Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 126174642

GI number: 126174642

Start: 2830920

End: 2831741

Strand: Direct

Name: hisH [H]

Synonym: Sbal_2429

Alternate gene names: 126174642

Gene position: 2830920-2831741 (Clockwise)

Preceding gene: 126174641

Following gene: 126174643

Centisome position: 55.21

GC content: 50.12

Gene sequence:

>822_bases
ATGCAACAAGACACTCAACAATCTAAACTAGCTCAGTTAAGCGCTAATGAGCCACAAGATACTGCACTGGCAGCTAACAG
TGCAGATGCTGTGACAAATGACGCCGCAGAAACCGTCATCATAGATACGGGCTGCGCCAATTTAAGCTCAGTACGTTTCG
CCTTCGAGCGTTTAGGCGCTAAGGTGTTGGTCACTGACGATAAAGCCAAAATCAAAGCGGCAAAACGTGTGGTGCTGCCT
GGTGTGGGCACAGCGGGTGCCGCCATGGCGTCCTTACATGAAAAAGGTTTAGTCGAACTCATCCAAGGCCTGACTCAACC
TGTGCTGGGCGTATGTCTTGGCATGCAAATGTTGGCGGCAATTTCTAAGGAGCGCGGCGGAAAAGGTCAAGATTGCGAGT
GCTTAGGCATAATCCCAACCGACATTTGTGAACTGGATACGCAGTTACTCAAAGCTGAAAGCTTACCTTTGCCACACATG
GGTTGGAACCAACTTAACGTCTCCAACAGCCCTAACGTTCACCCGCTGTTTGCGGGTATTGAAGATGGCAGTTATGTGTA
TTTCGTCCACAGTTATCGCGCGCCGTTGAGCGAATTCACCTTAGCCGAATGTACTTATGGTGAAGGTTTTAGCGCCGCCA
TTGGCAAAGGCAACTTTATGGGCGTGCAGTTCCATCCCGAAAAAAGTGCCGCCGTGGGCGCGCAAATGCTGCGTAACTTT
TTACAGATGGACGAGTTACAGATGAACAAAGCTCTGATGAACCAAGTGCTGCAAAGCAGCTCTGCTGATGGCCAAGCGGG
TGCAAAGAAGGCTGACCAATGA

Upstream 100 bases:

>100_bases
GGCCGCGCGCTACGCCAAGCGGTTAAAGTCGAAGGCGATATTCTGCCATCGAGTAAAGGCGTACTTTAAGGCATCAGCAT
AAATCGTTTAAGAGGTTAGG

Downstream 100 bases:

>100_bases
ATAAATTACTTGTTAAAAAACCAGTTACTAATAAATTACTTATAAACAAATCAATAAAGGATGTGAGCTTATGATTATTC
CTGCGATTGATTTAATTGAT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MQQDTQQSKLAQLSANEPQDTALAANSADAVTNDAAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLP
GVGTAGAAMASLHEKGLVELIQGLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHM
GWNQLNVSNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQMLRNF
LQMDELQMNKALMNQVLQSSSADGQAGAKKADQ

Sequences:

>Translated_273_residues
MQQDTQQSKLAQLSANEPQDTALAANSADAVTNDAAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLP
GVGTAGAAMASLHEKGLVELIQGLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHM
GWNQLNVSNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQMLRNF
LQMDELQMNKALMNQVLQSSSADGQAGAKKADQ
>Mature_273_residues
MQQDTQQSKLAQLSANEPQDTALAANSADAVTNDAAETVIIDTGCANLSSVRFAFERLGAKVLVTDDKAKIKAAKRVVLP
GVGTAGAAMASLHEKGLVELIQGLTQPVLGVCLGMQMLAAISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHM
GWNQLNVSNSPNVHPLFAGIEDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQMLRNF
LQMDELQMNKALMNQVLQSSSADGQAGAKKADQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=49.7630331753554, Blast_Score=202, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=32.8767123287671, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 28944; Mature: 28944

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDTQQSKLAQLSANEPQDTALAANSADAVTNDAAETVIIDTGCANLSSVRFAFERLGA
CCCHHHHHHHHHHCCCCCCHHEEECCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHCCC
KVLVTDDKAKIKAAKRVVLPGVGTAGAAMASLHEKGLVELIQGLTQPVLGVCLGMQMLAA
EEEEECCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLNVSNSPNVHPLFAGI
HHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEC
EDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQMLRNF
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
LQMDELQMNKALMNQVLQSSSADGQAGAKKADQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQDTQQSKLAQLSANEPQDTALAANSADAVTNDAAETVIIDTGCANLSSVRFAFERLGA
CCCHHHHHHHHHHCCCCCCHHEEECCCCCHHCCCCCCEEEEECCCCCHHHHHHHHHHCCC
KVLVTDDKAKIKAAKRVVLPGVGTAGAAMASLHEKGLVELIQGLTQPVLGVCLGMQMLAA
EEEEECCHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ISKERGGKGQDCECLGIIPTDICELDTQLLKAESLPLPHMGWNQLNVSNSPNVHPLFAGI
HHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCEEEEEC
EDGSYVYFVHSYRAPLSEFTLAECTYGEGFSAAIGKGNFMGVQFHPEKSAAVGAQMLRNF
CCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHH
LQMDELQMNKALMNQVLQSSSADGQAGAKKADQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]