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The map label for this gene is rpfG [H]

Identifier: 126175701

GI number: 126175701

Start: 4104237

End: 4105253

Strand: Reverse

Name: rpfG [H]

Synonym: Sbal_3504

Alternate gene names: 126175701

Gene position: 4105253-4104237 (Counterclockwise)

Preceding gene: 126175702

Following gene: 126175698

Centisome position: 80.07

GC content: 48.57

Gene sequence:

>1017_bases
ATGGAAAAGGCAACAATATTAGTCGTCGATGACACACCGGAGAATATCGATATTTTGATCGGTATTTTGGGTGATGACTA
TAAAGTCAAAGTGGCGATCGATGGGCCAAGGGCGTTAGCCTTAGTGGCAAAGTCGCGGCCCGACTTAATTCTGCTTGATG
TGATGATGCCGGGAATGAATGGCTATGAGGTATGCAAGCTGCTAAAGCAAGATCCGCTGACCAGTCATATTCCAATCATT
TTTGTGACTGCTTTATCTGAATCCTCCGACGAAGCCCAAGGGTTTGCTTTGGGCGCAGTGGATTACATCACTAAGCCCGT
GAGTGCGCCTGTGGTTAAGGCGCGAGTGAAAACCCATCTTGCGCTCTATGATCAAAAACGCCTACTCGAACAACAAGTTA
AAATCCGTACCCACGAGTTAGAAGAAACCCGTTTTGAGATCATCCGCCGCCTCGGCCGCGCCGCCGAATATAAGGACAAC
GAGACAGGTCTACATGTGGTACGCATGAGCCACTACGCCCGAATATTGGCGATAAAGCTTGGTTTACCTAGCACTTTCTG
CGATTTAATTTTTAATGCGGCGCCGATGCACGATATCGGTAAAATCGGCACCCCGGATGCTGTGCTGAAAAAACCGGGTA
AGCTTGATGCCGAGGAGTGGCGCATCATGCAGCAACATGCCGCTATCGGCGCCGAAATTATCGGTGAACACAGCGATCCG
TTATTGCAGATGGCCCGTCGTATCGCCTTAACTCACCATGAGAAGTGGGATGGTTCAGGTTACCCTAATGGCTTGGTGGG
AGAGGATATTCCCCTTGAAGGGCGTATCACCGCGATTGCCGATGTGTTCGATGCCTTGACTTCGCAAAGGCCCTATAAAC
AGGCTTGGACTATTGAAGCGACGATGGAGTTATTCGATAATCAAGCAGGAAAACATTTTGATCCTAAGCTGGTGGAAATG
TTTAAAGAAATCCTGCCTGAGGCGATCGCCATTCGAGATACTTATTTGGAAAACTGA

Upstream 100 bases:

>100_bases
TACCAGTTTGATGAGGCGATCGACTTGATTGATGATGTCATTGCTCAGTTAGGATAAAAAAGTAACACACTGAATTCAAA
CGCATGAAGTGGAGTTGTTA

Downstream 100 bases:

>100_bases
TGCTCGGGTGCTCGGGTGCTCGGGTGCTCGGGTGCTCGGGTGTTTGAGGGGGTGAGTACAAGATCAGCTAAAGGCGCCAT
TGGCGCCTTTATTCTGTTTA

Product: response regulator receiver modulated metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MEKATILVVDDTPENIDILIGILGDDYKVKVAIDGPRALALVAKSRPDLILLDVMMPGMNGYEVCKLLKQDPLTSHIPII
FVTALSESSDEAQGFALGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKIRTHELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARILAIKLGLPSTFCDLIFNAAPMHDIGKIGTPDAVLKKPGKLDAEEWRIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLVGEDIPLEGRITAIADVFDALTSQRPYKQAWTIEATMELFDNQAGKHFDPKLVEM
FKEILPEAIAIRDTYLEN

Sequences:

>Translated_338_residues
MEKATILVVDDTPENIDILIGILGDDYKVKVAIDGPRALALVAKSRPDLILLDVMMPGMNGYEVCKLLKQDPLTSHIPII
FVTALSESSDEAQGFALGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKIRTHELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARILAIKLGLPSTFCDLIFNAAPMHDIGKIGTPDAVLKKPGKLDAEEWRIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLVGEDIPLEGRITAIADVFDALTSQRPYKQAWTIEATMELFDNQAGKHFDPKLVEM
FKEILPEAIAIRDTYLEN
>Mature_338_residues
MEKATILVVDDTPENIDILIGILGDDYKVKVAIDGPRALALVAKSRPDLILLDVMMPGMNGYEVCKLLKQDPLTSHIPII
FVTALSESSDEAQGFALGAVDYITKPVSAPVVKARVKTHLALYDQKRLLEQQVKIRTHELEETRFEIIRRLGRAAEYKDN
ETGLHVVRMSHYARILAIKLGLPSTFCDLIFNAAPMHDIGKIGTPDAVLKKPGKLDAEEWRIMQQHAAIGAEIIGEHSDP
LLQMARRIALTHHEKWDGSGYPNGLVGEDIPLEGRITAIADVFDALTSQRPYKQAWTIEATMELFDNQAGKHFDPKLVEM
FKEILPEAIAIRDTYLEN

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=142, Percent_Identity=39.4366197183099, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=35.042735042735, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790860, Length=121, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693157, Length=122, Percent_Identity=36.8852459016393, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=105, Percent_Identity=35.2380952380952, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082012, Length=94, Percent_Identity=35.1063829787234, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1786784, Length=116, Percent_Identity=31.8965517241379, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 37718; Mature: 37718

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKATILVVDDTPENIDILIGILGDDYKVKVAIDGPRALALVAKSRPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCC
GYEVCKLLKQDPLTSHIPIIFVTALSESSDEAQGFALGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKIRTHELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARILAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHEEEEEEEE
GLPSTFCDLIFNAAPMHDIGKIGTPDAVLKKPGKLDAEEWRIMQQHAAIGAEIIGEHSDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLQMARRIALTHHEKWDGSGYPNGLVGEDIPLEGRITAIADVFDALTSQRPYKQAWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEHHH
TMELFDNQAGKHFDPKLVEMFKEILPEAIAIRDTYLEN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKATILVVDDTPENIDILIGILGDDYKVKVAIDGPRALALVAKSRPDLILLDVMMPGMN
CCCEEEEEECCCCCCEEEEEEEECCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCCC
GYEVCKLLKQDPLTSHIPIIFVTALSESSDEAQGFALGAVDYITKPVSAPVVKARVKTHL
HHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHCCCCCHHHHHHHHHHH
ALYDQKRLLEQQVKIRTHELEETRFEIIRRLGRAAEYKDNETGLHVVRMSHYARILAIKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHEEEEEEEE
GLPSTFCDLIFNAAPMHDIGKIGTPDAVLKKPGKLDAEEWRIMQQHAAIGAEIIGEHSDP
CCCHHHHHHHHCCCCCHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCH
LLQMARRIALTHHEKWDGSGYPNGLVGEDIPLEGRITAIADVFDALTSQRPYKQAWTIEA
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHEEHHH
TMELFDNQAGKHFDPKLVEMFKEILPEAIAIRDTYLEN
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]