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The map label for this gene is psrB [H]

Identifier: 126175973

GI number: 126175973

Start: 4437649

End: 4438215

Strand: Reverse

Name: psrB [H]

Synonym: Sbal_3780

Alternate gene names: 126175973

Gene position: 4438215-4437649 (Counterclockwise)

Preceding gene: 126175974

Following gene: 126175972

Centisome position: 86.56

GC content: 50.79

Gene sequence:

>567_bases
ATGAATAAACGATATGTAATGGTGCACGACGAGAATAAGTGCATCGGCTGCCAAGCTTGTAACGTGGCGTGTCGCAGTGA
AAATGGCGTACCCGAAGGCGTTACTCGTCTGCAAGTGCGTGTGGAAGGCCCCTTTGGTGAGATGCCCAATCTGCACTTCA
AATACCACAGGGTATCTTGCCAGCAATGTGAAGATGCGCCCTGCGTTAAGGTTTGCCCAACGGGCGCGGCCTATGTGGGT
GAAGACGGCATTATCTCTATCCATACCGAGAAGTGTGTCGGCTGTATGTACTGTGTGGCAGCGTGCCCCTACAAAGTGCG
CTTTATGAACCCAGAAACCAAGGCGGCGGATAAGTGTAACTTCTGTAAAGACAGTCGTTTAGCGCGGGGCGAAGAGCCAG
CGTGTGTGACTGTGTGTCCAACCGATGCCTTAGTGTTTGGCGATGCTAATGATCCGCAGAGTGACGTGGCCATCATGCTC
AACACTAAAATCACTTACCAAGATAAAACCCATTTGGGCACTAAACCTAGGGTATATTGCGTTCCTACCAAAAGAGGGGG
GATTTGA

Upstream 100 bases:

>100_bases
CCACTGCGGTAACTTACTGCCGCACGTTACAGCTCCTGTGTGTGGCATGACAGTGCACACCACGGGCGTCACGCTGGCAA
AGCGCTAATCGGAGGCTTAA

Downstream 100 bases:

>100_bases
TTATGAACAATACTTGGGGCGACATGGCGCAATATGATCCTGTCACTTGGCACTGGGTCATCGCCGTTTACCTGTTTATG
GCGGGGTTATCCGCGGGCAG

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Sulfur reductase chain B [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MNKRYVMVHDENKCIGCQACNVACRSENGVPEGVTRLQVRVEGPFGEMPNLHFKYHRVSCQQCEDAPCVKVCPTGAAYVG
EDGIISIHTEKCVGCMYCVAACPYKVRFMNPETKAADKCNFCKDSRLARGEEPACVTVCPTDALVFGDANDPQSDVAIML
NTKITYQDKTHLGTKPRVYCVPTKRGGI

Sequences:

>Translated_188_residues
MNKRYVMVHDENKCIGCQACNVACRSENGVPEGVTRLQVRVEGPFGEMPNLHFKYHRVSCQQCEDAPCVKVCPTGAAYVG
EDGIISIHTEKCVGCMYCVAACPYKVRFMNPETKAADKCNFCKDSRLARGEEPACVTVCPTDALVFGDANDPQSDVAIML
NTKITYQDKTHLGTKPRVYCVPTKRGGI
>Mature_188_residues
MNKRYVMVHDENKCIGCQACNVACRSENGVPEGVTRLQVRVEGPFGEMPNLHFKYHRVSCQQCEDAPCVKVCPTGAAYVG
EDGIISIHTEKCVGCMYCVAACPYKVRFMNPETKAADKCNFCKDSRLARGEEPACVTVCPTDALVFGDANDPQSDVAIML
NTKITYQDKTHLGTKPRVYCVPTKRGGI

Specific function: Component of the phosphorylative electron transport system with polysulfide as the terminal acceptor [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=186, Percent_Identity=53.2258064516129, Blast_Score=210, Evalue=4e-56,
Organism=Escherichia coli, GI226510944, Length=177, Percent_Identity=45.7627118644068, Blast_Score=170, Evalue=4e-44,
Organism=Escherichia coli, GI1787122, Length=193, Percent_Identity=32.6424870466321, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787872, Length=154, Percent_Identity=36.3636363636364, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787749, Length=154, Percent_Identity=35.7142857142857, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1790326, Length=154, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1789067, Length=170, Percent_Identity=33.5294117647059, Blast_Score=80, Evalue=8e-17,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=34.4827586206897, Blast_Score=78, Evalue=4e-16,
Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=35.4838709677419, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789370, Length=209, Percent_Identity=28.7081339712919, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=35.1351351351351, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787963, Length=156, Percent_Identity=26.9230769230769, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI87082180, Length=178, Percent_Identity=33.1460674157303, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787740, Length=96, Percent_Identity=35.4166666666667, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787478, Length=96, Percent_Identity=34.375, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 20678; Mature: 20678

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
12.2 %Cys+Met (Translated Protein)
9.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
12.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRYVMVHDENKCIGCQACNVACRSENGVPEGVTRLQVRVEGPFGEMPNLHFKYHRVSC
CCCCEEEEECCCCEEEEEECCEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCC
QQCEDAPCVKVCPTGAAYVGEDGIISIHTEKCVGCMYCVAACPYKVRFMNPETKAADKCN
CCCCCCCEEEECCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
FCKDSRLARGEEPACVTVCPTDALVFGDANDPQSDVAIMLNTKITYQDKTHLGTKPRVYC
CCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEECEEEECCCCCCCCCCEEEE
VPTKRGGI
EECCCCCC
>Mature Secondary Structure
MNKRYVMVHDENKCIGCQACNVACRSENGVPEGVTRLQVRVEGPFGEMPNLHFKYHRVSC
CCCCEEEEECCCCEEEEEECCEEEECCCCCCCCEEEEEEEEECCCCCCCCCEEEEEECCC
QQCEDAPCVKVCPTGAAYVGEDGIISIHTEKCVGCMYCVAACPYKVRFMNPETKAADKCN
CCCCCCCEEEECCCCCEEECCCCEEEEEHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCC
FCKDSRLARGEEPACVTVCPTDALVFGDANDPQSDVAIMLNTKITYQDKTHLGTKPRVYC
CCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEECEEEECCCCCCCCCCEEEE
VPTKRGGI
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1597189; 14500908 [H]