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The map label for this gene is focA [H]

Identifier: 126174879

GI number: 126174879

Start: 3130387

End: 3131379

Strand: Reverse

Name: focA [H]

Synonym: Sbal_2670

Alternate gene names: 126174879

Gene position: 3131379-3130387 (Counterclockwise)

Preceding gene: 126174882

Following gene: 126174877

Centisome position: 61.07

GC content: 46.93

Gene sequence:

>993_bases
ATGAAAAGCCCAAATCCATCATCAACCCCAACGGTGACAAACACTGCCCACGAAGCACCACAGGTTTCAGCGCACTTTAG
TTGTTACAATCAGGCTGAAATTTACGGTACCAGCAAAGTGGTTAAAGCGCCTTGGCAATCCTTTGGCCTGGCGGTGTTTG
CTGGCGCCTTCATCGCCTTAGCCTTTGTATTTTATCTGACAGTGACCACAGGTGCTGGCGATGCGTCTTGGGGTTTAGTT
CGCTTCGTTGGTGGGATAGCCTTTAGTTTAGGCCTGATTTTAGTGGTTGTTTGCGGCGGCGAGCTGTTCACCAGTTCAGT
ACTCAGTACCGTCGCCTGGGCACAAAAACAAGTCTCTGGCGCTGAATTACTCAAATGTTGGTGCCGGGTTTATGCGGGCA
ATTTTGTCGGTGCTATACTGATGGTTTGCTTAATCACAGCCGCGGGCATGTATGAACTCGATGGCGGTAACTGGGGATTA
AATGCCCTTAAAGTTGCGCAACACAAACTGCATCACACTTGGATCCAAGCCTTTAGCTTAGGCATCTTATGTAACATGTT
GGTGTGTTTAGGCGTGTGGATGACCTTTGCCAGTCGCGATGTGTTGACTAAATCCCTACTGTTGATCTTACCCGTGGCCA
TGTTCGTCAGCAGCGGTTTTGAACACAGTATCGCTAACCTGTTTATGGTGCCTATGGGCATGATGATCCAATCTATCGCC
AGCCCAGAATTCTTTGCATCTTTAGGTGTGACTCAAGAGCAGTTTGCCGATTTAACTGTAACTAACTTTATATTCAACAA
CTTAATTCCAGTCACTTTAGGTAATATTGTCGGCGGCGGTGTGATTGTGGGTTTAGGTTATTGGTGGATAGAGCAGGGGA
AAACGATGTCAAGCGATCCAAACAAAGCTCACCATGAGCACTTTTCTGCATCCCATTCACATAACGAAAAACCTCAACAT
GCGAGTGAAATTAAGCTGACGAAGCATTCCTAG

Upstream 100 bases:

>100_bases
TCAACATCTCGGTTTTGATTCGTGCTATCTGCGCAACGGCGCTGCAGGAGAGATATATGCTACGCATTCAAGTCTGTTTA
TCTGAAGTTGGAGTCATGCA

Downstream 100 bases:

>100_bases
AATGTTTTAAATGACCCGTTGAAGGATAAGAAAATGCCCAGCAAATAGTTGGGCATTTTTTATGCTTATTTACTGGGCTG
CGTAAAAGAAGCAAATAAAA

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKSPNPSSTPTVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTMSSDPNKAHHEHFSASHSHNEKPQH
ASEIKLTKHS

Sequences:

>Translated_330_residues
MKSPNPSSTPTVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTMSSDPNKAHHEHFSASHSHNEKPQH
ASEIKLTKHS
>Mature_330_residues
MKSPNPSSTPTVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDASWGLV
RFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSGAELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGL
NALKVAQHKLHHTWIQAFSLGILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTMSSDPNKAHHEHFSASHSHNEKPQH
ASEIKLTKHS

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=259, Percent_Identity=55.5984555984556, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1788837, Length=256, Percent_Identity=40.625, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082249, Length=247, Percent_Identity=28.3400809716599, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321779, Length=206, Percent_Identity=28.1553398058252, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 35553; Mature: 35553

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00589 PTS_HPR_SER ; PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSPNPSSTPTVTNTAHEAPQVSAHFSCYNQAEIYGTSKVVKAPWQSFGLAVFAGAFIAL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHCCCHHHHHCCHHHHHHHHHHHHHHHH
AFVFYLTVTTGAGDASWGLVRFVGGIAFSLGLILVVVCGGELFTSSVLSTVAWAQKQVSG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCH
AELLKCWCRVYAGNFVGAILMVCLITAAGMYELDGGNWGLNALKVAQHKLHHTWIQAFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GILCNMLVCLGVWMTFASRDVLTKSLLLILPVAMFVSSGFEHSIANLFMVPMGMMIQSIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
SPEFFASLGVTQEQFADLTVTNFIFNNLIPVTLGNIVGGGVIVGLGYWWIEQGKTMSSDP
CHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCC
NKAHHEHFSASHSHNEKPQHASEIKLTKHS
CHHHHHHHCCCCCCCCCCCCHHHCEEECCC
>Mature Secondary Structure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CHHHHHHHCCCCCCCCCCCCHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]