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The map label for this gene is pdxB [H]

Identifier: 126174952

GI number: 126174952

Start: 3222575

End: 3223705

Strand: Reverse

Name: pdxB [H]

Synonym: Sbal_2745

Alternate gene names: 126174952

Gene position: 3223705-3222575 (Counterclockwise)

Preceding gene: 126174953

Following gene: 126174951

Centisome position: 62.87

GC content: 47.75

Gene sequence:

>1131_bases
ATGAAGATTTTAGTCGATGAAAATATGCCCTATGTTGAACCGCTATTTGGCGATCTCGGGGACATTATTCCTGTTAACGG
CCGCACGTTAACCGCCGAGCAAGTAGGCGAGGCCGATGTGCTATTAGTGCGCTCGGTCACCAAAGTGAATGCCGAGTTGC
TCTCAGGCAATAACAAGCTGAAATTTGTCGGCAGTGCCACCATAGGCACAGATCATGTGGATTTAACCTATCTTGCTGAG
CGTAATATTCCCTTTTCTAATGCACCAGGTTGCAATGCGACTGCGGTCGGGGAGTTTGCTTTTATCGCTATGCTGGAGCT
AGCGCAGCGCTTTAATTCGCCACTTAAGGGCAAGGTCGTCGGCATAGTGGGCGCGGGCAACACTGGCACTGCGACGGCAA
AATGTTTGCAAGCCTATGGTATTAAAGTACTGCTTAACGATCCCATCAAAGCTGCAGAGGGCGATCCGCGCAGTTTTGTG
AGCTTAGAAACCATCATGGCGCAGGCCGATATCATCAGTTTACATGTGCCCATCACGCGCACTGGCGAGCATAAAACCAA
GTATTTGTTCGATGAGGCGCGCTTAAAGGCACTCAAACCCAATACATGGTTAGTGAATTGCTGCCGCGGTGATGTGATAG
ATAATCAGGCGCTTATTAAAGTGAAGCAGCAACGTGACGATCTTAAATTAGTGCTCGATGTGTGGGAAGGCGAGCCAACA
CCTCTGCCCGAGTTGGTACCATTGGCAGAGTTTGCTACGCCGCATATCGCAGGTTATAGCTTAGAAGGTAAGGCGCGCGG
CACTTTTATGCTCTACCAAAAATTGTGTCAGTTGTTGAATATTACTGCCGATAAAAGCCTGCTGGATCTCTTGCCAACCT
TTAACATTAAGGCGGTCGAACTTGCGACCGCGCCGAATGAAAAAGCGTTATTGCAGCTCGCACGTTTTGTCTATGATTTG
CGCGATGATGACAAGATGTTCAGAAATACATTCTTAAATGAGAATGGCTTCGACACTATGAGAAAAAATCACCAACATAG
ACGCGAATTTAGTGCATTGGCGCTGGCTTATGACGGTCAATCTGAGGTAGATTGGTTGTCCAATTTAGGTTTTTCAGGAG
TCGGTCAGTAG

Upstream 100 bases:

>100_bases
TGTAAACCTGTTGCATCGACAAGGATTTATCCCATTAGTCTTGTTAGAATACCGAGCATCTTGATGCCTTCCATTCCCAC
TTTTTTTATATCAGGTGCAC

Downstream 100 bases:

>100_bases
CTATGTCACAGGAATTTAATGTTGTCGTTTTAGGTGCATCGGGCGCAGTGGGTCAAACAATGATAGAGATCCTCGAAGAG
CGTAATTTCCCGGTTGCCAA

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKILVDENMPYVEPLFGDLGDIIPVNGRTLTAEQVGEADVLLVRSVTKVNAELLSGNNKLKFVGSATIGTDHVDLTYLAE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKAAEGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKYLFDEARLKALKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPT
PLPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNITADKSLLDLLPTFNIKAVELATAPNEKALLQLARFVYDL
RDDDKMFRNTFLNENGFDTMRKNHQHRREFSALALAYDGQSEVDWLSNLGFSGVGQ

Sequences:

