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The map label for this gene is proA [H]

Identifier: 126175521

GI number: 126175521

Start: 3888185

End: 3889462

Strand: Reverse

Name: proA [H]

Synonym: Sbal_3323

Alternate gene names: 126175521

Gene position: 3889462-3888185 (Counterclockwise)

Preceding gene: 126175522

Following gene: 126175517

Centisome position: 75.86

GC content: 49.06

Gene sequence:

>1278_bases
TTGAGTCAAATTAATCAAGATCAGTATTTACAGCAACTTGGTAATAATGCCAAACAGGCAAGTTATGCCTTAGCTAACTT
AACGGCGAGCCAAAAGGCCGGTTTGCTTGAAGCCATCGCCGATGCGCTGACAGAAAACGCCGCCGCCATTTTAGCCGCGA
ATGCTAAAGATGTTGCCGCCGCTAAAGCCGATGGATTAACGGATGCGATGATCGACAGATTGTTGCTCGATGAGGCGCGT
CTGGCGGGTATTATTGGCGATATTAGCGATGTGATCCGCCTTGCTGACCCAGTAGGTGAAGAATTTGGCAGTAAATTATT
AGATAACGGTTTAAGGCTGACTCGCCGCCGAGTACCACTTGGGGTTATCGGGGTGATTTACGAAGCTCGGCCTAATGTGA
CTGTTGATATCGCGGTACTTGCCCTTAAAACCGGTAATGCGGTGATCCTCCGTGGCGGTAAAGAAACTTTAGCATCGAAT
AAACTCATTAGTGACGTGATCCGCGGCGCGATTGTCGCTCAAGGTTTACCGATAGATGCGGTGCAATTTATTGATTCTTC
AGACAGAGCTTTAGTGACAGGTTTACTTAAGCTCGATCAATATGTCGACATGATAGTGCCCCGTGGCGGTCAAGCACTGC
AGCGCTTATGTGCTGAGCAGGCGACCATTCCGGTGATCTTAGGCGGCATTGGTATTTGTCATTTATATGTCGATAAAAAT
GCCAACCTTGAGCGTGCCTTAGCCGTGATCGCTAACGCCAAAGTGCAGCGTCCAACTGTATGTAATGCGCTTGATACCTT
GCTGGTGGATGAGCGCGTGGCGAGTACATTTGTGCCGCAAATTGCCGAGTATCTACATCGTTTAGGCGTGCGTTTTTCAG
TTTGTGACACAAGCTTTAGTCTGCTTGATGGTTTAGGGTTTGATGTGACGCTCGCCAAGGCTGAGGATTTTGGCATCGAA
TGGTTGTCGTTAACCTTAGGCATTAAAGTGGTGAGCGATATCGATGACGCCGTTAGTCATATTCGCCAATATTCTAGCGG
CCATTCGGAAGCGATTTTGACCGATGACATTCACGCTGCGGCGAACTTTATGAACGAAGTGAACTCGGCCGCCGTGTATG
TGAATGCCAGCACGCGTTTTACCGATGGCGGTCAGTTTGGTCTTGGCGCTGAAGTGGCTGTGAGCACGCAAAAACTGCAC
GCCCGCGGCCCAATGGGGCTTGAGGCATTAACCACATATAAGTGGTTAGCTTGGGGTGATTACACTAGCCGTGTTTAG

Upstream 100 bases:

>100_bases
AGGTAAGCATTCTGATGAGATCGAGTCTTTACTCGGTTACGATTATGGTGACGCAATCGTGCACCGCAACGACATGGTTG
TCTTATAAGCGAGGTCTGTT

Downstream 100 bases:

>100_bases
CGGTTAAAAACCATAAACTTAAAGCCGTTAACACACTGTGTTGACGGCTTTTTTGTTTGCAGTGTTTGATTGTAAGTGCC
AATTTGCAGAGTCTATTTAT

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDEAR
LAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASN
KLISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIE
WLSLTLGIKVVSDIDDAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRV

Sequences:

>Translated_425_residues
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDEAR
LAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASN
KLISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIE
WLSLTLGIKVVSDIDDAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
ARGPMGLEALTTYKWLAWGDYTSRV
>Mature_424_residues
SQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAAAKADGLTDAMIDRLLLDEARL
AGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPLGVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNK
LISDVIRGAIVAQGLPIDAVQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKNA
NLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFSLLDGLGFDVTLAKAEDFGIEW
LSLTLGIKVVSDIDDAVSHIRQYSSGHSEAILTDDIHAAANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHA
RGPMGLEALTTYKWLAWGDYTSRV

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=413, Percent_Identity=36.319612590799, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI21361368, Length=413, Percent_Identity=36.319612590799, Blast_Score=248, Evalue=5e-66,
Organism=Escherichia coli, GI1786438, Length=415, Percent_Identity=58.7951807228916, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=33.4939759036145, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=33.4939759036145, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324899, Length=431, Percent_Identity=37.8190255220418, Blast_Score=277, Evalue=2e-75,
Organism=Drosophila melanogaster, GI21357643, Length=410, Percent_Identity=36.5853658536585, Blast_Score=255, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 45293; Mature: 45162

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAA
CCCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKADGLTDAMIDRLLLDEARLAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNKLISDVIRGAIVAQGLPIDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFS
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHH
LLDGLGFDVTLAKAEDFGIEWLSLTLGIKVVSDIDDAVSHIRQYSSGHSEAILTDDIHAA
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
ANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHEEEECC
YTSRV
HHCCC
>Mature Secondary Structure 
SQINQDQYLQQLGNNAKQASYALANLTASQKAGLLEAIADALTENAAAILAANAKDVAA
CCCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHH
AKADGLTDAMIDRLLLDEARLAGIIGDISDVIRLADPVGEEFGSKLLDNGLRLTRRRVPL
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCE
GVIGVIYEARPNVTVDIAVLALKTGNAVILRGGKETLASNKLISDVIRGAIVAQGLPIDA
EEEEEEEECCCCCEEEEEEEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
VQFIDSSDRALVTGLLKLDQYVDMIVPRGGQALQRLCAEQATIPVILGGIGICHLYVDKN
HHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCEEEEEEECCC
ANLERALAVIANAKVQRPTVCNALDTLLVDERVASTFVPQIAEYLHRLGVRFSVCDTSFS
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHH
LLDGLGFDVTLAKAEDFGIEWLSLTLGIKVVSDIDDAVSHIRQYSSGHSEAILTDDIHAA
HHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHH
ANFMNEVNSAAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLEALTTYKWLAWGD
HHHHHHCCCEEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCHHHHHHHHEEEECC
YTSRV
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]