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The map label for this gene is ansB [H]

Identifier: 126175939

GI number: 126175939

Start: 4393461

End: 4394531

Strand: Reverse

Name: ansB [H]

Synonym: Sbal_3745

Alternate gene names: 126175939

Gene position: 4394531-4393461 (Counterclockwise)

Preceding gene: 126175947

Following gene: 126175937

Centisome position: 85.71

GC content: 48.46

Gene sequence:

>1071_bases
GTGTCGCAGTTATTATCTGAATTCTGGCGTAAGCTCTTGCTCTCGGTCACCTTAGGATTAGTTCTCTCCTCTGCGGCGCA
GGCGCTTCCACAGGTACGGATCATTGCCACGGGTGGCACTATTGCCGGAGTAGGGCAATCGGCCACTGAGTCTAACTATC
AGGCTGGTGAAGTGGGCATTAGTAGCTTAATTGCGGCTGTGCCTGAAATGAAACAGCTTGCCGATATTCAAGGTGAGCAA
CTGGTTAAAATCGGTTCGCAGGACATGAATGATAAAGTTTGGCTAGCGTTAGCCAAGCGCATCAATCTGTTACTGGCCGA
TAAAGAGGTCGACGGTATCGTCATCACCCACGGCACCGACACCTTAGAAGAAACCGCTTATTTTCTAAACCTCACCATCA
AGAGCGATAAACCTGTGGTATTGGTTGGCGCTATGCGTCCATCAACCGCCATGAGCGCCGATGGTCCAATGAATCTGTAT
AACGCCGTCGCAACCGCCGCGAACCTTGAATCTAAAGGTCGAGGTGTATTAGTGGTAATGAACGATACCGTATTGAACGC
CCGCGACGTTACTAAGACGAATACCACAGGTGTGCATACCTTTGCCGCGCCTAACTTTGGCCCGCTTGGCGTGATCCATA
ACAGCAAGATTGAGTACATGGGTAATACTGAGCGTCAACATACTACGCAAACGCCTTTTGATGTCAGCAAACTCGACAAG
TTACCGCAGGTAGGCATAGTGTATAGCTACGCCAATGCCAGTGATTTACCAGCCAAAGCCATGGTTGAAGCGGGTTTTGA
TGGCATAGTCAGTGCCGGTGTGGGTAACGGTAATATCTACCACTCTATATTCGATGTTTTAGCTAAAGCGAGTCAAAAAG
GCACCTTAGTCGTTCGCTCTAGCCGAGTGCCCACAGGTGCAACGACGCTCGATGCTGAGATAGATGATGCTAAATACGGT
TTTATTGCAGCAGGTAATCTTAACCCGCAAAAAGCCCGTATTTTGTTGATGCTATCGTTAACGAAAAGCAAAGATCCACA
GCAGATCCAACGTTGGTTTCAACAGTTTTAG

Upstream 100 bases:

>100_bases
CTTCGGTCAACTGTTGATAATTCATGGTGATACTCTTTTACTTTGGCGAGATAAAGCGTACCACTTTTCAGCAGTTGGCT
TCTTCTACACCTCGATTAGA

Downstream 100 bases:

>100_bases
GATCTCAGTTGAGTGGTTAATACTCAATAAGATTGATGTTTGCTAGATAAGCCAGCGGCAACGTCCGACTGGCTTTTTTA
TCGGCAGAGTTTGAGGTCTT

Product: L-asparaginase, type II

Products: NA

Alternate protein names: L-asparaginase II; L-ASNase II; L-asparagine amidohydrolase II; Colaspase [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSQLLSEFWRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQ
LVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLY
NAVATAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDK
LPQVGIVYSYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYG
FIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF

Sequences:

>Translated_356_residues
MSQLLSEFWRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQ
LVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLY
NAVATAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDK
LPQVGIVYSYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYG
FIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
>Mature_355_residues
SQLLSEFWRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGISSLIAAVPEMKQLADIQGEQL
VKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTDTLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYN
AVATAANLESKGRGVLVVMNDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDKL
PQVGIVYSYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRSSRVPTGATTLDAEIDDAKYGF
IAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=350, Percent_Identity=68.2857142857143, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1788065, Length=343, Percent_Identity=26.2390670553936, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI193207372, Length=279, Percent_Identity=25.089605734767, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558280, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17558278, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193207374, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=39.7014925373134, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=39.7014925373134, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=39.7014925373134, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=39.7014925373134, Blast_Score=204, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320527, Length=345, Percent_Identity=37.3913043478261, Blast_Score=192, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24645530, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 38047; Mature: 37916

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS00445 FGGY_KINASES_2 ; PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLSEFWRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCHHHHH
SSLIAAVPEMKQLADIQGEQLVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTD
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEECCCC
TLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVATAANLESKGRGVLVVM
CHHCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
NDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDK
CCCEECCCCCCCCCCCCEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCCCCCHHHHHC
LPQVGIVYSYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRS
CCCCEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
SRVPTGATTLDAEIDDAKYGFIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
CCCCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SQLLSEFWRKLLLSVTLGLVLSSAAQALPQVRIIATGGTIAGVGQSATESNYQAGEVGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCCHHHHH
SSLIAAVPEMKQLADIQGEQLVKIGSQDMNDKVWLALAKRINLLLADKEVDGIVITHGTD
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHEEEEECCCCCEEEEECCCC
TLEETAYFLNLTIKSDKPVVLVGAMRPSTAMSADGPMNLYNAVATAANLESKGRGVLVVM
CHHCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
NDTVLNARDVTKTNTTGVHTFAAPNFGPLGVIHNSKIEYMGNTERQHTTQTPFDVSKLDK
CCCEECCCCCCCCCCCCEEEEECCCCCCEEEEECCEEEECCCCCCCCCCCCCCCHHHHHC
LPQVGIVYSYANASDLPAKAMVEAGFDGIVSAGVGNGNIYHSIFDVLAKASQKGTLVVRS
CCCCEEEEEECCCCCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEE
SRVPTGATTLDAEIDDAKYGFIAAGNLNPQKARILLMLSLTKSKDPQQIQRWFQQF
CCCCCCCEEECCCCCCCCCCEEEECCCCCCCEEEEEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2407723; 2144836; 9278503; 6766894; 387570; 321449; 4561256; 1906013; 1521538; 1287659; 8434007; 8706862 [H]