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The map label for this gene is flbD [H]

Identifier: 126176169

GI number: 126176169

Start: 4669480

End: 4670781

Strand: Reverse

Name: flbD [H]

Synonym: Sbal_3981

Alternate gene names: 126176169

Gene position: 4670781-4669480 (Counterclockwise)

Preceding gene: 126176179

Following gene: 126176168

Centisome position: 91.09

GC content: 45.16

Gene sequence:

>1302_bases
GTGGGTGGCCTAAGTATACGGATTGAAGGATTAAACATATCTGCGGAACTTGCATGCACAATCGCACAGGGAGATTTTTA
TCTGTGGAATGATGCAATGGAGTCGTCTTGGCTCACGATCGTTAACTTATCAGATTGTCGTCCTTGTGATGTAAAATCTT
ATCTTGCCCGTTTTCCTTGCCGTAATATTGTCGCGTTATTAGCGCCAGAACAAGGTGAGTTAGCCGCGGCGGCAATGCGT
GCTGGTGTACAAGATTATCTATTAATTCCCGTAGAAACCGAGCAACTGCTAGCCTCAATACAACGTTTACGCCGTTTAGA
GTTACCTGATTCTAGTTTGGTTGTATCGGCTGCTGTTAGCCGGCAACTATTGATGTTGGCCCATAGGGCAGCAACCACTG
AGGCCAGTGTTTTATTATTGGGTGAAAGTGGTACTGGCAAGGAGCCGCTTGCTCGTTATATTCATCGTCATTCTTCTCGT
TCTCATAAGCCTTTCGTTGCGATTAACTGCGCCGCCATCCCTGAAAGTATTCTTGAATCTGTCTTATTTGGTCATGTTAA
AGGCGCCTTTACTGGTGCTATTTGCGATAAAGCAGGCAAATTTGAGCAGGCAAATGGCGGCACTTTATTACTCGATGAAA
TTGGTGAAATGCCCTTGCCGTTGCAGGCAAAATTACTCCGAGTGTTACAGGAGCGTGAAGTCGAAAGACTCGGCGGGCAG
CATGCTATTCCACTGGATATCAGAATCATTGCCTCTACCAATCGCGACTTAAGACAGGCCGTTGAGTTTGGGCATTTTAG
GGAGGATCTGTTCTACCGTTTAGATGTTTTGCCTTTAAAAATCGCGCCTTTAAGAGAACGTAAGGCTGACATCCTCCCTT
TAGCTGAGCATTTCTTAGGTTTATATGGTCAGTCCGATAACGCAAGTCGTTGCTATTTCAGTGAGCACGCAAGGCAAGTG
CTTGTTACCTATGATTGGCCGGGAAATGTTAGAGAGCTAGAAAATTGTATTCAGCGGGCATTAGTGATGCGCAGAGGTCA
GGCTATTCAAGTTGCTGAACTCGGATTAAACATTCAAGAAGAAACGCTCGAGTTAGAGCCTTTGGGCGCGACGGTAGGAC
TTAAAGCTTCAAAACAGCAGGCAGAATTTCAATATATTATTGATGTACTTAAACGTTTTAATGGACAACGCACTTTAAGT
GCGCAGGCGCTAGGGATGACGACTAGAGCCTTAAGATATCGCCTCGTGCAAATGCGAGAAGCAGGGATTGATATTGAAAC
GTTATTAGATCAAGCAGCGTGA

Upstream 100 bases:

>100_bases
TAATAAGCTGTAATAGTTTTTATATATATTCAAGCACGGACACTTATCGGTCTAAATAAAGGTGTTATTAAATGTACTGT
AAACGAGAGGGAGTTGGCGA

Downstream 100 bases:

>100_bases
GCAATCAAAAAAGTAATAGGAATGGAATATGTCTAATGGAGCGATTGGTAGCTCAAATATGTTGATGGAAACCTTAAATA
TTCATCGTGAAGTGGCTAAA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MGGLSIRIEGLNISAELACTIAQGDFYLWNDAMESSWLTIVNLSDCRPCDVKSYLARFPCRNIVALLAPEQGELAAAAMR
AGVQDYLLIPVETEQLLASIQRLRRLELPDSSLVVSAAVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSR
SHKPFVAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLPLQAKLLRVLQEREVERLGGQ
HAIPLDIRIIASTNRDLRQAVEFGHFREDLFYRLDVLPLKIAPLRERKADILPLAEHFLGLYGQSDNASRCYFSEHARQV
LVTYDWPGNVRELENCIQRALVMRRGQAIQVAELGLNIQEETLELEPLGATVGLKASKQQAEFQYIIDVLKRFNGQRTLS
AQALGMTTRALRYRLVQMREAGIDIETLLDQAA

Sequences:

