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The map label for this gene is cysK [H]

Identifier: 126174873

GI number: 126174873

Start: 3121126

End: 3122094

Strand: Direct

Name: cysK [H]

Synonym: Sbal_2663

Alternate gene names: 126174873

Gene position: 3121126-3122094 (Clockwise)

Preceding gene: 126174871

Following gene: 126174874

Centisome position: 60.87

GC content: 47.16

Gene sequence:

>969_bases
ATGAGCAAAATTTTCGAAGACAATTCATATACTATTGGTAATACCCCGCTAGCACGTTTAAACCGTGTTAGCCAAGGTAA
AGTGCTGGCCAAAGTCGAAGCGCGGAACCCAAGTTTCAGTGTTAAATGCCGTATCGGTGCCAATATGATTTGGGATGCCG
AGAAAAAGGGTCTACTGACTAAAGACCACGAGCTGATTGAGCCGACGTCTGGCAACACAGGTATTGCGCTGGCGTATGTA
GCCGCGGCCCGTGGTTATAAGCTTACCCTGACGATGCCAAATACCATGAGTTTAGAGCGTCGTAAGCTGCTCAAAGCCCT
TGGCGCAAATCTCGTGTTGACAGAAGGCGCGAAAGGCATGAAAGGTGCGATTGATAAAGCGGAAGAAATCCGTCAATCGG
CTCCTGAAAAATACATTTTATTACAGCAGTTTAATAACCCTGCTAACCCAGAAATCCATGAAAAAACCACAGGTCCAGAA
ATCTGGAATGATACCGATGGCGAAGTGGATGTGTTTGTTGCCGGTGTAGGTACGGGCGGTACGATTACAGGTGTAAGCCG
TTACTTGAAAAAAGTAGCAGGCAAAACCATTACCTCAGTGGCGGTTGAGCCAGCAGATTCTCCAGTGATTGCGCAAACCT
TAGCGGGTTTACCGGTACAACCTGGACCACATAAAATCCAAGGTATTGGCGCAGGCTTTATCCCTGGTAACTTAGATTTA
GAGTTGATTGACCGCGTAGAAGCTGTCACCAATGATGAAGCCATCGACATGGCCCGTCGCTTAATGCAAGAAGAAGGCAT
TTTAGTGGGTATTTCATCGGGTGCTGCCGTTGTTGCCGCAAACCGCATCGCTGAATTGCCAGAATTTGCGGGTAAAACCA
TTGTGGTAATTTTGCCATCGGCTGCCGAACGTTACTTATCTTCGGTGCTATTCCAAGGCCAATTCGGCGACGAAGAAAAC
ATTCAATAA

Upstream 100 bases:

>100_bases
CCTTTAATAACCAAATCGAATAAAGAAGAACAAACAATCACTTCTAAAAGCGCCACAGCAGATTATGATTCAGTAGTGCC
AACATAGGAGCAAGCAATCC

Downstream 100 bases:

>100_bases
TCGATGTTGGATTACCCTTTAAAATACCCAGCCTCTGCTGGGTATTTTTATTTGTGACATTGCATTAATCAGATCCCAGC
TGCAGAGCGCAAACAAGTTC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIFEDNSYTIGNTPLARLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYILLQQFNNPANPEIHEKTTGPE
IWNDTDGEVDVFVAGVGTGGTITGVSRYLKKVAGKTITSVAVEPADSPVIAQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVEAVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAELPEFAGKTIVVILPSAAERYLSSVLFQGQFGDEEN
IQ

Sequences:

>Translated_322_residues
MSKIFEDNSYTIGNTPLARLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYILLQQFNNPANPEIHEKTTGPE
IWNDTDGEVDVFVAGVGTGGTITGVSRYLKKVAGKTITSVAVEPADSPVIAQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVEAVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAELPEFAGKTIVVILPSAAERYLSSVLFQGQFGDEEN
IQ
>Mature_321_residues
SKIFEDNSYTIGNTPLARLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLTKDHELIEPTSGNTGIALAYVA
AARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIDKAEEIRQSAPEKYILLQQFNNPANPEIHEKTTGPEI
WNDTDGEVDVFVAGVGTGGTITGVSRYLKKVAGKTITSVAVEPADSPVIAQTLAGLPVQPGPHKIQGIGAGFIPGNLDLE
LIDRVEAVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAELPEFAGKTIVVILPSAAERYLSSVLFQGQFGDEENI
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=334, Percent_Identity=38.9221556886228, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI295821200, Length=334, Percent_Identity=38.9221556886228, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI4557415, Length=334, Percent_Identity=38.9221556886228, Blast_Score=193, Evalue=2e-49,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=76.875, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI2367138, Length=315, Percent_Identity=42.2222222222222, Blast_Score=210, Evalue=8e-56,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=53.9473684210526, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17562970, Length=313, Percent_Identity=49.2012779552716, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=53.8216560509554, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=50.1628664495114, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI17561720, Length=313, Percent_Identity=48.2428115015974, Blast_Score=244, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=50.4545454545455, Blast_Score=204, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=39.4321766561514, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=36.4779874213836, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17561716, Length=125, Percent_Identity=50.4, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=325, Percent_Identity=37.2307692307692, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=334, Percent_Identity=32.9341317365269, Blast_Score=132, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24643623, Length=307, Percent_Identity=37.1335504885993, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI20129101, Length=307, Percent_Identity=37.1335504885993, Blast_Score=154, Evalue=7e-38,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34405; Mature: 34274

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFEDNSYTIGNTPLARLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLT
CCCEECCCCEEECCCCHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDHELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCCHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQSAPEKYILLQQFNNPANPEIHEKTTGPEIWNDTDGEVDVFVAGVGTGG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYLKKVAGKTITSVAVEPADSPVIAQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVEAVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAELPEFAGKTIVVILPS
HHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECC
AAERYLSSVLFQGQFGDEENIQ
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKIFEDNSYTIGNTPLARLNRVSQGKVLAKVEARNPSFSVKCRIGANMIWDAEKKGLLT
CCEECCCCEEECCCCHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDHELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCCHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCC
KGAIDKAEEIRQSAPEKYILLQQFNNPANPEIHEKTTGPEIWNDTDGEVDVFVAGVGTGG
HHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYLKKVAGKTITSVAVEPADSPVIAQTLAGLPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVEAVTNDEAIDMARRLMQEEGILVGISSGAAVVAANRIAELPEFAGKTIVVILPS
HHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHCCCEEEEEECC
AAERYLSSVLFQGQFGDEENIQ
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]