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The map label for this gene is ilvI [H]

Identifier: 126174599

GI number: 126174599

Start: 2777365

End: 2779083

Strand: Direct

Name: ilvI [H]

Synonym: Sbal_2386

Alternate gene names: 126174599

Gene position: 2777365-2779083 (Clockwise)

Preceding gene: 126174598

Following gene: 126174600

Centisome position: 54.17

GC content: 48.75

Gene sequence:

>1719_bases
ATGGAGATGTTATCCGGCGCCAGCATGATAGTGCGTTCTTTGATCGATGAAGGCGTAAAGCACATTTTTGGCTATCCTGG
CGGTTCAGTTTTAGATATCTACGACGCCCTGCACGTTATCCCCGGTATTGAACACATACTCGTTCGCCACGAACAAGCCG
CCGTACACATGGCCGATGGCTACGCCCGTGCCACGGGTAAAGTGGGCGTGGTATTAGTCACCTCAGGCCCAGGCGCGACC
AACGCGATTACCGGTATTGCCACCGCTTACATGGATTCTATCCCCCTTGTGGTGTTATCGGGTCAAGTCCCGAGCAATCT
TATCGGTAACGATGCCTTCCAAGAATGCGACATGATAGGTATCTCTCGCCCTGTAGTGAAACACAGCTTTTTAGTTAAAG
ATCCGACTGAAATTCCTGAAATTATTAAAAAGGCTTTTTACATTGCCTCTACTGGCCGTCCCGGTCCTGTGGTTGTCGAT
CTGCCAAAAGATTGTTTAAACCCCGCCCTGCTGCACGAATATTGCTACCCAGAAAGCGTCAAAATGCGCTCGTACAATCC
AACGACATCAGGCCATAAAGGTCAGATCCGCCGTGGTCTTCAAGCCCTGTTAGCCGCAAAAAAACCGGTACTTTATGTCG
GTGGCGGCGCAATTATCTCAGGTTGTGATAAGCAAATTCTGCAATTAGCTGAGAGATTAAACATTCCCGTCGTCAGCACG
TTGATGGGGCTTGGCGCCTTTCCAAGCACCCACAAAAACAGTTTAGGCATGCTTGGGATGCACGGTCGCTACGAAGCCAA
CATGACAATGCATAACTGCGATTTGATCTTCGGTATTGGTGTACGCTTCGACGATAGAACCACCAATAATGTGGAAAAAT
ACTGTCCGAACGCGACCATTTTGCACATAGATATCGATCCCTCTTCTATTTCGAAAACTATCCACGTGGATATTCCTATC
GTGGGGTCAGCCGACAGTATTTTAGACAGCATGTTGGCACAGTTAGATGAATCGAACGAACACAACGACAACGATGCTAT
TGCTACTTGGTGGAATGACATTGAACAATGGCGCAGTCGCGATTGTCTGGCCTATGACAAAAACAGTGGCCGCATCAAAC
CACAGCAAGTGATTGAAACCTTGTATAAGCTGACCAATGGCGATGCCTATGTCGCATCAGATGTTGGCCAGCACCAAATG
TTTGCCGCCTTGTACTATCCGTTCGATAAGCCGCGCCGCTGGATCAACTCAGGTGGCCTAGGGACAATGGGATTCGGCCT
GCCCGCCGCGATGGGCGTAAAAATGGCCATGCCTGATGCTACTGTTGTGTGTGTCACGGGTGATGGTTCCATCCAGATGA
ACATTCAAGAGCTTTCGACCGCGCTGCAATATGATACGCCGGTTAAAATCATCAACTTAAACAATCGTTTCCTTGGCATG
GTAAAACAATGGCAGGACATGATTTACTCTGGCCGTCACTCACATTCCTATATGGATTCAGTGCCAAATTTTGCCAAAAT
TGCCGAGGCCTATGGTCATGTTGGCATGACGATCAGCGATCCCGCCGAACTTGAGTCAAAAATGGCTGAAGCGCTGGCGA
TGAAAGACAGACTGGTCTTTATCGATATCATGGTGGATGAAACTGAGCACGTGTACCCCATGTTGATCCGCGGTGGTGCG
ATGAATGAAATGTGGCTGACCAAGACGGAGAAATGCTAA

Upstream 100 bases:

>100_bases
CGGAGTCTGAATTACCCTTCAGGCTTAATTTTTAGAGCGATTACCGTCGCTGTAACAAGATACTGTTTACCGTAACGGTA
CTATGCAATAGGAGTTCCAC

Downstream 100 bases:

>100_bases
TGCGTCGAATTATATCTGTATTATTAGAAAACCAATCCGGCGCCCTGTCTCGTGTGGTCGGTTTGTTTTCACAGCGCGGT
TATAACATTGAAAGTTTAAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLAQLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC

Sequences:

>Translated_572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLAQLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC
>Mature_572_residues
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADGYARATGKVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIGISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVD
LPKDCLNPALLHEYCYPESVKMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
LMGLGAFPSTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATILHIDIDPSSISKTIHVDIPI
VGSADSILDSMLAQLDESNEHNDNDAIATWWNDIEQWRSRDCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVFIDIMVDETEHVYPMLIRGGA
MNEMWLTKTEKC

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=592, Percent_Identity=26.8581081081081, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21361361, Length=511, Percent_Identity=25.440313111546, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=573, Percent_Identity=74.869109947644, Blast_Score=927, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=39.8576512455516, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1786717, Length=533, Percent_Identity=33.9587242026266, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787096, Length=555, Percent_Identity=28.8288288288288, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=24.2857142857143, Blast_Score=162, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17531299, Length=583, Percent_Identity=25.2144082332762, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=591, Percent_Identity=25.7191201353638, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=600, Percent_Identity=24.3333333333333, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=41.4089347079038, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=474, Percent_Identity=24.6835443037975, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320588, Length=528, Percent_Identity=23.1060606060606, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=522, Percent_Identity=22.6053639846743, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323073, Length=497, Percent_Identity=22.3340040241449, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6323163, Length=327, Percent_Identity=25.0764525993884, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922626, Length=594, Percent_Identity=22.5589225589226, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62638; Mature: 62638

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHEYCYPESV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCHHHHHHHHHHCCCCHHHH
LMGLGAFPSTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATI
HHHCCCCCCCCCCCCCEEECCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIHVDIPIVGSADSILDSMLAQLDESNEHNDNDAIATWWNDIEQWRSR
EEEECCHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
DCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGGL
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLTKTEKC
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MEMLSGASMIVRSLIDEGVKHIFGYPGGSVLDIYDALHVIPGIEHILVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHEEEECCC
YARATGKVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVPSNLIGNDAFQECDMIG
CEECCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCC
ISRPVVKHSFLVKDPTEIPEIIKKAFYIASTGRPGPVVVDLPKDCLNPALLHEYCYPESV
CCCCHHHHEEEECCCHHHHHHHHHHEEEEECCCCCCEEEECCHHHCCHHHHHHHCCCCCE
KMRSYNPTTSGHKGQIRRGLQALLAAKKPVLYVGGGAIISGCDKQILQLAERLNIPVVST
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHCHHHHHHHHHHCCCCHHHH
LMGLGAFPSTHKNSLGMLGMHGRYEANMTMHNCDLIFGIGVRFDDRTTNNVEKYCPNATI
HHHCCCCCCCCCCCCCEEECCCCEECCEEEEECEEEEEECEEECCCCCCCHHHHCCCCEE
LHIDIDPSSISKTIHVDIPIVGSADSILDSMLAQLDESNEHNDNDAIATWWNDIEQWRSR
EEEECCHHHCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
DCLAYDKNSGRIKPQQVIETLYKLTNGDAYVASDVGQHQMFAALYYPFDKPRRWINSGGL
CCEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHEEHHEECCCHHHHHHHCCCCC
GTMGFGLPAAMGVKMAMPDATVVCVTGDGSIQMNIQELSTALQYDTPVKIINLNNRFLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHCCCCCEEEEECCCHHHHH
VKQWQDMIYSGRHSHSYMDSVPNFAKIAEAYGHVGMTISDPAELESKMAEALAMKDRLVF
HHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHCCCEEE
IDIMVDETEHVYPMLIRGGAMNEMWLTKTEKC
EEEEECCCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]