>Translated_376_residues
MKILVDENMPYVEPLFGDLGDIIPVNGRTLTAEQVGEADVLLVRSVTKVNAELLSGNNKLKFVGSATIGTDHVDLTYLAE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKAAEGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKYLFDEARLKALKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPT
PLPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNITADKSLLDLLPTFNIKAVELATAPNEKALLQLARFVYDL
RDDDKMFRNTFLNENGFDTMRKNHQHRREFSALALAYDGQSEVDWLSNLGFSGVGQ
>Mature_376_residues
MKILVDENMPYVEPLFGDLGDIIPVNGRTLTAEQVGEADVLLVRSVTKVNAELLSGNNKLKFVGSATIGTDHVDLTYLAE
RNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVVGIVGAGNTGTATAKCLQAYGIKVLLNDPIKAAEGDPRSFV
SLETIMAQADIISLHVPITRTGEHKTKYLFDEARLKALKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPT
PLPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNITADKSLLDLLPTFNIKAVELATAPNEKALLQLARFVYDL
RDDDKMFRNTFLNENGFDTMRKNHQHRREFSALALAYDGQSEVDWLSNLGFSGVGQ

Specific function: Catalyzes the oxidation of erythronate-4-phosphate to 3- hydroxy-2-oxo-4-phosphonooxybutanoate [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. PdxB subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=346, Percent_Identity=26.5895953757225, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI145580578, Length=226, Percent_Identity=25.6637168141593, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4557499, Length=226, Percent_Identity=25.6637168141593, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI145580575, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4557497, Length=254, Percent_Identity=23.6220472440945, Blast_Score=65, Evalue=9e-11,
Organism=Homo sapiens, GI61743967, Length=254, Percent_Identity=23.6220472440945, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1788660, Length=374, Percent_Identity=44.9197860962567, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1789279, Length=259, Percent_Identity=28.957528957529, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787645, Length=199, Percent_Identity=28.1407035175879, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=26, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=252, Percent_Identity=29.3650793650794, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322116, Length=318, Percent_Identity=25.4716981132075, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=318, Percent_Identity=25.4716981132075, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=225, Percent_Identity=24.4444444444444, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921140, Length=260, Percent_Identity=28.0769230769231, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24646446, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24646448, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24646452, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24646450, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI62472511, Length=239, Percent_Identity=26.7782426778243, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574284, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585514, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574282, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551003, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45552429, Length=272, Percent_Identity=24.6323529411765, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=25.187969924812, Blast_Score=68, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR020921
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.290 [H]

Molecular weight: Translated: 41349; Mature: 41349

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVDENMPYVEPLFGDLGDIIPVNGRTLTAEQVGEADVLLVRSVTKVNAELLSGNNKL
CEEEECCCCCCHHHHHHCCCCEEECCCCEEEHHHCCCCCEEEEHHHHHHHHHHHCCCCEE
KFVGSATIGTDHVDLTYLAERNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVV
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTATAKCLQAYGIKVLLNDPIKAAEGDPRSFVSLETIMAQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECEEE
TGEHKTKYLFDEARLKALKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPT
CCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHEEECCCCCCEEEEEEEECCCCC
PLPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNITADKSLLDLLPTFNIKAVE
CCCHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEE
LATAPNEKALLQLARFVYDLRDDDKMFRNTFLNENGFDTMRKNHQHRREFSALALAYDGQ
EECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEECCC
SEVDWLSNLGFSGVGQ
CHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKILVDENMPYVEPLFGDLGDIIPVNGRTLTAEQVGEADVLLVRSVTKVNAELLSGNNKL
CEEEECCCCCCHHHHHHCCCCEEECCCCEEEHHHCCCCCEEEEHHHHHHHHHHHCCCCEE
KFVGSATIGTDHVDLTYLAERNIPFSNAPGCNATAVGEFAFIAMLELAQRFNSPLKGKVV
EEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEE
GIVGAGNTGTATAKCLQAYGIKVLLNDPIKAAEGDPRSFVSLETIMAQADIISLHVPITR
EEEECCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECEEE
TGEHKTKYLFDEARLKALKPNTWLVNCCRGDVIDNQALIKVKQQRDDLKLVLDVWEGEPT
CCCCHHHHHHHHHHHHEECCCCEEEEECCCCCCCCHHHEEECCCCCCEEEEEEEECCCCC
PLPELVPLAEFATPHIAGYSLEGKARGTFMLYQKLCQLLNITADKSLLDLLPTFNIKAVE
CCCHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEE
LATAPNEKALLQLARFVYDLRDDDKMFRNTFLNENGFDTMRKNHQHRREFSALALAYDGQ
EECCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEEEECCC
SEVDWLSNLGFSGVGQ
CHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA