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The map label for this gene is ycgF [H]

Identifier: 126175989

GI number: 126175989

Start: 4457815

End: 4458588

Strand: Reverse

Name: ycgF [H]

Synonym: Sbal_3798

Alternate gene names: 126175989

Gene position: 4458588-4457815 (Counterclockwise)

Preceding gene: 126175990

Following gene: 126175988

Centisome position: 86.96

GC content: 44.7

Gene sequence:

>774_bases
ATGGATTTCCCCGCTCGAATAAAGCAGCAAACATGCATGGATTGCTTAAGTGGTAAGTCATTAGGATTTGAAATTCGCAT
GGCATTTCAACCTATCATTCATTGGCCTAGCCGCGAGATAAGCGGTTATGAAGCCCTTGTTAGAGGTCCAGAAGGTGAAG
GGGCTGCGTGGGTATTTGCGCGAATTAACGATAGCAACAAATACTATTTTGATCAGGCTTGCCGGGTTAAAGCGATAGAA
ACCGCTTCAAAGCTGGGACTCGATAAGATGCTCAGTATCAACTTTCTGCCCAATGCAGTGTATAACCCTGAGACCTGTAT
CCGCGCGACGATTGAAGCCGCCGACATGTATGGGTTTGATATCCACAAAATCATGTTCGAAGTGACAGAGGGCGAACAAA
TTGTCGATCGGGCGAAATTAGTCCATATCTTCGAGAGTTATGCTAAACGTGGTTTTATCACCGCAATCGATGATTTTGGC
AGTGGTTTTGCTGATTTAAGTTGGTTGGATGCCCTGCGTCCCCAAGTGCTAAAACTCGATCTGACTTTGATCCGTGATAT
CGATGTCGATAAAGAAAAACAGCAGGCAGTGGATGAAATACTGCGTATTTGCCTAGAACTTAATACAAGAGTCTTGGCCG
AAGGCGTTGAGAGCGAAGCCGAACTGAATTATCTCGCTGGAATCGGGATTGAGTATTTTCAGGGCTATTACTTTGCTAAA
CCTAAGCTCGAACATTTAACTACCTACGAAGAGCTGAACTTTCATGCACCATAG

Upstream 100 bases:

>100_bases
TTTTCTATATTCCAGATTTTATTAACATGATGAACAGTAAAAACTGGCAAATTTAACCATTTATCCTCACACTAGTAAGG
TGATGTTAAGGAGGCCGTTT

Downstream 100 bases:

>100_bases
CCGCGCTCGTTCAACATCTTAAACTACTTGGTTATTCTTCTGCTTTGTGCGTTTACCATCGAATCTAAAATATTTAATTA
ACAGACACTTGCCTGCACGT

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MDFPARIKQQTCMDCLSGKSLGFEIRMAFQPIIHWPSREISGYEALVRGPEGEGAAWVFARINDSNKYYFDQACRVKAIE
TASKLGLDKMLSINFLPNAVYNPETCIRATIEAADMYGFDIHKIMFEVTEGEQIVDRAKLVHIFESYAKRGFITAIDDFG
SGFADLSWLDALRPQVLKLDLTLIRDIDVDKEKQQAVDEILRICLELNTRVLAEGVESEAELNYLAGIGIEYFQGYYFAK
PKLEHLTTYEELNFHAP

Sequences:

>Translated_257_residues
MDFPARIKQQTCMDCLSGKSLGFEIRMAFQPIIHWPSREISGYEALVRGPEGEGAAWVFARINDSNKYYFDQACRVKAIE
TASKLGLDKMLSINFLPNAVYNPETCIRATIEAADMYGFDIHKIMFEVTEGEQIVDRAKLVHIFESYAKRGFITAIDDFG
SGFADLSWLDALRPQVLKLDLTLIRDIDVDKEKQQAVDEILRICLELNTRVLAEGVESEAELNYLAGIGIEYFQGYYFAK
PKLEHLTTYEELNFHAP
>Mature_257_residues
MDFPARIKQQTCMDCLSGKSLGFEIRMAFQPIIHWPSREISGYEALVRGPEGEGAAWVFARINDSNKYYFDQACRVKAIE
TASKLGLDKMLSINFLPNAVYNPETCIRATIEAADMYGFDIHKIMFEVTEGEQIVDRAKLVHIFESYAKRGFITAIDDFG
SGFADLSWLDALRPQVLKLDLTLIRDIDVDKEKQQAVDEILRICLELNTRVLAEGVESEAELNYLAGIGIEYFQGYYFAK
PKLEHLTTYEELNFHAP

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=222, Percent_Identity=31.981981981982, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790496, Length=224, Percent_Identity=29.0178571428571, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI87081845, Length=229, Percent_Identity=29.6943231441048, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081980, Length=134, Percent_Identity=34.3283582089552, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI226510982, Length=227, Percent_Identity=26.8722466960352, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081921, Length=237, Percent_Identity=25.7383966244726, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786507, Length=134, Percent_Identity=27.6119402985075, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=156, Percent_Identity=30.1282051282051, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788502, Length=227, Percent_Identity=25.1101321585903, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87082096, Length=151, Percent_Identity=27.1523178807947, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081743, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788849, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29242; Mature: 29242

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFPARIKQQTCMDCLSGKSLGFEIRMAFQPIIHWPSREISGYEALVRGPEGEGAAWVFA
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCCCCCHHEEECCCCCCCEEEEE
RINDSNKYYFDQACRVKAIETASKLGLDKMLSINFLPNAVYNPETCIRATIEAADMYGFD
EEECCCCEEECHHHHHHHHHHHHHHCHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCH
IHKIMFEVTEGEQIVDRAKLVHIFESYAKRGFITAIDDFGSGFADLSWLDALRPQVLKLD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHCHHHEEEH
LTLIRDIDVDKEKQQAVDEILRICLELNTRVLAEGVESEAELNYLAGIGIEYFQGYYFAK
HHHEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCHHHHCCEEECC
PKLEHLTTYEELNFHAP
CCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDFPARIKQQTCMDCLSGKSLGFEIRMAFQPIIHWPSREISGYEALVRGPEGEGAAWVFA
CCCCHHHHHHHHHHHHCCCCCCEEEEEEEHHHHCCCCCCCCCCHHEEECCCCCCCEEEEE
RINDSNKYYFDQACRVKAIETASKLGLDKMLSINFLPNAVYNPETCIRATIEAADMYGFD
EEECCCCEEECHHHHHHHHHHHHHHCHHHHEEEECCCCCCCCCHHHHHHHHHHHHHCCCH
IHKIMFEVTEGEQIVDRAKLVHIFESYAKRGFITAIDDFGSGFADLSWLDALRPQVLKLD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCHHHHHHHHHHCHHHEEEH
LTLIRDIDVDKEKQQAVDEILRICLELNTRVLAEGVESEAELNYLAGIGIEYFQGYYFAK
HHHEECCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHHHHHHCCHHHHCCEEECC
PKLEHLTTYEELNFHAP
CCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]