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The map label for this gene is outD [H]

Identifier: 126176026

GI number: 126176026

Start: 4507309

End: 4508988

Strand: Reverse

Name: outD [H]

Synonym: Sbal_3835

Alternate gene names: 126176026

Gene position: 4508988-4507309 (Counterclockwise)

Preceding gene: 126176027

Following gene: 126176025

Centisome position: 87.94

GC content: 47.98

Gene sequence:

>1680_bases
ATGAAAGCGATTAAATACTTTACACCTCTGCTCTCACTTTGCCTTGTGGCGTGTCAGACCACAAACAGGCCACAGCCGGA
GGCGAGTAAGGCGGAATTAGCGGCCTCAATGGCGACGGCTACAGCATCAACGACGCCACCGCCTGCGACTATGCCGGATG
CGGTTCAGCGAGAATTGAATTCGGGCGCTTTACTGTCAGGTTTAACCCCTGCGCTGGAAACTGAGCGTCGTTTTGATGTC
TCTGCCCATGATGTGGATGCCAAAGTATTTTTTCCAAGCTTAGTCCAAGGTACGCCGCTGAGTGTTGCCGTGCATCCTGA
TGTGCAAGGTACGATTTCGCTATCCCTTAAAGGTGTAACCTTAAGCGAAGCGATTCAAGTGGTTGAAGATATCTACGGTT
ACGAAGTGAGCCGTGAAGGGCGTATTCTAAGAGTCTTCCCCGCTGGAATGCGTACTGAGACTTTCCCGCTGAATTATCTT
TATATGGAGCGCGACGGCTTATCAGTGACCTCAGTGAGTTCTGGACGCATTTCCGATAGCAATAATTCCAATAGTAATTC
GAGCAATAACAACTCAAATAACAGTGGTTCGAGTAATAATAATTCCAACAGCAGTAATGGTAGTAACTCCAGCAGCAATG
GCAGCAGCGATAGCAGTAACGGTACCTTTATCCGCTCGCGCACTAAGACTGATTTTTGGGGCGAGCTAAAAGAAACGCTG
ACGGCGATTATTGGTGACACAGGTAATGGGCGGCAAGTGGTGGTCACACCGCAGGCGGGTTTAGTGACCATTCGTGCTTT
CCCAAGTGAATTACGCCAAGTTCGCACTTTCTTAAATTCTGCCGAGAGTCATCTGCAGCGCCAAGTGATCTTGGAGGCTA
AGATCATCGAAGTCACTTTATCTGATGGCTATCAGCAGGGCATCCAATGGGACAATGTTCTTGGTCATGTTGGCAATACG
AATGTGAATTTTGGGACTTCGAAAGGCCCTGGTTTAAGCGATAAAATCACCTCGGCGATTGGTGGCGTGACCTCGCTAAG
CATTAAAGGCTCCGACTTTACGACCATGATCAATCTGTTAGATACCCAAGGCGATGTGGATGTATTGTCTAGCCCAAGGG
TGACTGCATCGAATAACCAAAAAGCGGTGATTAAAGTCGGTACTGATGAGTACTTTGTCACTGACGTTTCATCGACGACA
GTTGGTGGCACGCCGCCTGTGACCACTCCGCAAGTCGAGCTTACGCCGTTTTTCTCAGGCATTGCCTTAGATGTCACGCC
GCAAATTGATAGTGACGGCAATGTGTTATTGCACGTGCATCCATCCGTTATCGATGTGAAAGAGCAGACTAAAGACATCA
AAGTGAGTGACGCTTCGTTGGAGCTACCGTTAGCACAGAGTGAAATTCGCGAGTCTGATACCGTTATCCGCGCCGCTTCC
GGTGACGTGGTCATCATCGGTGGCTTGATGAAGAGTGAAAGCATTGAAGTGGTATCGCAAGTGCCTTTGTTGGGTGATAT
TCCCTACCTCGGTGAATTGTTTAAAAACCGTAGCAAGCAAAAGAAAAAAACCGAGCTGATTATTCTGTTAAAACCTACGG
TAGTGGGCGTCGATACGTGGAAAACTGAGCTACAACGTTCTAAGACATTATTAGATCGTTGGTATCCGGAGACAAAGTAA

Upstream 100 bases:

>100_bases
AGAGTATCGTCAAGATCACCCCCACTGATGTGTCGCTGTCCGATGGACGGAAATTGACATTGTTTCAAGCAATAACAGAG
AGATAGGGAATACACCTCAG

Downstream 100 bases:

>100_bases
GCTTCTATGTATCAGAAGCATTTTGGCTTAAACCAAGCCCCATTTTCATTGACACCCAATACCGGATTTTTTTTCGGCCT
ATCGCCACACGTAGAAGCCT

Product: pilus (MSHA type) biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALETERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNNSNSSNGSNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWDNVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VGGTPPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSESIEVVSQVPLLGDIPYLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK

Sequences:

>Translated_559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALETERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNNSNSSNGSNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWDNVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VGGTPPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSESIEVVSQVPLLGDIPYLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK
>Mature_559_residues
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELNSGALLSGLTPALETERRFDV
SAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVTLSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYL
YMERDGLSVTSVSSGRISDSNNSNSNSSNNNSNNSGSSNNNSNSSNGSNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTLSDGYQQGIQWDNVLGHVGNT
NVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTT
VGGTPPVTTPQVELTPFFSGIALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
GDVVIIGGLMKSESIEVVSQVPLLGDIPYLGELFKNRSKQKKKTELIILLKPTVVGVDTWKTELQRSKTLLDRWYPETK

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=277, Percent_Identity=27.7978339350181, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789793, Length=272, Percent_Identity=27.9411764705882, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 59942; Mature: 59942

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
SGALLSGLTPALETERRFDVSAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVT
CCCEECCCCHHHCCCCCCCCCCCCCCCEEECHHHHCCCCEEEEECCCCCEEEEEEEECCC
LSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYLYMERDGLSVTSVSSGRISDS
HHHHHHHHHHHHCCEECCCCCEEEEECCCCCCCEECEEEEEEECCCCEEEEECCCCCCCC
NNSNSNSSNNNSNNSGSSNNNSNSSNGSNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTL
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
SDGYQQGIQWDNVLGHVGNTNVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLL
CCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHH
DTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVGGTPPVTTPQVELTPFFSG
CCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
IALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
EEEEEECCCCCCCCEEEEECCCEEECHHHCCCEEEECCEEECCCCHHHHCCCCCEEEECC
GDVVIIGGLMKSESIEVVSQVPLLGDIPYLGELFKNRSKQKKKTELIILLKPTVVGVDTW
CCEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHH
KTELQRSKTLLDRWYPETK
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKAIKYFTPLLSLCLVACQTTNRPQPEASKAELAASMATATASTTPPPATMPDAVQRELN
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHC
SGALLSGLTPALETERRFDVSAHDVDAKVFFPSLVQGTPLSVAVHPDVQGTISLSLKGVT
CCCEECCCCHHHCCCCCCCCCCCCCCCEEECHHHHCCCCEEEEECCCCCEEEEEEEECCC
LSEAIQVVEDIYGYEVSREGRILRVFPAGMRTETFPLNYLYMERDGLSVTSVSSGRISDS
HHHHHHHHHHHHCCEECCCCCEEEEECCCCCCCEECEEEEEEECCCCEEEEECCCCCCCC
NNSNSNSSNNNSNNSGSSNNNSNSSNGSNSSSNGSSDSSNGTFIRSRTKTDFWGELKETL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
TAIIGDTGNGRQVVVTPQAGLVTIRAFPSELRQVRTFLNSAESHLQRQVILEAKIIEVTL
HHHHCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEE
SDGYQQGIQWDNVLGHVGNTNVNFGTSKGPGLSDKITSAIGGVTSLSIKGSDFTTMINLL
CCCHHHCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCEEHHHHH
DTQGDVDVLSSPRVTASNNQKAVIKVGTDEYFVTDVSSTTVGGTPPVTTPQVELTPFFSG
CCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCC
IALDVTPQIDSDGNVLLHVHPSVIDVKEQTKDIKVSDASLELPLAQSEIRESDTVIRAAS
EEEEEECCCCCCCCEEEEECCCEEECHHHCCCEEEECCEEECCCCHHHHCCCCCEEEECC
GDVVIIGGLMKSESIEVVSQVPLLGDIPYLGELFKNRSKQKKKTELIILLKPTVVGVDTW
CCEEEEECCCCCCCHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECHHH
KTELQRSKTLLDRWYPETK
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]