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The map label for this gene is rlmL

Identifier: 126174783

GI number: 126174783

Start: 3012941

End: 3015070

Strand: Reverse

Name: rlmL

Synonym: Sbal_2570

Alternate gene names: 126174783

Gene position: 3015070-3012941 (Counterclockwise)

Preceding gene: 126174784

Following gene: 126174782

Centisome position: 58.8

GC content: 45.87

Gene sequence:

>2130_bases
ATGCTTAATTTTTTTGCCGCCGCCCCTAAGGGCTTTGAATATAGCTTAGCCCAAGAGCTGACAGAATTTGGTGCGACTGA
AGTAAAAGAGAGTGTTGCTGGTGTGTATTTCACGGCCTCCTTAACTTTAGCGTATCGCATTACTTTATGGACACGCTTAG
CCAGCCGCATTGTGTTGGTTATCTATAAAGGAAGCTGCGAGTCGGCAGAACAACTCTATAATGCAGCTTATTGTGTTGAT
TGGCCGGCACACTTTTCCAATAAAAGCACTTTTAGTATCGATTTCCATGGTACGGGTGGTTTTCTTAATAATACCCAGTT
TGGCGCCTTGAAGATTAAGGATGCGATTGTCGATCGTTTCCGCGACGATGATATTGAGCGTCCGAATGTGTCTCGCGTTG
ATGCTGAATTTAAAGTCGATGCGCATTTCCGTAATGGCGTGATCACGATTGCGATGAACTTCTCGGGTCCATCGCTTCAT
CAACGTGGCTATCGTTCGACCACAGGTGAAGCGCCATTGAAGGAAAACTTAGCCGCCAACATGCTCGTACGCAGTGGCTG
GCAAGCGTCGCCTTCAACCTTGCTCGATCCTTTCTGTGGTAGCGGTACAGTGCTGATTGAAGCGGCGTTAATGGCGGCTG
ATATTGCCCCTGGTCTGCAACGTAGTCGTTTTGGTTTTGAACATTGGCGTCGCCATGATAAAGCCGTGTGGCAAGAAATC
GTTGAAGAAGCGAAAGCGCGTGCTTCATTAGGCGTGAAACGTTGCGAGATTAAATTCTACGGTTCTGATATCGATTCGCG
CTTAGTCGCGTTAGCGAAACGTAACGCCGAAAATGCCGGTGTACTGGAGTTAATCGAGTTTAACGTTGCGGATGCTTTAA
CGATAGCGCCGCCTGCGGAAAGCGGCTATTTGATCACCAACCCACCTTATGGTGAGCGTTTAGGCAATGTGTCTGAGTTA
TTGCAGCTTTACTATCAGCTGGGCGACAAGTTTAAGAAAGAGTTTGGCGGCTGGAAAGTGGCCATGCTGTGTAGTGATAT
CGAACTGGTGTCATCGCTAAAGCTTAAAGCCGATAAACAAATGAAGATGTTTAACGGCGCACTTGAATGTGCTTTTAACA
TCTACACCTTACATGCCAACAGTACTCGCCGTGATACGCCAGTGCTGCCAGATGGCGTCGACATTGCTGATATTGCCCCA
GCTTTTGCTAACCGCATTAAGAAGAATGCCAAGTTATTAGAGAAATGGGCGAAGAAAGAAGGCATCGACAGCTATCGTAT
TTACGATGCCGATATTCCTGAATATAACGTCGCAGTAGATAAGTATTTAGATTACGTGATTATTCAAGAGTATATGGCGC
CAGCGACCATACCTGAAGCCGTGACTAAACGTCGTTTAAGTGATGTGTTATTAGCACTGCCATCGGCCATTGGCATCAAT
CCTAACAAGATGATCATGAAGACGCGTGAGCGTCAAAAGGGCACGAGTCAATATCAAAAGCTGGATGAGCGCAAGCTAGA
ACTTATTACCACTGAATATGGCGCTAAGTTCAAGTTGAACTTAACCGGTTATTTGGATACAGGTTTATTCCTCGATCACA
GATTGACACGTCGTTTAGTCGGTCAAAAATCTAAAGGTCGCCGCGTACTGAACTTGTTCTCTTACACAGGTTCTGCTTCT
GTGCATGCAGCGCTTGGCGGTGCTAAGTCGGTGACGACGGTCGATATGTCCAATACCTATATCGCTTGGGCGAAGGACAA
CTTTGCGCTCAATGGCCTGCAAGGTAAGCAGTATGAGTTTGTTCAATCTGACTGCATGCAGTGGATTAGGGATTGTAATG
AACAGTACGATCTGATTTTTATCGATCCGCCGACCTTCTCAAACTCTAAGCGCATGGAAGATTCGTTTGATGTGCAACGG
GATCATGTGAATTTACTGTCATCCTTAGTGAAACTGCTCAGCCCAACGGGCGAGCTAGTGTTCTCTAACAATAAACGTAA
GTTTAAGATGGACATAGAGACGCTGACAAAGATGAACATTAATGTGACAAACATTGATGACGTGACTTTACCTATGGACT
ACAAACGTAATCCACATATCCATAACACCTGGCTTATCACCCATGCCTGA

Upstream 100 bases:

>100_bases
AGACAGTGACTAAATGGGGCTTTGGCTAATAACTGTTTATCTTTTATGTATCCGAGCAGGTATAATGTCGCTTTGATTTT
GACTGCGTACTGATTATCCC

Downstream 100 bases:

>100_bases
GTTGACTCAAGCAGAGGGGGATAACAAGTCTTATCTGCTATATCACACCGATGGTTGCCATCTGTGTGAACTGGCCGCGG
CGTTACTGGACGCGGCCGAC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLTLAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFNVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLSSLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA

Sequences:

>Translated_709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLTLAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFNVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLSSLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
>Mature_709_residues
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLTLAYRITLWTRLASRIVLVIYKGSCESAEQLYNAAYCVD
WPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRFRDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLH
QRGYRSTTGEAPLKENLAANMLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFNVADALTIAPPAESGYLITNPPYGERLGNVSEL
LQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQMKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAP
AFANRIKKNAKLLEKWAKKEGIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLVGQKSKGRRVLNLFSYTGSAS
VHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEFVQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQR
DHVNLLSSLVKLLSPTGELVFSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=713, Percent_Identity=49.0883590462833, Blast_Score=702, Evalue=0.0,
Organism=Escherichia coli, GI145693111, Length=246, Percent_Identity=28.0487804878049, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_SHEB5 (A3D5Q0)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001050932.1
- ProteinModelPortal:   A3D5Q0
- SMR:   A3D5Q0
- STRING:   A3D5Q0
- GeneID:   4843642
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_2570
- NMPDR:   fig|325240.3.peg.3896
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   A3D5Q0
- ProtClustDB:   PRK11783
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 79713; Mature: 79713

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLTLAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHCCEEEE
IYKGSCESAEQLYNAAYCVDWPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
HHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFNVADALTIAPPAE
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCC
SGYLITNPPYGERLGNVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQ
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAPAFANRIKKNAKLLEKWAKKE
HHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCEEEECCCCCCHHH
VQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLSSLVKLLSPTGELV
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
FSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
ECCCCEEEEEEHHHHHEECCEEECCCCEEECCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MLNFFAAAPKGFEYSLAQELTEFGATEVKESVAGVYFTASLTLAYRITLWTRLASRIVLV
CCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHCCEEEE
IYKGSCESAEQLYNAAYCVDWPAHFSNKSTFSIDFHGTGGFLNNTQFGALKIKDAIVDRF
EECCCCHHHHHHHCCEEEEECCHHCCCCCEEEEEECCCCCCCCCCCCCEEEHHHHHHHHH
RDDDIERPNVSRVDAEFKVDAHFRNGVITIAMNFSGPSLHQRGYRSTTGEAPLKENLAAN
CCCCCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHH
MLVRSGWQASPSTLLDPFCGSGTVLIEAALMAADIAPGLQRSRFGFEHWRRHDKAVWQEI
HHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHH
VEEAKARASLGVKRCEIKFYGSDIDSRLVALAKRNAENAGVLELIEFNVADALTIAPPAE
HHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCC
SGYLITNPPYGERLGNVSELLQLYYQLGDKFKKEFGGWKVAMLCSDIELVSSLKLKADKQ
CCEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCHH
MKMFNGALECAFNIYTLHANSTRRDTPVLPDGVDIADIAPAFANRIKKNAKLLEKWAKKE
HHHHHHHEEEEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIDSYRIYDADIPEYNVAVDKYLDYVIIQEYMAPATIPEAVTKRRLSDVLLALPSAIGIN
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PNKMIMKTRERQKGTSQYQKLDERKLELITTEYGAKFKLNLTGYLDTGLFLDHRLTRRLV
CCHHHHHHHHHHCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEECCCHHHHHHHHHHHH
GQKSKGRRVLNLFSYTGSASVHAALGGAKSVTTVDMSNTYIAWAKDNFALNGLQGKQYEF
CCCCCCCEEEEEEECCCCCCEEEECCCCCCEEEEECCCCEEEEECCCEEEECCCCCCHHH
VQSDCMQWIRDCNEQYDLIFIDPPTFSNSKRMEDSFDVQRDHVNLLSSLVKLLSPTGELV
HHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEE
FSNNKRKFKMDIETLTKMNINVTNIDDVTLPMDYKRNPHIHNTWLITHA
ECCCCEEEEEEHHHHHEECCEEECCCCEEECCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA