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The map label for this gene is ackA [H]

Identifier: 126174883

GI number: 126174883

Start: 3136366

End: 3137565

Strand: Direct

Name: ackA [H]

Synonym: Sbal_2674

Alternate gene names: 126174883

Gene position: 3136366-3137565 (Clockwise)

Preceding gene: 126174881

Following gene: 126174884

Centisome position: 61.17

GC content: 47.17

Gene sequence:

>1200_bases
ATGTCAAATAATCTGGTTTTAGTTCTCAATTGCGGTAGCTCTTCTCTCAAATTTGCCGTCATTGACGCGCAAACAGGTGA
CGATCAAATTTCTGGCTTAGCCGAATGTTTTGGTTTAGAAGACTCGCGCATTAAATGGAAAATCAACGGCGAAAAACATG
AAGCGGCCTTAGGCGCATTTACGGCTCACCGTGAAGCAGTTGAATACATTGTTAATAAAATCCTTGCGGAACAGCCTGAA
TTAGCAGCGAAAATTCAAGCCGTTGGCCACCGTATCGTTCACGGCGGAGAAAAGTTCACTCGTTCTGTGATCATCGACGA
AAGCGTGATCAAAGGTATCGAAGATTGTGCTTCATTAGCCCCCCTGCACAATCCTGCTCACCTTATCGGCATCCGTGCAG
CCATTGCCTCTTTCCCTAAATTGCCACAGGTTGCCGTATTCGATACCGCATTCCACCAAAGCATGCCTGACAGAGCCTAT
GTCTACGCCCTGCCTTACAAGTTATACCGTGAACACGGTATCCGTCGTTATGGCATGCATGGCACGAGCCATTTATTCGT
TAGCCGTGAAGCGGCAAAAATGCTTAACAAGCCCATTGAAGAAACTAACGTTATCTGTGCTCACTTGGGCAACGGCGCGT
CAGTGACTGCCATCAAAGGCGGCAAAAGTGTCGACACTTCTATGGGCTTAACCCCATTAGAAGGTTTGGTCATGGGCACA
CGTTGTGGCGATATTGACCCTTCAATCATCTATCACTTAGTTCACCAACTGGGTTACACCTTAGAAGAAGTGAATAACCT
GATGAACAAGCAAAGTGGTCTGCTGGGGATTTCCGAGCTGACTAACGATTGCCGCGGTATCGAAGAAGGTTACGCCGACG
GCCATAAAGGCGCGACCTTAGCCTTAGAAATTTTCTGCTACCGTTTAGCCAAATACATCGCCTCTTACACTGTGCCACTG
GGCCGTTTAGATGCAGTCGTCTTCACCGGCGGCATTGGCGAAAACTCAGACTTAATCCGCGAAAAAGTGCTCAACATGCT
GGAAATCTTCAATTTCCATGTCGATAGCGAACGCAACAAAGCGGCGCGTTTCGGTAAGAAAGGCATCATCACTCAAGATA
AAGGCACGATTGCCATGGTGATCCCAACGAACGAAGAATGGGTAATCGCCGAAGATTCAATTAAACTGATCAACAAATAA

Upstream 100 bases:

>100_bases
CACAATCTGGCGAATCGCCCCAAGACTGTTATGGTAAAAACACCGCAGATCCTAATTTTTTTGAATCGTTATATTAAACA
CAATGGCAAAGGGTTTACAC

Downstream 100 bases:

>100_bases
AAATGACCTAAGCCGTTTGCTCACCCAAACGGCTTTACGCTTTACATAGCCATACTCACAAGCAAATTTGCAGAAACTCT
TCTGCCAAAAACCATCGAAA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPE
LAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAY
VYALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK

Sequences:

>Translated_399_residues
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPE
LAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAY
VYALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPL
GRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
>Mature_398_residues
SNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAFTAHREAVEYIVNKILAEQPEL
AAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLAPLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYV
YALPYKLYREHGIRRYGMHGTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGTR
CGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATLALEIFCYRLAKYIASYTVPLG
RLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNKAARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=64.6616541353383, Blast_Score=540, Evalue=1e-155,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=45.5470737913486, Blast_Score=342, Evalue=3e-95,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43601; Mature: 43469

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAF
CCCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TAHREAVEYIVNKILAEQPELAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYVYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHCCCCHHHCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
HHHCCCCCCEEECCCCEEEEEECCCCEEEECCCHHHCCC
>Mature Secondary Structure 
SNNLVLVLNCGSSSLKFAVIDAQTGDDQISGLAECFGLEDSRIKWKINGEKHEAALGAF
CCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
TAHREAVEYIVNKILAEQPELAAKIQAVGHRIVHGGEKFTRSVIIDESVIKGIEDCASLA
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAIASFPKLPQVAVFDTAFHQSMPDRAYVYALPYKLYREHGIRRYGMH
CCCCCHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCCCEEEEECCHHHHHHCCCEEECCC
GTSHLFVSREAAKMLNKPIEETNVICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCCEEEEEHHHHHHHCCCCHHHCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDIDPSIIYHLVHQLGYTLEEVNNLMNKQSGLLGISELTNDCRGIEEGYADGHKGATL
CCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCCHH
ALEIFCYRLAKYIASYTVPLGRLDAVVFTGGIGENSDLIREKVLNMLEIFNFHVDSERNK
HHHHHHHHHHHHHHHHCCCCHHHCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHH
AARFGKKGIITQDKGTIAMVIPTNEEWVIAEDSIKLINK
HHHCCCCCCEEECCCCEEEEEECCCCEEEECCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA