Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 126175841

GI number: 126175841

Start: 4268140

End: 4268880

Strand: Reverse

Name: rlmB [H]

Synonym: Sbal_3646

Alternate gene names: 126175841

Gene position: 4268880-4268140 (Counterclockwise)

Preceding gene: 126175842

Following gene: 126175838

Centisome position: 83.26

GC content: 49.93

Gene sequence:

>741_bases
ATGAAAAAACAAGATATTATTTTTGGGATCCACGCAGTTGAAGCCCTGTTAAAACACAGCCCTGAGCGCATTATCGAGTT
ATGGCTGTTACAAGGCCGTGACGATGAACGCTTAACTGTGCTGGCCAATCAGGCCAAAGCCTTTGGCACTACGATTCAAG
TCGCTTCTCGCAAAGCCTTGGATGACAAAGCGGAAAGCAGCCAACATCAAGGTATAGTCGCACGCGTTAAAGCGGCGAAA
GTGTTGAACGAGCGAGATTTAGATGTGCTGCTTGCTAACACTGAGCAACCATTTCTGCTGATTTTGGATGGCGTGACGGA
TCCCCATAACTTAGGCGCTTGCCTACGTAATGCTGATGCGGCAGGCGTGCAAGGTATTATCGTGCCTAAAGATAACTCAG
TCGGCTTAACTGCGACTGTGAGTAAAGTGGCATGTGGCGCGGCGGAAACTGTGCCTTTATTCCAAGTGACTAATCTTGCG
CGCACTATGCGTCACCTGCAGGATAAAGGTGTATGGATTGTGGGTACGGCGGGCGAGGCAGATTGCGAGCTTTATCAAGC
CGATCTTAAAGGGCCACTGGCGATTGCCATGGGTGCGGAAGATAAAGGTTTACGCCGTTTAAGCCGTGAGTCTTGCGATT
CACTGATCTCTATCCCTATGGCCGGTAGTGTGTCTAGCTTGAACGTATCGGTTGCGACCGGTATTTGCTTATTTGAAGCT
GTGCGTCAGCGCCGCAGCTAA

Upstream 100 bases:

>100_bases
CAAACCTAAGGCTAAAGCGGGCACTAAAGCGAAAAAGACAGCAGTAAAAGATCCGGCTAAAAAGCCAAAAGCCGCTAAGC
GGAGCACGAGAAAGAAATAA

Downstream 100 bases:

>100_bases
TACGCTCATGTTTGCCTGATATCGCATATGCGGTCAGGCTTGCTGTAACGACTTATTGCAGTCACTATTTGTTGTAGTTA
GCATTAGCAGTAATAAAAGA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNERDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Sequences:

>Translated_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNERDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS
>Mature_246_residues
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKALDDKAESSQHQGIVARVKAAK
VLNERDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADAAGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLA
RTMRHLQDKGVWIVGTAGEADCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
VRQRRS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=28.5171102661597, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1790623, Length=240, Percent_Identity=65.4166666666667, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=27.4590163934426, Blast_Score=91, Evalue=5e-20,
Organism=Escherichia coli, GI1790083, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=258, Percent_Identity=27.1317829457364, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=165, Percent_Identity=33.9393939393939, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26415; Mature: 26415

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKAL
CCCCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCHHHHHHHHHH
DDKAESSQHQGIVARVKAAKVLNERDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADA
CCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLARTMRHLQDKGVWIVGTAGEA
CCCEEEEEECCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEHHHHHHHHHHH
VRQRRS
HHHHCC
>Mature Secondary Structure
MKKQDIIFGIHAVEALLKHSPERIIELWLLQGRDDERLTVLANQAKAFGTTIQVASRKAL
CCCCCEEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCHHHHHHHHHH
DDKAESSQHQGIVARVKAAKVLNERDLDVLLANTEQPFLLILDGVTDPHNLGACLRNADA
CCHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEECCCCCHHHHHHHHCCCC
AGVQGIIVPKDNSVGLTATVSKVACGAAETVPLFQVTNLARTMRHLQDKGVWIVGTAGEA
CCCEEEEEECCCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCC
DCELYQADLKGPLAIAMGAEDKGLRRLSRESCDSLISIPMAGSVSSLNVSVATGICLFEA
CHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCCEEHHHHHHHHHHH
VRQRRS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]