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The map label for this gene is mrdA [H]

Identifier: 126175475

GI number: 126175475

Start: 3836566

End: 3838422

Strand: Direct

Name: mrdA [H]

Synonym: Sbal_3275

Alternate gene names: 126175475

Gene position: 3836566-3838422 (Clockwise)

Preceding gene: 126175474

Following gene: 126175476

Centisome position: 74.83

GC content: 48.52

Gene sequence:

>1857_bases
GTGTCGCCAAAAAAGCGGATAACAATGCATGATCACGCCGCAGAGGCGTCTCTTTTTAAGCGCCGAGCGCTATTCACCTT
TTTTTGCGTTTTCGCCCTATTAAGCGTGTTAGTTACCAACCTTTATCATTTACAGGTTGAATCCTATAAGGATTATGAAA
CTCGCTCGAACGATAACCGTATCCGCGTTGTCCCAATTGCCCCGAGCCGTGGTCTCATTTACGATCGTAACGGCGTGCTA
TTAGCTGAAAACTTACCCTTTTATTCCCTCGATCTAGTTCCCGAAAAAATTAGCAACATGTCAGAGACCTTAGATGAGCT
CGGCAAGTTAATTGAGATCAGTGAGGATGAGCGCGAAACCTTTACCGAAGCGCTGAAATTTCATCGCCGCTTCAAACCAC
TGACGCTGAAAAACCAACTGACCGAAGAGCAAGTGGCGATTTTCAGTGTTAACCAACATAAATTTCCAGGCATTTCCGTA
GAAGCGGGCCTGAAGCGTAATTATCCCTACGGCGCGCAAATCACCCATGTACTGGGTTATGTCGGCAAGATTAACACCCG
AGATCGCGCCCAGTTAGAGCGCAGCGATCTGTGGAAAAACTATGCGGCTACCAAGGACATTGGTAAGCAAGGCATTGAAA
AATTTTATGAATCCCTACTGCATGGCAAACCGGGTCACTTAGAAGAAGAAGTGAACAACCGCGGCCGCGCGATTCGCACC
TTAAAAATTGTGCCGCCAGAGCCGGGGCAAGATATTTATCTGACATTAGATCTCAAGCTGCAGCAAAAAGCGGTTGAGCT
ACTCACAGGTCTGCGCGGTTCTATTGTTGCAATCGATCCCCGTGACGGTGGCGTTTTAGCTATGGTGTCGAGCCCAAGCT
ATGATCCAAACCAATTTGTGCAAGGGATTAATAACAAAGATTACAGCGCCTTACTCAACGATAAATCACGCCCCTTGATC
AACCGCGCGACCCAAGGCCAATATGCGCCCGCATCAACCATCAAGCCCCATCTCGCACTCTTAGGTTTAGATGAAAAAGT
CATTACCGAGAGCACCCGTGTGTGGGACCCAGGTTTTTGGCAAATTCCCGGTGTCGAACGTAAGTACCGTGACTGGAGAC
GCTGGGGACACGGCTGGGTTAACGTTTACAGTGCCATTACCGAATCCTGCGATACCTTCTTCTACGAACTCGCCTACAAA
GTTGGGGTAGACCATATTGCCCGCTTTATGGAGCAATTTGGCTTTGGTCAAAATACCGGAATCGATATCTTTGAAGAATC
TTCTGGCAACATGCCATCCAAAGAATGGAAACGCTTAAAGTACAATCAAGCTTGGTACATAGGCGATACCATTTCCGTCG
GTATTGGCCAAGGTTATTGGACTGCAACCCCATTACAACTGGCGAATGCCGTTGGGATCATGGCTAACAAAGGCCGTCGT
TTTCCGCCGCATCTGCTGAAGTCCATTAAAGACAATACGGCCAAGATTGACTCACCGATTAATGAGTTACCGCCGATTGA
GCTTAATAGTCCCCGTAACTGGAATATCATCAACGAAGCGATGCGCCAGACGGCGAATAAATCACGCTTCACCGATGCCA
CTTACACCGCCGCGATGAAAACCGGTACGGCGCAGGTTATTGGCGTAGCAGAAAATACCAAATACGATGCGAGTAAAATT
GCCGAACATTTCCGCGATAATGCCTTAGTTGTGGCCTATGCGCCCTTCGAAGATCCTAAAATCGTCCTCGCCGTCGTGAT
GGAAAACGCAGGTTGGGGCGGCGCAAATGCTGGCCCCGTCGCTCGCGCCATGCTCGATGAATATATGCTTAGAGATACTT
GGAAACCGCAACCATGA

