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The map label for this gene is queA [H]

Identifier: 126174995

GI number: 126174995

Start: 3277205

End: 3278242

Strand: Reverse

Name: queA [H]

Synonym: Sbal_2788

Alternate gene names: 126174995

Gene position: 3278242-3277205 (Counterclockwise)

Preceding gene: 126174996

Following gene: 126174994

Centisome position: 63.94

GC content: 50.0

Gene sequence:

>1038_bases
ATGCGCGTTGCAGACTTTTCTTTCGATCTCCCCGATGAGCTGATCGCTCGTTATCCTATGGCGCAGCGTAATGCATCACG
TCTGTTAACCTTAGACGGCAATTCTGGCGCGTTAGGCGACAAACAGTTTACCGATCTGCTGGGGATGATTAATCCCGGCG
ATTTAATGGTGTTCAACAATACCCGAGTGATCCCCGCGCGTATGTTTGGCCAAAAGGCCAGCGGCGGCAAGTTAGAGATT
TTAGTTGAGCGTATGCTCGATGATAAGCGCATCCTCGCCCATGTTCGCAGCTCTAAATCCCCGAAAGTCGATTCGTTTAT
TCTGCTCGACGGTGGCTATCAAATGAAGATGGTCGCCCGTCACGATACTTTGTTTGAACTTGAGCTGTTATCCGAGTTGA
CCATTCTAGAGGTGCTCGAAGCCGTAGGGCACATGCCGCTACCGCCTTATATCGACAGACCCGATGAAGATGCTGACAAA
GAGCGCTATCAAACTGTGTATAACCAAAACCCAGGTGCCGTTGCTGCGCCAACGGCGGGGTTACATTTTGACGATGCCAT
GCTCGATGCATTAAAAGCCAAGGATGTGAATATCGCATTTGTGACTTTGCATGTGGGCGCGGGAACATTTCAGCCAGTGC
GTGTCGATACCATTCTTGAGCACAAGATGCATTCTGAGTGGGCCAATGTGCCGCAGGATGTGGTCGATTTAATCGCGCAA
ACGAAAGCCGCAGGTAAACGCGTAGTGGCTGTTGGTACAACATCTGTACGTTCCTTAGAAAGTGCGGCGCGCGCCAGCCA
AGGTGAACTCAAAGCCTTTAGCGGTGATACCGACATCTTTATTTATCCCGGCTATCAATTCCAAGTGGTCGATGCCATGG
TGACGAACTTCCACCTCCCTGAATCGACCCTCATCATGTTAGTCAGTGCATTTGCGGGTTTTGATCATGTTATGGCGGCG
TATCAACATGCTATCGCGCAGAAATATCGCTTCTTTAGCTATGGCGATGCCATGTTTGTGACGAAAAAAGCCCACTAG

Upstream 100 bases:

>100_bases
GGATCCCGAGCAAGAAGTGGACGCTGGCAATAAGTAATCTTGATATTCTTGGCCTCTAACCATAGACTAGCCGCCGTTCT
AAGCTACCTAGAGGCCTATC

Downstream 100 bases:

>100_bases
ACCAAGATTTTGTTTTAAAATAGCCGCCCAAAGTGAGTATGGGCGGCAACTTTTTTTCGTCAAAAGGTTGAACCTTTTAA
CGCTGTCCCCATCTCTATTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSFILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKDVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH

Sequences:

>Translated_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSFILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKDVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH
>Mature_345_residues
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNNTRVIPARMFGQKASGGKLEI
LVERMLDDKRILAHVRSSKSPKVDSFILLDGGYQMKMVARHDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADK
ERYQTVYNQNPGAVAAPTAGLHFDDAMLDALKAKDVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLPESTLIMLVSAFAGFDHVMAA
YQHAIAQKYRFFSYGDAMFVTKKAH

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=344, Percent_Identity=68.6046511627907, Blast_Score=494, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38156; Mature: 38156

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSFILLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKDVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
CCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAIAQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC
>Mature Secondary Structure
MRVADFSFDLPDELIARYPMAQRNASRLLTLDGNSGALGDKQFTDLLGMINPGDLMVFNN
CEECCCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCEEEECC
TRVIPARMFGQKASGGKLEILVERMLDDKRILAHVRSSKSPKVDSFILLDGGYQMKMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEC
HDTLFELELLSELTILEVLEAVGHMPLPPYIDRPDEDADKERYQTVYNQNPGAVAAPTAG
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCC
LHFDDAMLDALKAKDVNIAFVTLHVGAGTFQPVRVDTILEHKMHSEWANVPQDVVDLIAQ
CCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHH
TKAAGKRVVAVGTTSVRSLESAARASQGELKAFSGDTDIFIYPGYQFQVVDAMVTNFHLP
HHHCCCEEEEEECHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCEEEEHHHHHHHCCCC
ESTLIMLVSAFAGFDHVMAAYQHAIAQKYRFFSYGDAMFVTKKAH
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA