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The map label for this gene is mlaE [H]

Identifier: 126175856

GI number: 126175856

Start: 4282178

End: 4282963

Strand: Reverse

Name: mlaE [H]

Synonym: Sbal_3661

Alternate gene names: 126175856

Gene position: 4282963-4282178 (Counterclockwise)

Preceding gene: 126175857

Following gene: 126175855

Centisome position: 83.53

GC content: 50.76

Gene sequence:

>786_bases
GTGAAGTTAATAGACAGTATTGCCAACTTAGGCCGCAGCGCGATTGAGTTAGTGCTCGGTTTTGGCCGCGCAGGGCTCAT
GTTGTGGGGGGCGATTTTTCGTTGGCCACGCGTGCGCAAAGGCTTTCCATTGTTAGTGCGCCAGATTTATGTAGTCGGCG
TGCAATCTATGGTGATTATTTTAGTATCAGGGCTCTTTATCGGCATGGTGTTGGCGCTGCAAGGCTACAACATCTTAGTT
GGCTTCGGCACCGAAGAAAGCTTAGGCCCTATGGTGGCGCTCAGTTTACTGCGTGAGTTAGGGCCCGTGGTTACCGCACT
TTTGTTTGCGGGACGTGCGGGTTCAGCCTTAACCGCTGAAATTGGTTTAATGAAAAGTACTGAGCAGCTCTCGAGTTTAG
AGATGATGGCTATCGACCCATTAAGGCAGATTATTGCACCACGCTTTTGGGCTGGGGTGATCAGTATGCCATTGCTGGCG
TTAATGTTTACCGCTGTGGGTATTTACGGTGGTCACATAGTAGGCGTGGAATGGAAGGGTATCGACAGCGGGGCGTTTTG
GTCGATTCTGCAGGCGTCGGTCGAATGGCGTCAGGACATAGTTAACTGCTTGATCAAGAGTGGTGTGTTTGCCGTAGTGG
TGACTTGGATTGCGTTGTATCGCGGTTATCAAGTGATCCCAAATCCGGAAGGCATCAGCCGTGCAACGACTCAGACAGTG
GTTCAGTCGAGCCTCGCTGTATTGGCATTAGATTTTTTACTGACAGCCCTGATGTTTGGCCGCTAA

Upstream 100 bases:

>100_bases
AGCTGAAACAGTTTATTCAAGGCGCACCGGATGGCCCTGTACCGTTCCATTATCCGGCGAATGATTACCAACAAGAATTA
ATGGCAAAAGGAGTTGGCAA

Downstream 100 bases:

>100_bases
CGGAACACTGCGGATTTCGACACAGGTAAGATGGATTAACAGAGTGGTAGCTAAACATGTTGACACGAAAAATTGAATTA
TTAGTGGGTGTGTTTCTTTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKLIDSIANLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR

Sequences:

>Translated_261_residues
MKLIDSIANLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR
>Mature_261_residues
MKLIDSIANLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVIILVSGLFIGMVLALQGYNILV
GFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAEIGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLA
LMFTAVGIYGGHIVGVEWKGIDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
VQSSLAVLALDFLLTALMFGR

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=259, Percent_Identity=68.7258687258687, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28241; Mature: 28241

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIDSIANLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLIDSIANLGRSAIELVLGFGRAGLMLWGAIFRWPRVRKGFPLLVRQIYVVGVQSMVII
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LVSGLFIGMVLALQGYNILVGFGTEESLGPMVALSLLRELGPVVTALLFAGRAGSALTAE
HHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
IGLMKSTEQLSSLEMMAIDPLRQIIAPRFWAGVISMPLLALMFTAVGIYGGHIVGVEWKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
IDSGAFWSILQASVEWRQDIVNCLIKSGVFAVVVTWIALYRGYQVIPNPEGISRATTQTV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
VQSSLAVLALDFLLTALMFGR
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]