Upstream 100 bases:

>100_bases
TGGAGCGTGAACACGAATCACCCCTACCATAGAGAATAGCGGGATCTTGATGTTAACGAGTGTCAACCACCCGTCATGGG
ATCAGCCGGAGATAGCATAA

Downstream 100 bases:

>100_bases
GTACCCATAGCCAATATCAAAACATCTGGCAACGCTTGCACATAGATTTGCCATTATTGCTCGGCCTATTGGCTGTGATG
GGCTTTGGCCTGTTCGTCAT

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSPKKRITMHDHAAEASLFKRRALFTFFCVFALLSVLVTNLYHLQVESYKDYETRSNDNRIRVVPIAPSRGLIYDRNGVL
LAENLPFYSLDLVPEKISNMSETLDELGKLIEISEDERETFTEALKFHRRFKPLTLKNQLTEEQVAIFSVNQHKFPGISV
EAGLKRNYPYGAQITHVLGYVGKINTRDRAQLERSDLWKNYAATKDIGKQGIEKFYESLLHGKPGHLEEEVNNRGRAIRT
LKIVPPEPGQDIYLTLDLKLQQKAVELLTGLRGSIVAIDPRDGGVLAMVSSPSYDPNQFVQGINNKDYSALLNDKSRPLI
NRATQGQYAPASTIKPHLALLGLDEKVITESTRVWDPGFWQIPGVERKYRDWRRWGHGWVNVYSAITESCDTFFYELAYK
VGVDHIARFMEQFGFGQNTGIDIFEESSGNMPSKEWKRLKYNQAWYIGDTISVGIGQGYWTATPLQLANAVGIMANKGRR
FPPHLLKSIKDNTAKIDSPINELPPIELNSPRNWNIINEAMRQTANKSRFTDATYTAAMKTGTAQVIGVAENTKYDASKI
AEHFRDNALVVAYAPFEDPKIVLAVVMENAGWGGANAGPVARAMLDEYMLRDTWKPQP

Sequences:

>Translated_618_residues
MSPKKRITMHDHAAEASLFKRRALFTFFCVFALLSVLVTNLYHLQVESYKDYETRSNDNRIRVVPIAPSRGLIYDRNGVL
LAENLPFYSLDLVPEKISNMSETLDELGKLIEISEDERETFTEALKFHRRFKPLTLKNQLTEEQVAIFSVNQHKFPGISV
EAGLKRNYPYGAQITHVLGYVGKINTRDRAQLERSDLWKNYAATKDIGKQGIEKFYESLLHGKPGHLEEEVNNRGRAIRT
LKIVPPEPGQDIYLTLDLKLQQKAVELLTGLRGSIVAIDPRDGGVLAMVSSPSYDPNQFVQGINNKDYSALLNDKSRPLI
NRATQGQYAPASTIKPHLALLGLDEKVITESTRVWDPGFWQIPGVERKYRDWRRWGHGWVNVYSAITESCDTFFYELAYK
VGVDHIARFMEQFGFGQNTGIDIFEESSGNMPSKEWKRLKYNQAWYIGDTISVGIGQGYWTATPLQLANAVGIMANKGRR
FPPHLLKSIKDNTAKIDSPINELPPIELNSPRNWNIINEAMRQTANKSRFTDATYTAAMKTGTAQVIGVAENTKYDASKI
AEHFRDNALVVAYAPFEDPKIVLAVVMENAGWGGANAGPVARAMLDEYMLRDTWKPQP
>Mature_617_residues
SPKKRITMHDHAAEASLFKRRALFTFFCVFALLSVLVTNLYHLQVESYKDYETRSNDNRIRVVPIAPSRGLIYDRNGVLL
AENLPFYSLDLVPEKISNMSETLDELGKLIEISEDERETFTEALKFHRRFKPLTLKNQLTEEQVAIFSVNQHKFPGISVE
AGLKRNYPYGAQITHVLGYVGKINTRDRAQLERSDLWKNYAATKDIGKQGIEKFYESLLHGKPGHLEEEVNNRGRAIRTL
KIVPPEPGQDIYLTLDLKLQQKAVELLTGLRGSIVAIDPRDGGVLAMVSSPSYDPNQFVQGINNKDYSALLNDKSRPLIN
RATQGQYAPASTIKPHLALLGLDEKVITESTRVWDPGFWQIPGVERKYRDWRRWGHGWVNVYSAITESCDTFFYELAYKV
GVDHIARFMEQFGFGQNTGIDIFEESSGNMPSKEWKRLKYNQAWYIGDTISVGIGQGYWTATPLQLANAVGIMANKGRRF
PPHLLKSIKDNTAKIDSPINELPPIELNSPRNWNIINEAMRQTANKSRFTDATYTAAMKTGTAQVIGVAENTKYDASKIA
EHFRDNALVVAYAPFEDPKIVLAVVMENAGWGGANAGPVARAMLDEYMLRDTWKPQP

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=611, Percent_Identity=50.4091653027823, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=600, Percent_Identity=24, Blast_Score=119, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69878; Mature: 69747

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKKRITMHDHAAEASLFKRRALFTFFCVFALLSVLVTNLYHLQVESYKDYETRSNDNR
CCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCE
IRVVPIAPSRGLIYDRNGVLLAENLPFYSLDLVPEKISNMSETLDELGKLIEISEDERET
EEEEEECCCCCEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCHHHHH
FTEALKFHRRFKPLTLKNQLTEEQVAIFSVNQHKFPGISVEAGLKRNYPYGAQITHVLGY
HHHHHHHHHHCCCCCHHHHCCHHHEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHH
VGKINTRDRAQLERSDLWKNYAATKDIGKQGIEKFYESLLHGKPGHLEEEVNNRGRAIRT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEE
LKIVPPEPGQDIYLTLDLKLQQKAVELLTGLRGSIVAIDPRDGGVLAMVSSPSYDPNQFV
EEEECCCCCCEEEEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCHHHHH
QGINNKDYSALLNDKSRPLINRATQGQYAPASTIKPHLALLGLDEKVITESTRVWDPGFW
HCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCCCE
QIPGVERKYRDWRRWGHGWVNVYSAITESCDTFFYELAYKVGVDHIARFMEQFGFGQNTG
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
IDIFEESSGNMPSKEWKRLKYNQAWYIGDTISVGIGQGYWTATPLQLANAVGIMANKGRR
CEEEECCCCCCCHHHHHHCCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
FPPHLLKSIKDNTAKIDSPINELPPIELNSPRNWNIINEAMRQTANKSRFTDATYTAAMK
CCHHHHHHHCCCCHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHC
TGTAQVIGVAENTKYDASKIAEHFRDNALVVAYAPFEDPKIVLAVVMENAGWGGANAGPV
CCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHH
ARAMLDEYMLRDTWKPQP
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPKKRITMHDHAAEASLFKRRALFTFFCVFALLSVLVTNLYHLQVESYKDYETRSNDNR
CCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCE
IRVVPIAPSRGLIYDRNGVLLAENLPFYSLDLVPEKISNMSETLDELGKLIEISEDERET
EEEEEECCCCCEEECCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHEECCCHHHHH
FTEALKFHRRFKPLTLKNQLTEEQVAIFSVNQHKFPGISVEAGLKRNYPYGAQITHVLGY
HHHHHHHHHHCCCCCHHHHCCHHHEEEEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHH
VGKINTRDRAQLERSDLWKNYAATKDIGKQGIEKFYESLLHGKPGHLEEEVNNRGRAIRT
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEE
LKIVPPEPGQDIYLTLDLKLQQKAVELLTGLRGSIVAIDPRDGGVLAMVSSPSYDPNQFV
EEEECCCCCCEEEEEEEEHHHHHHHHHHHCCCCCEEEECCCCCCEEEEECCCCCCHHHHH
QGINNKDYSALLNDKSRPLINRATQGQYAPASTIKPHLALLGLDEKVITESTRVWDPGFW
HCCCCCCHHHHHCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHCCCCCCCCCCE
QIPGVERKYRDWRRWGHGWVNVYSAITESCDTFFYELAYKVGVDHIARFMEQFGFGQNTG
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCC
IDIFEESSGNMPSKEWKRLKYNQAWYIGDTISVGIGQGYWTATPLQLANAVGIMANKGRR
CEEEECCCCCCCHHHHHHCCCCCEEEECCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCC
FPPHLLKSIKDNTAKIDSPINELPPIELNSPRNWNIINEAMRQTANKSRFTDATYTAAMK
CCHHHHHHHCCCCHHCCCCHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHC
TGTAQVIGVAENTKYDASKIAEHFRDNALVVAYAPFEDPKIVLAVVMENAGWGGANAGPV
CCCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCCCHH
ARAMLDEYMLRDTWKPQP
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]