>Translated_433_residues
MGGLSIRIEGLNISAELACTIAQGDFYLWNDAMESSWLTIVNLSDCRPCDVKSYLARFPCRNIVALLAPEQGELAAAAMR
AGVQDYLLIPVETEQLLASIQRLRRLELPDSSLVVSAAVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSR
SHKPFVAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLPLQAKLLRVLQEREVERLGGQ
HAIPLDIRIIASTNRDLRQAVEFGHFREDLFYRLDVLPLKIAPLRERKADILPLAEHFLGLYGQSDNASRCYFSEHARQV
LVTYDWPGNVRELENCIQRALVMRRGQAIQVAELGLNIQEETLELEPLGATVGLKASKQQAEFQYIIDVLKRFNGQRTLS
AQALGMTTRALRYRLVQMREAGIDIETLLDQAA
>Mature_432_residues
GGLSIRIEGLNISAELACTIAQGDFYLWNDAMESSWLTIVNLSDCRPCDVKSYLARFPCRNIVALLAPEQGELAAAAMRA
GVQDYLLIPVETEQLLASIQRLRRLELPDSSLVVSAAVSRQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSRS
HKPFVAINCAAIPESILESVLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLPLQAKLLRVLQEREVERLGGQH
AIPLDIRIIASTNRDLRQAVEFGHFREDLFYRLDVLPLKIAPLRERKADILPLAEHFLGLYGQSDNASRCYFSEHARQVL
VTYDWPGNVRELENCIQRALVMRRGQAIQVAELGLNIQEETLELEPLGATVGLKASKQQAEFQYIIDVLKRFNGQRTLSA
QALGMTTRALRYRLVQMREAGIDIETLLDQAA

Specific function: Activation of sigma-54-dependent flagellar gene promoters and strong negative autoregulatory effects on its own promoter. The synthesis and function of flbD in C.crescentus is controlled by an internal cell-cycle clock [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=396, Percent_Identity=43.4343434343434, Blast_Score=291, Evalue=4e-80,
Organism=Escherichia coli, GI87082117, Length=313, Percent_Identity=48.8817891373802, Blast_Score=268, Evalue=6e-73,
Organism=Escherichia coli, GI1790437, Length=426, Percent_Identity=38.0281690140845, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1788905, Length=352, Percent_Identity=41.7613636363636, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790299, Length=325, Percent_Identity=40, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=43.4920634920635, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=43.5185185185185, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082152, Length=215, Percent_Identity=53.953488372093, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=45.1923076923077, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1787583, Length=236, Percent_Identity=42.7966101694915, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI87081872, Length=219, Percent_Identity=44.7488584474886, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789828, Length=229, Percent_Identity=36.6812227074236, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI87081858, Length=339, Percent_Identity=29.4985250737463, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48033; Mature: 47902

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLSIRIEGLNISAELACTIAQGDFYLWNDAMESSWLTIVNLSDCRPCDVKSYLARFPC
CCCCEEEEEECEEEEEEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHCCH
RNIVALLAPEQGELAAAAMRAGVQDYLLIPVETEQLLASIQRLRRLELPDSSLVVSAAVS
HHHHEEECCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSRSHKPFVAINCAAIPESILES
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHH
VLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLPLQAKLLRVLQEREVERLGGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
HAIPLDIRIIASTNRDLRQAVEFGHFREDLFYRLDVLPLKIAPLRERKADILPLAEHFLG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHCCCCHHHHHHHHHH
LYGQSDNASRCYFSEHARQVLVTYDWPGNVRELENCIQRALVMRRGQAIQVAELGLNIQE
HCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHH
ETLELEPLGATVGLKASKQQAEFQYIIDVLKRFNGQRTLSAQALGMTTRALRYRLVQMRE
HHEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AGIDIETLLDQAA
CCCCHHHHHHHCC
>Mature Secondary Structure 
GGLSIRIEGLNISAELACTIAQGDFYLWNDAMESSWLTIVNLSDCRPCDVKSYLARFPC
CCCEEEEEECEEEEEEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHCCH
RNIVALLAPEQGELAAAAMRAGVQDYLLIPVETEQLLASIQRLRRLELPDSSLVVSAAVS
HHHHEEECCCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RQLLMLAHRAATTEASVLLLGESGTGKEPLARYIHRHSSRSHKPFVAINCAAIPESILES
HHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEHHHCCHHHHHH
VLFGHVKGAFTGAICDKAGKFEQANGGTLLLDEIGEMPLPLQAKLLRVLQEREVERLGGQ
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
HAIPLDIRIIASTNRDLRQAVEFGHFREDLFYRLDVLPLKIAPLRERKADILPLAEHFLG
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHCCCCHHHHHHHHHH
LYGQSDNASRCYFSEHARQVLVTYDWPGNVRELENCIQRALVMRRGQAIQVAELGLNIQE
HCCCCCCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCCHH
ETLELEPLGATVGLKASKQQAEFQYIIDVLKRFNGQRTLSAQALGMTTRALRYRLVQMRE
HHEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHH
AGIDIETLLDQAA
CCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2315326; 11259647 [H]