GGGTAAAGCATGGCGTATCGGTCTAATGGGCTTTGGTGCCCGCCGTGAGAATGTCTCTTTATGCCTCAAGGCATTAGAAG
AAGTGTTGAACTAA

Upstream 100 bases:

>100_bases
TTACTGACTTAACCCGTTAACGATTAACTGGCAGTTTCTGAAAACAGAACGGCTAACGAATAACAACGAGACCTCGACCT
GCACGCATTAAGGAGACATG

Downstream 100 bases:

>100_bases
TATGCCAAGATTACGCCTTGCCCTTTGCGGCCTAATGATCGCTCTGCTCAGTGCCTGTTCGGTGAACAATACCAGCAATG
CGTCCTGCGATTTTGTACGC

Product: Serine--pyruvate transaminase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MLPAPRFTAFNPPRRILMGPGPSDVYPEVLAAQSRPTIGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGAPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
TLCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSIAAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRILAAEGLENAWQRHHEMHLVLRAGLEKLGLNFVVAEDYRLPQLNTVYIPA
GVDDAAVRTRLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVSLCLKALEEVLN

Sequences:

>Translated_377_residues
MLPAPRFTAFNPPRRILMGPGPSDVYPEVLAAQSRPTIGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGAPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
TLCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSIAAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRILAAEGLENAWQRHHEMHLVLRAGLEKLGLNFVVAEDYRLPQLNTVYIPA
GVDDAAVRTRLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVSLCLKALEEVLN
>Mature_377_residues
MLPAPRFTAFNPPRRILMGPGPSDVYPEVLAAQSRPTIGHLDPLFVGMMDELKSLIQYAFQTKNEMTMAVSAPGSAGMET
CFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDNEWGAPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAK
TLCALAKQYGCLSIVDAVTSLGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSIAAVEKLKNRKTPVQSWFLDQSLV
MAYWTSAGGKRSYHHTAPVNALYALHESLRILAAEGLENAWQRHHEMHLVLRAGLEKLGLNFVVAEDYRLPQLNTVYIPA
GVDDAAVRTRLLKDYNLEIGAGLGALAGKAWRIGLMGFGARRENVSLCLKALEEVLN

Specific function: Could encode ureidoglycolase or L-alanine:glyoxylate aminotransferase, or perhaps both activities [H]

COG id: COG0075

COG function: function code E; Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557289, Length=376, Percent_Identity=39.3617021276596, Blast_Score=266, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17536281, Length=368, Percent_Identity=39.945652173913, Blast_Score=254, Evalue=4e-68,
Organism=Saccharomyces cerevisiae, GI6321079, Length=372, Percent_Identity=27.4193548387097, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI17530823, Length=368, Percent_Identity=39.6739130434783, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 2.6.1.-

Molecular weight: Translated: 40834; Mature: 40834

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPAPRFTAFNPPRRILMGPGPSDVYPEVLAAQSRPTIGHLDPLFVGMMDELKSLIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTKNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEEC
EWGAPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAKTLCALAKQYGCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSIAAVEKLKNRKTPVQSWFLDQSLV
CCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MAYWTSAGGKRSYHHTAPVNALYALHESLRILAAEGLENAWQRHHEMHLVLRAGLEKLGL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFVVAEDYRLPQLNTVYIPAGVDDAAVRTRLLKDYNLEIGAGLGALAGKAWRIGLMGFGA
EEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEECCCHHHHCCCCEEEEEEECCC
RRENVSLCLKALEEVLN
CCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MLPAPRFTAFNPPRRILMGPGPSDVYPEVLAAQSRPTIGHLDPLFVGMMDELKSLIQYAF
CCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
QTKNEMTMAVSAPGSAGMETCFVNLVEPGEKVIVCRNGVFGERMRQNVERVGAIAVLVDN
HCCCCEEEEEECCCCCCHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHHHCEEEEEEEC
EWGAPVDPAAVEAALKANPDAKFLAFVHAETSTGALSDAKTLCALAKQYGCLSIVDAVTS
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
LGGVELRVDEWGIDAIYSGSQKCLSCVPGLSPVSFSIAAVEKLKNRKTPVQSWFLDQSLV
CCCEEEEEECCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
MAYWTSAGGKRSYHHTAPVNALYALHESLRILAAEGLENAWQRHHEMHLVLRAGLEKLGL
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NFVVAEDYRLPQLNTVYIPAGVDDAAVRTRLLKDYNLEIGAGLGALAGKAWRIGLMGFGA
EEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEECCCHHHHCCCCEEEEEEECCC
RRENVSLCLKALEEVLN
CCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11344136 [H]