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The map label for this gene is mnmC

Identifier: 126174954

GI number: 126174954

Start: 3225216

End: 3227357

Strand: Direct

Name: mnmC

Synonym: Sbal_2747

Alternate gene names: 126174954

Gene position: 3225216-3227357 (Clockwise)

Preceding gene: 126174944

Following gene: 126174961

Centisome position: 62.9

GC content: 49.58

Gene sequence:

>2142_bases
TTGACTGCAGAGCCCAATAAACCTTGCCAAATTAAGCGTGATTACCAGCAATTAATCAACCTTTATCCCGCAACCGCTGA
CACTGATGCACACTATCTCAGCAAGTTGAGCATTTATCAGCAGCGGGTATTTGAGGCTCATTCACAGCAAAAACTCTTAA
TATTAGGCCAAATTGGTTTAGGTAACGGGCTTGAACTCTTATCTTGGTGGCGCACTCAGGCGAATCCTAGCCAACGTTTA
CTCCTTAAGGTGTTCGAACCCAATCCGATTAATGCGTACGAACTTAAATCACTTTGGGATCAATCACTCGCTCTTGTGAA
AGAAACCCCATTCGAGCCACATCTTGAATCAAAGCAAGCGTCAGCGTTAGAGTGTAAACCTGAGCTCAGCCAACTTGCTC
AAACCTTACTCGATGCTGAGCCGACGGCTATTATAGGTTGCCAGAGATTAATTTTTGATGACGGTCGTACCACTATCGAC
CTGCACTTTGGTGATATTCAAACCCAGCTCAGTTCACTCAGCCATTCGCCAATGCATCCGGTTCAACATTGGTTGATATT
GCCCCATCTGCTACAGGCGCTACATCATCAAAGCCACTGGCAGATGGCAAAACTCAGTGACGATAGCGCGACCATTGCCA
CGATTGGATTAAGCGAATCAAGCGGATTAAGTGAAACAACAGTCAAGCGTTTCCAAGCCTGTGGCTTTACTGTGAGCGAT
ATCAATGAACTTGCTACCAAAGCTCTGGGTATCAATGCTCTTGGTATACATCAGCCAGTCACATTGATTAATCACCAGCC
TGACGCCGTACTGCTACACGAGCGCCAAGTCTTACGTCAGCAAGACGTTAAAGCCTATGCTTTTAATCCTATGGCTGCAA
TCTTATCCTCGGCGACGCCCAGCTCGATTGCCATTATAGGCGGCGGCTTAGCCTCGGCGCATTTAGCCTTGTCTTTAGCC
GAGCGCGGCCAAGGCGCACAGGTATTTTGTAAAGATGCAGAGCTAGGCCAAGGCGCATCGGGCAATCGCCAAGGAGCCAT
TTATCCGCTGCTGACCCCAGAAAATGACGAGCTGAGCCGTTTTTTCCAACAGGCATTTTTATTTAGCCGACGTCGAGTGC
AGGCGCTGACATCCGCCCCCGCTGTTAATCAAACGTCGATTTCCCATGATTTTTGCGGCGTATTGCAAACCGCCCACGAT
GAACGCAGCCAGTTACGACTCGATAAAATCATTCAAGGCCAACCTTGGCCAAGTGAAATCGCCTACGCCGTCGATGCTGA
GCAAGCCAATGCAATTGCCAAGATCAATCTCGATAAGCCAGGCTTTTTCTATCCCTTAGGTGGCTGGGTTTGCCCATTTG
AATATGCCGACGCTGCGATACAAAAAGCGATGCAACTGGCCGATGTGAGCGTCTCGCTCAATACCGACATCCTTGCCATT
GAACGCCAAGCCGATGGCTGGGTTTTGTTGACTGAAAAGGAACGTTTCGGGCCCTTCGCGCAACTTGTACTCGCCAATGG
CGCTGAATTGACACAATTTGATGCCAGTAACAAATTACAAATTAGCCCCTTTCGCGGCCAAGTCAGCCATGTGCCCGCTC
AATTTCAATTGAGTCAGCTCGCAACTGTGCTGTGCGCCAACGGTTATTTAACGCCAAGTCACCAAGGACTCCACTGCCTT
GGCGCGAGTTACGTTAAAGAACCCGAGCATTTAGACTTTTGCCCGCAGGAACAACAAGAAAACCTCATGAAAATGCACGA
GAGTTATCCTGATCACAGCTGGCTTGAAGATATCGACATGAGTGGCAACAATGCCCGCGTCGGCGTGCGTATGGTGACGC
GGGATCATTTCCCTATGATGGGTTGCGCGCCAGACGTGCCTAAAATCCTTAAGGATTATGAGCAACATCAACTCACTAAA
GAAAGTCGCCACTACTGGCAAACCACGCCAGCGCCACTGCATCAAGGACTGTATATTCTTGGCGGGTTAGGCTCACGGGG
ACTCAGTTCTGGGCCACTGGCCGCCGAATGTCTCGCGGCGCAGCTTTGCGGCGAACCAATACCGCTCGATAAAGAAACCC
TGTGTAAACTCAACCCCAATCGCATGTGGCTGCGAAAATTGCTCAAGGGTAAAGCGCTCTAG

Upstream 100 bases:

>100_bases
TTCGCTGAGCCAATAGTATCTGTTTTGACTCAATTAAGTGGTCAGCTTTCCATAAAGGCGAGTAAAATAATGCCAATTAT
GAGACCGAGAGTAAATTTCT

Downstream 100 bases:

>100_bases
GTAATCAATCGCTAGAAAAATAACGTTTTTGAGCGCTATAAAAAAGGGATTAAGCCATGGCTTAATCCCTTTTTTAAATG
TTTTCTGTAAACCACTTAAG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 713; Mature: 712

Protein sequence:

>713_residues
MTAEPNKPCQIKRDYQQLINLYPATADTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRL
LLKVFEPNPINAYELKSLWDQSLALVKETPFEPHLESKQASALECKPELSQLAQTLLDAEPTAIIGCQRLIFDDGRTTID
LHFGDIQTQLSSLSHSPMHPVQHWLILPHLLQALHHQSHWQMAKLSDDSATIATIGLSESSGLSETTVKRFQACGFTVSD
INELATKALGINALGIHQPVTLINHQPDAVLLHERQVLRQQDVKAYAFNPMAAILSSATPSSIAIIGGGLASAHLALSLA
ERGQGAQVFCKDAELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRVQALTSAPAVNQTSISHDFCGVLQTAHD
ERSQLRLDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAI
ERQADGWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCL
GASYVKEPEHLDFCPQEQQENLMKMHESYPDHSWLEDIDMSGNNARVGVRMVTRDHFPMMGCAPDVPKILKDYEQHQLTK
ESRHYWQTTPAPLHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL

Sequences:

>Translated_713_residues
MTAEPNKPCQIKRDYQQLINLYPATADTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRL
LLKVFEPNPINAYELKSLWDQSLALVKETPFEPHLESKQASALECKPELSQLAQTLLDAEPTAIIGCQRLIFDDGRTTID
LHFGDIQTQLSSLSHSPMHPVQHWLILPHLLQALHHQSHWQMAKLSDDSATIATIGLSESSGLSETTVKRFQACGFTVSD
INELATKALGINALGIHQPVTLINHQPDAVLLHERQVLRQQDVKAYAFNPMAAILSSATPSSIAIIGGGLASAHLALSLA
ERGQGAQVFCKDAELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRVQALTSAPAVNQTSISHDFCGVLQTAHD
ERSQLRLDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAI
ERQADGWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCL
GASYVKEPEHLDFCPQEQQENLMKMHESYPDHSWLEDIDMSGNNARVGVRMVTRDHFPMMGCAPDVPKILKDYEQHQLTK
ESRHYWQTTPAPLHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
>Mature_712_residues
TAEPNKPCQIKRDYQQLINLYPATADTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGLGNGLELLSWWRTQANPSQRLL
LKVFEPNPINAYELKSLWDQSLALVKETPFEPHLESKQASALECKPELSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDL
HFGDIQTQLSSLSHSPMHPVQHWLILPHLLQALHHQSHWQMAKLSDDSATIATIGLSESSGLSETTVKRFQACGFTVSDI
NELATKALGINALGIHQPVTLINHQPDAVLLHERQVLRQQDVKAYAFNPMAAILSSATPSSIAIIGGGLASAHLALSLAE
RGQGAQVFCKDAELGQGASGNRQGAIYPLLTPENDELSRFFQQAFLFSRRRVQALTSAPAVNQTSISHDFCGVLQTAHDE
RSQLRLDKIIQGQPWPSEIAYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAIE
RQADGWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQLATVLCANGYLTPSHQGLHCLG
ASYVKEPEHLDFCPQEQQENLMKMHESYPDHSWLEDIDMSGNNARVGVRMVTRDHFPMMGCAPDVPKILKDYEQHQLTKE
SRHYWQTTPAPLHQGLYILGGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=677, Percent_Identity=33.8257016248154, Blast_Score=332, Evalue=7e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_SHEB5 (A3D671)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051103.1
- ProteinModelPortal:   A3D671
- SMR:   A3D671
- STRING:   A3D671
- GeneID:   4845283
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_2747
- NMPDR:   fig|325240.3.peg.1428
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   WLILPHL
- PhylomeDB:   A3D671
- ProtClustDB:   CLSK2516783
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO

EC number: =2.1.1.61

Molecular weight: Translated: 78855; Mature: 78724

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEPNKPCQIKRDYQQLINLYPATADTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGL
CCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GNGLELLSWWRTQANPSQRLLLKVFEPNPINAYELKSLWDQSLALVKETPFEPHLESKQA
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SALECKPELSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDLHFGDIQTQLSSLSHSPMHP
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEEEEECHHHHHHHHCCCCCCHH
VQHWLILPHLLQALHHQSHWQMAKLSDDSATIATIGLSESSGLSETTVKRFQACGFTVSD
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHH
INELATKALGINALGIHQPVTLINHQPDAVLLHERQVLRQQDVKAYAFNPMAAILSSATP
HHHHHHHHHCCCEECCCCCCCEECCCCCEEEEHHHHHHHHHCCHHEECCHHHHHHCCCCC
SSIAIIGGGLASAHLALSLAERGQGAQVFCKDAELGQGASGNRQGAIYPLLTPENDELSR
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHH
FFQQAFLFSRRRVQALTSAPAVNQTSISHDFCGVLQTAHDERSQLRLDKIIQGQPWPSEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAI
EEEEEHHHCCEEEEEECCCCCEEEECCCEECCHHHHHHHHHHHHHHHHEEEEECCCEEEE
ERQADGWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQL
EECCCCEEEEECCHHCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHH
ATVLCANGYLTPSHQGLHCLGASYVKEPEHLDFCPQEQQENLMKMHESYPDHSWLEDIDM
HHHHHCCCCCCCCCCCCHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCC
SGNNARVGVRMVTRDHFPMMGCAPDVPKILKDYEQHQLTKESRHYWQTTPAPLHQGLYIL
CCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEE
GGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
ECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TAEPNKPCQIKRDYQQLINLYPATADTDAHYLSKLSIYQQRVFEAHSQQKLLILGQIGL
CCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
GNGLELLSWWRTQANPSQRLLLKVFEPNPINAYELKSLWDQSLALVKETPFEPHLESKQA
CCCHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
SALECKPELSQLAQTLLDAEPTAIIGCQRLIFDDGRTTIDLHFGDIQTQLSSLSHSPMHP
CCCCCCHHHHHHHHHHHCCCCCHHHHHHHEEEECCCEEEEEEECHHHHHHHHCCCCCCHH
VQHWLILPHLLQALHHQSHWQMAKLSDDSATIATIGLSESSGLSETTVKRFQACGFTVSD
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCHHH
INELATKALGINALGIHQPVTLINHQPDAVLLHERQVLRQQDVKAYAFNPMAAILSSATP
HHHHHHHHHCCCEECCCCCCCEECCCCCEEEEHHHHHHHHHCCHHEECCHHHHHHCCCCC
SSIAIIGGGLASAHLALSLAERGQGAQVFCKDAELGQGASGNRQGAIYPLLTPENDELSR
CEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHHH
FFQQAFLFSRRRVQALTSAPAVNQTSISHDFCGVLQTAHDERSQLRLDKIIQGQPWPSEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
AYAVDAEQANAIAKINLDKPGFFYPLGGWVCPFEYADAAIQKAMQLADVSVSLNTDILAI
EEEEEHHHCCEEEEEECCCCCEEEECCCEECCHHHHHHHHHHHHHHHHEEEEECCCEEEE
ERQADGWVLLTEKERFGPFAQLVLANGAELTQFDASNKLQISPFRGQVSHVPAQFQLSQL
EECCCCEEEEECCHHCCHHHHHHHHCCCCEEEECCCCCEEECCCCCHHHCCCHHHHHHHH
ATVLCANGYLTPSHQGLHCLGASYVKEPEHLDFCPQEQQENLMKMHESYPDHSWLEDIDM
HHHHHCCCCCCCCCCCCHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHCC
SGNNARVGVRMVTRDHFPMMGCAPDVPKILKDYEQHQLTKESRHYWQTTPAPLHQGLYIL
CCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCEEEE
GGLGSRGLSSGPLAAECLAAQLCGEPIPLDKETLCKLNPNRMWLRKLLKGKAL
ECCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA