Click here to switch to the map view.

The map label for this gene is miaB [H]

Identifier: 126175461

GI number: 126175461

Start: 3822879

End: 3824303

Strand: Direct

Name: miaB [H]

Synonym: Sbal_3261

Alternate gene names: 126175461

Gene position: 3822879-3824303 (Clockwise)

Preceding gene: 126175460

Following gene: 126175462

Centisome position: 74.56

GC content: 49.05

Gene sequence:

>1425_bases
ATGAGTAAGAAACTCCATATCAAGACTTGGGGCTGTCAAATGAATGAGTATGACTCATCCAAGATGGCTGATCTGTTAGG
CGAATACCAAGGTTATACCTTGACTGAAGAAGCGTCTGAGGCAGACATTCTCCTGCTGAATACTTGTTCAATCCGTGAAA
AAGCGCAGGAAAAAGTGTTCCATCAGCTAGGACGCTGGAAGACGCTTAAAGATAAAAATCCCAATTTGATTATTGGCGTA
GGCGGCTGCGTTGCTTCACAAGAAGGCAAAGCGATTAAAGACAGAGCCCAATGTGTCGACATTATTTTTGGACCACAAAC
GTTACACCGTCTGCCCGAGATGATCGAGCAAGTGCGCCGTGGTGAAAAAGCGGTTATCGATATCAGCTTCCCTGAAATCG
AAAAATTCGACCGTCTGCCAGAGCCCCGCGCCGAAGGCCCAACCGCGTTTGTGTCTATCATGGAAGGTTGCAGCAAATAC
TGCTCGTTCTGTGTGGTACCTTATACTCGCGGTGAAGAGGTCAGCCGTCCATCGGACGACATCATCCTTGAAATCGCCCA
ATTGGCTGAGCAAGGTGTGCGCGAAGTAAACCTGCTAGGTCAAAACGTGAATGCCTATCGCGGCGCAACTCACGATGGCG
CTATCTGTACGTTTGCTGAGTTACTGCGTTTCGTGGCAGCCATCGACGGTATTGACCGTATCCGCTTCACGACCAGTCAC
CCGATTGAATTTACACAAGATATTATCGACGTTTACGAAGATACCCCAGAGCTAGTGAGCTTCTTACACTTGCCAGTACA
ATCAGGTTCTGACCGTATTCTAACGGCGATGAAGCGTGGCCACATGGCGATTGAATATAAGTCAATCATCCGCCGCCTGC
GTAAAGCCCGTGAAGGCATTCAAATCAGCTCAGACTTTATCATTGGCTTCCCTGGCGAAACCAAAGAAGACTTTGCTGAT
ACCATTAAGTTGATTGAAGACATAGGTTTCGACCACAGCTTCAGCTTTATCTATAGCGCACGTCCGGGCACGCCAGCGGC
GGATTTACCCGATAACGTCGATATGGAAGAGAAAAAGCAACGTCTCGCGATTCTGCAAGACCGCATCACCCAGCAAGCCA
TGCGCTACAGCCGCCACATGATGGGCACAGTGCAACGTATTCTGGTTGAAGGTCCATCGGTGAAAAACCCAATGGAACTG
CGCGGCCGTACTGAGAACAACCGAGTGGTGAACTTCGAAGGTCAGCCAAAACACATTGGTACTTTCGTGGATGTCGAAAT
CGTCGACGTTTACACTAACTCACTGCGCGGTGTCTTTATCCGCGGCGAAGATGAAATGGATCTACGTCGTAGCCTGCGTC
CAGCTGAAATTTTGGCCAAGCGCAAGCAAGATGATGAGCTGGGTGTGACTCAATTTAAGCCATAA

Upstream 100 bases:

>100_bases
AAAGCCAACAGGTAAAAGTGAGGACAAAGCTGGTCAGCAGCCATAACAGCAGGTAAAATGTCGCGCTATTTTTATTGGTC
ATCTTAGGGCGACACAACTG

Downstream 100 bases:

>100_bases
GCTTTAAATCGGCTTAAACAGTACCTATATAGAAAAGGGCGACTTAGTCGCCCTTTTGCTTTCACGCACTTTTATTTAAG
TCCACCGAGGCAATTGCTAT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPNLIIGV
GGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRRGEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGIQISSDFIIGFPGETKEDFAD
TIKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMEL
RGRTENNRVVNFEGQPKHIGTFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP

Sequences:

>Translated_474_residues
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPNLIIGV
GGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRRGEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGIQISSDFIIGFPGETKEDFAD
TIKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMEL
RGRTENNRVVNFEGQPKHIGTFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP
>Mature_473_residues
SKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVFHQLGRWKTLKDKNPNLIIGVG
GCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRRGEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPSDDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSHP
IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGIQISSDFIIGFPGETKEDFADT
IKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQRLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELR
GRTENNRVVNFEGQPKHIGTFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=35.9832635983264, Blast_Score=283, Evalue=3e-76,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=35.9832635983264, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI93277076, Length=448, Percent_Identity=29.6875, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=75.5274261603376, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=26.2790697674419, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=32.2510822510823, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=314, Percent_Identity=28.0254777070064, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=36.2586605080831, Blast_Score=277, Evalue=9e-75,
Organism=Drosophila melanogaster, GI19922432, Length=466, Percent_Identity=27.8969957081545, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53675; Mature: 53544

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRR
HHHHCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHC
GEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD
CCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHH
IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHCCC
QISSDFIIGFPGETKEDFADTIKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQ
EEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCCCCC
TFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP
EEEEEEEEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHHHHHHHHHHH
HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRR
HHHHCCCCCCCCCCCEEEECCCEECCCCCCHHHHHHHHHHEEECHHHHHHHHHHHHHHHC
GEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD
CCEEEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHH
IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGI
CHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHHHHHHHHCCC
QISSDFIIGFPGETKEDFADTIKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQ
EEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHH
RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCEEEEECCCCCCCC
TFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP
EEEEEEEEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA