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The map label for this gene is puuB [H]

Identifier: 126174634

GI number: 126174634

Start: 2818152

End: 2819441

Strand: Reverse

Name: puuB [H]

Synonym: Sbal_2421

Alternate gene names: 126174634

Gene position: 2819441-2818152 (Counterclockwise)

Preceding gene: 126174637

Following gene: 126174632

Centisome position: 54.99

GC content: 51.63

Gene sequence:

>1290_bases
ATGCCAGTAGAGCGTTGCGCCTCCTATTACAATGCCTCCATTAACCAAGAGTCGGATTATCCTGCGCTTGAAGGCGACAT
TCGCGTCGATGTGGCGATTGTCGGTGGCGGGTTTACCGGTGTAGCAACGGCGCTGGAATTGTCCGAGAAGGGCTACAAGG
TGGCACTTATTGAGGCTAATAAAATTGCGTGGGGCGCAACCGGACGCAACGGCGGGCAGGTGACCGGCAGTTTGTCTGGC
GATGCCGCGATGACAAAACAGCTGCGCCGTCAGATTGGCAATGAAGCTGAAGATTATGTGTGGAATTTACGTTGGCGCGG
CCATGACATTATTAAAAATCGGGTGGCCAAGTACGGCATAGACTGTGATTTAAAATTTGGTCATATCCAAACCGCTTACC
AGCCAAATCACATGCACGAGCTTAGGGCGATGCATGATGAAGCGCAGCGTCGCGGCATGGGGGAGTTTATGACGTTAGTC
TCTGCCGATGAAATGCCCGCCTATTTAGGCTCGCCCTTGTATCACGGCGGCTTAGTCAATCGGCGCAACATGCATTTACA
CTCAGTCAATCTATGCCTGGGTGAAGCCCGCGCCGCAGCAGGTCTTGGGGCGTTAATCTTTGAAAACTCGCCCGTGCTCG
ATATTGAAGAAGGCGATCTTTCTACCGTTGTCACCGCCAAGGGAAAAGTGCGCGCCAATAGCGTGCTGATCGCGGGGAAT
GCCTACCATAAACTCGCGCGTCCTCAACTGCGCGGCATGTTATTTCCAGCGTCCTTAGGCAATTGTGCTACTGCAATTTT
GCCTGATGAGATCGCTGCGCAAATCAATCCGCAGGATTTAGCCGTGTACGATTGTCGCTTTGTGCTGGATTACTATCGCC
TGACTGCAGATAAACGGTTGATGTTCGGCGGCGGCACTAATTACAGCGGCCGCGATCCTAAAAATGTGGAGGCGGAATTA
CGGCCTGCGATTGAGCGCACGTTTCCGCAATTAAAGGGGGTTGATATCGAATTTGCGTGGGCGGGAATGGCTGGGATTGT
GATTAATCGTATCCCGCAGCTCGGCAAAGTATCGCCTAATGTATTCTATTGCCAAGGATATTCAGGGCATGGGGTGGCGA
CATCACACATTATGGCTGAGATCATGGCCAATGCGATGGACGGACAATTGCGTGAATTTGATTTGTTTGCCGGCATGCGC
CATATCCGTATACCGTTAAATGAATGGTTTGGTAATCAAGCGCTGGCGTTAGGCATGTTGTATTACACTTTACGGGAAAA
CTGGCGCTAG

Upstream 100 bases:

>100_bases
AACTGAGTGTCCAGAATAACTGAGTGTCCAGAACTAAGACTTAACATTCACTTTCAGCAATCAACTTTCAACACTTAACT
TTCAACATCAAGGAATGACT

Downstream 100 bases:

>100_bases
TTTGCTGGGTGTTGTCTGTTATCGCTTCGTTTGTTCGAACTGAGTAATCCGCTCAACAACCGGCTGTTCAGAAAAAGATT
AGTCCTGTTCGATGAGTTTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPVERCASYYNASINQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPNHMHELRAMHDEAQRRGMGEFMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFENSPVLDIEEGDLSTVVTAKGKVRANSVLIAGN
AYHKLARPQLRGMLFPASLGNCATAILPDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVEAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMR
HIRIPLNEWFGNQALALGMLYYTLRENWR

Sequences:

>Translated_429_residues
MPVERCASYYNASINQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSG
DAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPNHMHELRAMHDEAQRRGMGEFMTLV
SADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFENSPVLDIEEGDLSTVVTAKGKVRANSVLIAGN
AYHKLARPQLRGMLFPASLGNCATAILPDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVEAEL
RPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMR
HIRIPLNEWFGNQALALGMLYYTLRENWR
>Mature_428_residues
PVERCASYYNASINQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEANKIAWGATGRNGGQVTGSLSGD
AAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGIDCDLKFGHIQTAYQPNHMHELRAMHDEAQRRGMGEFMTLVS
ADEMPAYLGSPLYHGGLVNRRNMHLHSVNLCLGEARAAAGLGALIFENSPVLDIEEGDLSTVVTAKGKVRANSVLIAGNA
YHKLARPQLRGMLFPASLGNCATAILPDEIAAQINPQDLAVYDCRFVLDYYRLTADKRLMFGGGTNYSGRDPKNVEAELR
PAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPNVFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMRH
IRIPLNEWFGNQALALGMLYYTLRENWR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=425, Percent_Identity=36.4705882352941, Blast_Score=293, Evalue=1e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47286; Mature: 47154

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVERCASYYNASINQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEAN
CCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYQPNHMHELRAMHDEAQRRGMGEFMTLVSADEMPAYLGSPLYHGGLVN
CEEEEECCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGALIFENSPVLDIEEGDLSTVVTAKGKVRANSVLIAGN
CCCCEEEEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCCEEEEEECCCEEECEEEEECC
AYHKLARPQLRGMLFPASLGNCATAILPDEIAAQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCCCEEEECCHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVEAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC
>Mature Secondary Structure 
PVERCASYYNASINQESDYPALEGDIRVDVAIVGGGFTGVATALELSEKGYKVALIEAN
CHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEC
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRQIGNEAEDYVWNLRWRGHDIIKNRVAKYGI
EEEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHEEEEEEECCHHHHHHHHHHCCC
DCDLKFGHIQTAYQPNHMHELRAMHDEAQRRGMGEFMTLVSADEMPAYLGSPLYHGGLVN
CEEEEECCEEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCC
RRNMHLHSVNLCLGEARAAAGLGALIFENSPVLDIEEGDLSTVVTAKGKVRANSVLIAGN
CCCCEEEEEEEEHHHHHHHCCCEEEEECCCCEEEECCCCCEEEEEECCCEEECEEEEECC
AYHKLARPQLRGMLFPASLGNCATAILPDEIAAQINPQDLAVYDCRFVLDYYRLTADKRL
HHHHHCCHHHHCEECCCCCCCCEEEECCHHHHHCCCCCCEEEEHHHHHHHHHHHCCCCEE
MFGGGTNYSGRDPKNVEAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKVSPN
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHCCCCCCCCCCC
VFYCQGYSGHGVATSHIMAEIMANAMDGQLREFDLFAGMRHIRIPLNEWFGNQALALGML
EEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEEEEHHHHCCCHHHHHHHH
YYTLRENWR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]