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The map label for this gene is ptsI [H]

Identifier: 126174574

GI number: 126174574

Start: 2747717

End: 2749420

Strand: Direct

Name: ptsI [H]

Synonym: Sbal_2361

Alternate gene names: 126174574

Gene position: 2747717-2749420 (Clockwise)

Preceding gene: 126174573

Following gene: 126174575

Centisome position: 53.59

GC content: 49.53

Gene sequence:

>1704_bases
ATGTCAATAACGGGGATTATAGTGTCATCGGGAATAGCCTTTGGTCAGGCACTTCACCTTATTCACACCGAACACCACCT
CGATTATCGCCCTATTCCCCTGTCGAAAATTCCCCAACAACAGGGCAAGTTTGCCAAAGCCTTGCAAGAGCTGCTGGCAC
AATTAACCCACAGCCAAGCTGCGCTCGATAGCGATTCAGAAAATTATCAACTGATCGAAGCCGATTTATTGTTATTGGAA
GACGATGAATTAATCGAGCAAGTGAACGATGCGATTCGTACCTTACAACTGTCGGCAAGTGTGGCGGTTGAACGCATATT
TGCCCATCAAGCCAACGAGTTACAATCCCTAGATGATCCCTATTTAGCTAATCGAGCCCAAGATGTACGCTGTTTAGGCC
AACGCGTTGTCGCCGCGATCAATGGCCATTTAAACCAAGGGCTCGAAAAACTCGACAGACCCACCATCTTGTTGGCACAA
GATTTAACCCCCGCCGAATTTGCCTTGCTGCCGAGGGAAAACCTCTGCGGTATTGTGCTCAAAACTGGCGGTTTAACCAG
TCATACGGCGATTTTAGCCCGAGCTGCCGGCATTCCAGCCATCTTAAGTTGTCAGTTTGATGCCGATTCGATCCCCAACG
GCACGCCCTTAGTACTAGATGCGCTCAATGGTGAGCTTTGCGTTAAGCCCAATCCAGATCAACAGGCAAGACTCACAGTC
ACCTTTCACCACGAACAGGCAAGACGGGCGGCGCTGCAAACCTACAAAGATGGCCCAGCGCAAACCCAAGATGGCCATAT
CGTGGGGCTTATGGCTAACGTGGGTAATCTCAACGACATCACCCATGTCAGCGATGTTGGCGCCGATGGTATAGGTTTGT
TTCGCACTGAATTTATGCTGATGAACGTCAGCACCCTGCCCGATGAGAAAGCCCAATACAGCTTATATTGCGATGCATTG
CACGCTCTGGGCGGTAAGACCTTTACCATCCGCACCTTAGATATCGGTGCCGATAAAGAACTGCCTTGCCTGTGCCAAGA
AATAGAAGACAATCCCGCCTTAGGGCTGCGCGGCATTCGCTACACTTTGGCACACCCCGACTTATTTAAAACCCAATTGA
GGGCCATTCTGCGCGCCGCAAACCATGGCCCAATCCGCTTGATGTTCCCTATGGTCAATCAAGTCGAAGAATTGGATGAA
GTGTTTGCACTGATTGCCCAGTGCCAAGATGCCCTGGAAGAAGAAGAGAAAGGCTACGGTGAACTCAGCTACGGTATCGT
TGTCGAAACCCCCGCAGCGGTATTTAACCTCAATGCTATGCTGCCACGACTCGACTTTGTCAGCATTGGCACCAATGATT
TAACCCAATATGCAATGGCAGCCGATAGGACCAATCCGCAGCTTACCCGCGACTATCCGTCCCTTTCGCCTGCCATTTTA
GCGTTAATTAACATGACAATAGTCCAAGCAAAAGCGGCCAATGTGAAAGTGTCGCTGTGCGGCGAACTGGCCAGTTCACC
ACAAATTGTACCGCTGTTAATCGGCATGGGGCTGGACGAACTCAGTGTTAACTTAAGCTCACTGTTAGAAGTCAAAGCTG
CCATTTGCCAAGGCAACATCCAACAATTTTCGGCGCTGGCGCACACTGCATTACAACAAGATAGAATTGCAGGTCTACAG
CAGTGTATAACAAGCTATAAATAG

Upstream 100 bases:

>100_bases
CCAATAGTTTACATGCCAACGCTTTACAAACTCAACGCCACTCAACTAGACTCGCTAGCGCTAACGGGCACTCAGTTCAG
TGGTTAATTTAGGAAGGGAT

Downstream 100 bases:

>100_bases
AGACATTTATTCGTTATGCTGCATTACAGAACAAAAACAATGACAAAAACAAGCAAAAGAGGCTCTAGATTATGGGATTT
TTAAGCCGAATTAGGCGCTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELLAQLTHSQAALDSDSENYQLIEADLLLLE
DDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQ
DLTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVKPNPDQQARLTV
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDAL
HALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDE
VFALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
ALINMTIVQAKAANVKVSLCGELASSPQIVPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQ
QCITSYK

Sequences:

>Translated_567_residues
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELLAQLTHSQAALDSDSENYQLIEADLLLLE
DDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQ
DLTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVKPNPDQQARLTV
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDAL
HALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDE
VFALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
ALINMTIVQAKAANVKVSLCGELASSPQIVPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQ
QCITSYK
>Mature_566_residues
SITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELLAQLTHSQAALDSDSENYQLIEADLLLLED
DELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDPYLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQD
LTPAEFALLPRENLCGIVLKTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVKPNPDQQARLTVT
FHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFMLMNVSTLPDEKAQYSLYCDALH
ALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIRYTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEV
FALIAQCQDALEEEEKGYGELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAILA
LINMTIVQAKAANVKVSLCGELASSPQIVPLLIGMGLDELSVNLSSLLEVKAAICQGNIQQFSALAHTALQQDRIAGLQQ
CITSYK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=554, Percent_Identity=37.3646209386282, Blast_Score=344, Evalue=1e-95,
Organism=Escherichia coli, GI1789193, Length=461, Percent_Identity=37.0932754880694, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI1788726, Length=569, Percent_Identity=32.5131810193322, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI48994992, Length=497, Percent_Identity=33.8028169014084, Blast_Score=258, Evalue=9e-70,
Organism=Escherichia coli, GI1787994, Length=451, Percent_Identity=23.059866962306, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 61586; Mature: 61455

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELLAQLTHSQA
CCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHH
ALDSDSENYQLIEADLLLLEDDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDP
HCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQDLTPAEFALLPRENLCGIVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHEEECCCCCCCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVKPNPDQQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEE
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHEEE
MNVSTLPDEKAQYSLYCDALHALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIR
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCEE
YTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEVFALIAQCQDALEEEEKGYG
EEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
EEEECEEEECCHHHHEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ALINMTIVQAKAANVKVSLCGELASSPQIVPLLIGMGLDELSVNLSSLLEVKAAICQGNI
HHHHHHHHEECCCCEEEEEHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
QQFSALAHTALQQDRIAGLQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITGIIVSSGIAFGQALHLIHTEHHLDYRPIPLSKIPQQQGKFAKALQELLAQLTHSQA
CEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHCCHHCCHHHHHHHHHHHHHHHHHH
ALDSDSENYQLIEADLLLLEDDELIEQVNDAIRTLQLSASVAVERIFAHQANELQSLDDP
HCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YLANRAQDVRCLGQRVVAAINGHLNQGLEKLDRPTILLAQDLTPAEFALLPRENLCGIVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHEEECCCCCCCEEEE
KTGGLTSHTAILARAAGIPAILSCQFDADSIPNGTPLVLDALNGELCVKPNPDQQARLTV
ECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCEEEEE
TFHHEQARRAALQTYKDGPAQTQDGHIVGLMANVGNLNDITHVSDVGADGIGLFRTEFML
EEEHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCHHHHHHEEE
MNVSTLPDEKAQYSLYCDALHALGGKTFTIRTLDIGADKELPCLCQEIEDNPALGLRGIR
EECCCCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCCCCCCCCCEE
YTLAHPDLFKTQLRAILRAANHGPIRLMFPMVNQVEELDEVFALIAQCQDALEEEEKGYG
EEECCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ELSYGIVVETPAAVFNLNAMLPRLDFVSIGTNDLTQYAMAADRTNPQLTRDYPSLSPAIL
EEEECEEEECCHHHHEHHHHCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
ALINMTIVQAKAANVKVSLCGELASSPQIVPLLIGMGLDELSVNLSSLLEVKAAICQGNI
HHHHHHHHEECCCCEEEEEHHHHCCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHCCCH
QQFSALAHTALQQDRIAGLQQCITSYK
HHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]