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The map label for this gene is prfC [H]

Identifier: 126175432

GI number: 126175432

Start: 3787733

End: 3789313

Strand: Reverse

Name: prfC [H]

Synonym: Sbal_3232

Alternate gene names: 126175432

Gene position: 3789313-3787733 (Counterclockwise)

Preceding gene: 126175433

Following gene: 126175431

Centisome position: 73.9

GC content: 47.31

Gene sequence:

>1581_bases
ATGTCAGGCAATAAAGTAGAGGTCGATAAACGTCGCACCTTCGCGATCATATCGCACCCCGACGCGGGTAAAACAACCAT
TACCGAAAAAGTGCTGTTATTCGGAAACGCTTTGCAAAAAGCAGGAACGGTTAAAGGCAAAAAATCGGGTCAGCATGCTA
AGTCTGACTGGATGGAAATGGAGAAGGACCGTGGTATTTCGATTACTACGTCTGTGATGCAGTTCCCCTATGGTGGCGCC
CTCGTTAACCTACTCGACACGCCTGGTCACGAAGACTTCTCTGAAGATACCTATCGTACACTGACCGCTGTTGACTCTTG
TCTGATGGTCATCGACTCTGCTAAAGGTGTTGAAGAGCGTACTATTAAGTTGATGGAAGTGACACGTCTGCGCGATACGC
CGATCGTGACTTTCATGAACAAACTCGACCGTGATATCCGTGATCCTATCGATCTGATGGATGAAGTCGAAAGCGTGCTG
AATATTGCCTGTGCACCTATTACTTGGCCTATTGGCAGCGGTAAAGAATTCAAAGGGATTTACCATATACTGCGTGATGA
AGTGGTCTTGTATCAAGGCGGTATGGGACACACAATTCAAGAGCGTCGTGTAATTAAAGGCATTAATAATCCTGATCTTG
AGAAAGCGATTGGCAGTTACGCTGCTGATTTGCGCGATGAGATGGAATTAGTGCGCGGCGCGTCGCATGAATTTGACCAT
GCAGCTTTCTTGAAAGGCGAGTTGACGCCAGTGTTCTTCGGTACAGCGTTAGGTAACTTTGGTGTGGACCATATTCTTGA
CGGCATCGTCGAGTGGGCGCCTAAGCCATTACCGCGCGAAAGCGATACACGCGTTATTATGCCTGATGAAGAAAAATTCA
CCGGTTTCGTGTTCAAGATCCAAGCTAACATGGATCCAAAACACCGTGACCGTGTCGCCTTTATGCGTGTGTGTTCTGGC
CGTTACGAGCAAGGCATGAAAATGCACCATGTGCGTATTGGTAAAGATGTTAACGTTAGCGATGCATTGACCTTTATGGC
GGGCGATCGTGAGCGTGCCGAGGAAGCTTACCCTGGTGATATCATCGGCTTGCATAACCATGGCACTATCCGTATTGGCG
ATACTTTTACCCAAGGCGAAAAATTCCGTTTCACTGGTGTGCCTAACTTCGCCCCTGAAATGTTCCGCCGTATTCGTCTG
CGCGATCCGCTTAAGCAAAAGCAGCTGTTAAAAGGCTTAGTACAGTTGTCTGAAGAAGGCGCAGTGCAAGTATTTCGCCC
AATCGATACTAACGATTTAATTGTTGGCGCCGTGGGTGTGCTACAGTTTGAAGTGGTTGTTGGTCGTCTTAAGAGCGAAT
ATAACGTTGAAGCCATTTACGAAGGTATTAGCGTTTCGACCGCTCGCTGGGTTTATTGTAAGGATGAGCGTAAGCTTGAA
GAGTTCCGCCGCAAGTGTAGCCAAAACTTAGCACTCGATGGTGGTGACAACTTAACCTATATTGCGCCAACTATGGTCAA
CCTGAATCTTTCGATGGAAAGGTATCCTGATATTGAGTTTGCAAAAACTCGCGAGCACTAA

Upstream 100 bases:

>100_bases
ACCCCCATCTGCCCGAGCTCTTCTCGGGCTTTGCCTAGCCAAGCTCGAACTGAGGCGCTATAATTTGCGCCTTTTTAGCG
ATACTAGTGCATAGGTTAAC

Downstream 100 bases:

>100_bases
TTAGGTTCGGTTTAGAATAGTTGTTGAAATGGCGGTGAATTCACCGCCATTTTTGTATCTAATGAAATCTAAAAACGGTT
CTGGATGTGAATGATGGCGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVL
NIACAPITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDH
AAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Sequences:

>Translated_526_residues
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGA
LVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVL
NIACAPITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDH
AAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSG
RYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRL
RDPLKQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
>Mature_525_residues
SGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGAL
VNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLN
IACAPITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHA
AFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKIQANMDPKHRDRVAFMRVCSGR
YEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLR
DPLKQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEE
FRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=492, Percent_Identity=27.6422764227642, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=25.7367387033399, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.2264631043257, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI25306287, Length=481, Percent_Identity=25.1559251559252, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI157426893, Length=180, Percent_Identity=27.7777777777778, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=77.9270633397313, Blast_Score=866, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=499, Percent_Identity=25.0501002004008, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.1282051282051, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=26.4462809917355, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=182, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.8518518518519, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17506493, Length=163, Percent_Identity=29.4478527607362, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=26.6666666666667, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=528, Percent_Identity=23.8636363636364, Blast_Score=120, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=155, Percent_Identity=29.6774193548387, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=155, Percent_Identity=29.6774193548387, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=22.7848101265823, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582462, Length=474, Percent_Identity=26.5822784810127, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI221458488, Length=501, Percent_Identity=23.3532934131737, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI78706572, Length=214, Percent_Identity=29.4392523364486, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59279; Mature: 59147

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEER
HHCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIACAPITWPIGSGKEFKGI
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
YHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDH
HHHHHCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKI
CEEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGD
EECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHHEEECCHHHHHHCCCCC
IIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
CEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHH
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SGNKVEVDKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEM
CCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
EKDRGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEER
HHCCCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHH
TIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIACAPITWPIGSGKEFKGI
HHHHHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
YHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDH
HHHHHCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AAFLKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDTRVIMPDEEKFTGFVFKI
CEEECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEE
QANMDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGD
EECCCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHHEEECCHHHHHHCCCCC
IIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEG
EEEECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
AVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLE
CEEEEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHH
EFRRKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA