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The map label for this gene is slt [H]

Identifier: 126174489

GI number: 126174489

Start: 2630649

End: 2632574

Strand: Direct

Name: slt [H]

Synonym: Sbal_2273

Alternate gene names: 126174489

Gene position: 2630649-2632574 (Clockwise)

Preceding gene: 126174488

Following gene: 126174490

Centisome position: 51.31

GC content: 48.55

Gene sequence:

>1926_bases
ATGTGCAAAGCCGTGATGAATGTGGTTATATCCAGCGCAATATTGCTCGCAGGCGCCTTGAATGCCCCTTCTGCATTCGC
GTTAACGCCAACGCAGAAAACCTTCCTTGAGGCCGAAAAAGCCTTAAAGAAACAAGACTATGAAGTCTATAAACCCCTAC
GGGCAAAATTGGGCGATTATCCGCTGGCGATTTACTTAGACCATGATATCGACATAGGCGATCTCAATGGTTTGCATGGC
GCTGCGGCAAAAAATCTGATTGATAAGTATGAAACTACGCCTATGTACAACAGGTTAAGGTCTAAATACTTAAACAATGC
GGGCACCCAAAAACGCTGGAGTGATTATCTTGCGATAAGCCCCGATGTCCCTACCGACATCCGCCTGCAATGTTATTACT
ACGAAGCAAAACTTGCCAAGGGCGAGATAAAAACCGCCTATGCGGCGGCGCAATCACTCTGGGTCTTTGGTAGTTCACGG
CCAAAAGAGTGTGATCCCCTGTTCAACGCTTGGACCAAAGCGGGTAAACGTACTCAAGATGTGATTTGGGCGCGGATGAT
GCTGAGTTTTGAGACTGGCGAAACCAGTCTGCTGAGCTATTTATCCCAGAAAATCACCACCCATAATAACGAAGCGAAGC
GCTTCGTCGCCGTCTATAAAGATCCAAACAGCCTGCGCCATACCGAAAAGTTTAAGGATAAAAATCCGATTGTGGGTGAC
ATAGTCGCGGCGGGATTAAAACGTCTTGCCCGCAAGGATCTCGACCAAGCGATTAATTTGTATGAACGGTATCAAAAGGC
TAATCGTTTCACGCCAGCACAAGCCCAACAACTGGATAAATACTTAGTTCGCCGCATCTTAATCGAACAAGATGATAGCC
ATAAAAGCTATGTCGACAATGTGCTGGCCGAGCTGAAAAGTGACGACTTATTTGAACGCCGGTTAAGATGGGCCATTCGC
GATCACGACATGCGCAGCATTGCCCGTTATTTAAATTTACTGCAACCTGAAACCTTGGCTAAGGAGCGCTGGCAATATTG
GCTCTATCGCACTAATGCTAAAGATGCGCCAGAAAGTGCCACTAAGGCTCTTGCGACAATCAGCAATGAACGTAATTTTT
ACGGTTTTGCCGCCGCGCAGTTTTTAAATAAACCCGTCTCGCTGAATCAAAGCCCTGAGCCTATCGTCGACGCCAGCAGC
CAAAAATTAACCGATGACCTGGGTTTTGTGCGCGTGCAAGAGCTAATGGCGCTGGATCGTTATTTCGATGCACGTTACGA
ATGGATGTCGCTGCTCAGACGCAGTGACAACACTATGCGTGCCCGTTACGGCCGTTATGCCCACGAGCAAGGCTGGTATG
ATTTTGGCGTCGAAGCCAGTATCCAAGGTAAGTTGTGGGATGACATTCCCCTACGCTTCCCGATGGCGCACCAAGAAGGT
TTTGAGCACGCCAGTAAAAAACACAAAGTGAACATAGATGAGATTCGAGCGATCAGTCGCCGCGAGAGCGCGTTTTATCT
TTATGCGACCTCAGGCGTGGGTGCCCGCGGCTTAATGCAAATTATGCCTGCGACCGCTAAAGCAACCGCCAAGAAACATG
GGGCTAAATATAGCGATCCTAAGGATCTTTATAGCGCAGAGCTTAACCTCAATCTTGGCAGCGCTTACTATGCCCAGTTG
TTAAAAGAGTTTAACCAGAACCGTATCCTCGCCACAGCCGCCTATAACGCGGGCCCATCACGGGTGCGTCGCTGGTTGGC
AAACTCGGATGGAAAACTCGATGCCATGGGCTTTATCGAAGCCATTCCCTTCACTGAGACCCGTGAATATGTGCAAGCCG
TCTTCAGCTATCGATTGATTTATGAGGCGCAGGAGCAAAAAGCGCAGCCATTATTCAGCGAAGCCGAACTGAAGTTTGCT
TACTAA

Upstream 100 bases:

>100_bases
TGATAGACCTAATTGATTGATAGAGTTGAACACTCGCCAATAACCTTGAATCGATACGGACATGTCGATTGATATCTTGA
TGCCCGGAGGCAAACGCTCC

Downstream 100 bases:

>100_bases
CTCGCGACATAAAAAGGAACTCGATGCTGCTGAGCATCGAGTTCTTACTTTTCAGCATACCCTAGGGTAATGCTCATGCT
TTAACCGCCAAAAATTCTAA

Product: lytic transglycosylase catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MCKAVMNVVISSAILLAGALNAPSAFALTPTQKTFLEAEKALKKQDYEVYKPLRAKLGDYPLAIYLDHDIDIGDLNGLHG
AAAKNLIDKYETTPMYNRLRSKYLNNAGTQKRWSDYLAISPDVPTDIRLQCYYYEAKLAKGEIKTAYAAAQSLWVFGSSR
PKECDPLFNAWTKAGKRTQDVIWARMMLSFETGETSLLSYLSQKITTHNNEAKRFVAVYKDPNSLRHTEKFKDKNPIVGD
IVAAGLKRLARKDLDQAINLYERYQKANRFTPAQAQQLDKYLVRRILIEQDDSHKSYVDNVLAELKSDDLFERRLRWAIR
DHDMRSIARYLNLLQPETLAKERWQYWLYRTNAKDAPESATKALATISNERNFYGFAAAQFLNKPVSLNQSPEPIVDASS
QKLTDDLGFVRVQELMALDRYFDARYEWMSLLRRSDNTMRARYGRYAHEQGWYDFGVEASIQGKLWDDIPLRFPMAHQEG
FEHASKKHKVNIDEIRAISRRESAFYLYATSGVGARGLMQIMPATAKATAKKHGAKYSDPKDLYSAELNLNLGSAYYAQL
LKEFNQNRILATAAYNAGPSRVRRWLANSDGKLDAMGFIEAIPFTETREYVQAVFSYRLIYEAQEQKAQPLFSEAELKFA
Y

Sequences:

>Translated_641_residues
MCKAVMNVVISSAILLAGALNAPSAFALTPTQKTFLEAEKALKKQDYEVYKPLRAKLGDYPLAIYLDHDIDIGDLNGLHG
AAAKNLIDKYETTPMYNRLRSKYLNNAGTQKRWSDYLAISPDVPTDIRLQCYYYEAKLAKGEIKTAYAAAQSLWVFGSSR
PKECDPLFNAWTKAGKRTQDVIWARMMLSFETGETSLLSYLSQKITTHNNEAKRFVAVYKDPNSLRHTEKFKDKNPIVGD
IVAAGLKRLARKDLDQAINLYERYQKANRFTPAQAQQLDKYLVRRILIEQDDSHKSYVDNVLAELKSDDLFERRLRWAIR
DHDMRSIARYLNLLQPETLAKERWQYWLYRTNAKDAPESATKALATISNERNFYGFAAAQFLNKPVSLNQSPEPIVDASS
QKLTDDLGFVRVQELMALDRYFDARYEWMSLLRRSDNTMRARYGRYAHEQGWYDFGVEASIQGKLWDDIPLRFPMAHQEG
FEHASKKHKVNIDEIRAISRRESAFYLYATSGVGARGLMQIMPATAKATAKKHGAKYSDPKDLYSAELNLNLGSAYYAQL
LKEFNQNRILATAAYNAGPSRVRRWLANSDGKLDAMGFIEAIPFTETREYVQAVFSYRLIYEAQEQKAQPLFSEAELKFA
Y
>Mature_641_residues
MCKAVMNVVISSAILLAGALNAPSAFALTPTQKTFLEAEKALKKQDYEVYKPLRAKLGDYPLAIYLDHDIDIGDLNGLHG
AAAKNLIDKYETTPMYNRLRSKYLNNAGTQKRWSDYLAISPDVPTDIRLQCYYYEAKLAKGEIKTAYAAAQSLWVFGSSR
PKECDPLFNAWTKAGKRTQDVIWARMMLSFETGETSLLSYLSQKITTHNNEAKRFVAVYKDPNSLRHTEKFKDKNPIVGD
IVAAGLKRLARKDLDQAINLYERYQKANRFTPAQAQQLDKYLVRRILIEQDDSHKSYVDNVLAELKSDDLFERRLRWAIR
DHDMRSIARYLNLLQPETLAKERWQYWLYRTNAKDAPESATKALATISNERNFYGFAAAQFLNKPVSLNQSPEPIVDASS
QKLTDDLGFVRVQELMALDRYFDARYEWMSLLRRSDNTMRARYGRYAHEQGWYDFGVEASIQGKLWDDIPLRFPMAHQEG
FEHASKKHKVNIDEIRAISRRESAFYLYATSGVGARGLMQIMPATAKATAKKHGAKYSDPKDLYSAELNLNLGSAYYAQL
LKEFNQNRILATAAYNAGPSRVRRWLANSDGKLDAMGFIEAIPFTETREYVQAVFSYRLIYEAQEQKAQPLFSEAELKFA
Y

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=628, Percent_Identity=30.8917197452229, Blast_Score=318, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 73423; Mature: 73423

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKAVMNVVISSAILLAGALNAPSAFALTPTQKTFLEAEKALKKQDYEVYKPLRAKLGDY
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
PLAIYLDHDIDIGDLNGLHGAAAKNLIDKYETTPMYNRLRSKYLNNAGTQKRWSDYLAIS
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCEEEC
PDVPTDIRLQCYYYEAKLAKGEIKTAYAAAQSLWVFGSSRPKECDPLFNAWTKAGKRTQD
CCCCCCEEEEEEEEEEHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHH
VIWARMMLSFETGETSLLSYLSQKITTHNNEAKRFVAVYKDPNSLRHTEKFKDKNPIVGD
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHH
IVAAGLKRLARKDLDQAINLYERYQKANRFTPAQAQQLDKYLVRRILIEQDDSHKSYVDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VLAELKSDDLFERRLRWAIRDHDMRSIARYLNLLQPETLAKERWQYWLYRTNAKDAPESA
HHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHEEEEECCCCCCHHHH
TKALATISNERNFYGFAAAQFLNKPVSLNQSPEPIVDASSQKLTDDLGFVRVQELMALDR
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YFDARYEWMSLLRRSDNTMRARYGRYAHEQGWYDFGVEASIQGKLWDDIPLRFPMAHQEG
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCEECCCCEEECCCCCCCCCCCCCCHHHHH
FEHASKKHKVNIDEIRAISRRESAFYLYATSGVGARGLMQIMPATAKATAKKHGAKYSDP
HHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCCH
KDLYSAELNLNLGSAYYAQLLKEFNQNRILATAAYNAGPSRVRRWLANSDGKLDAMGFIE
HHHHEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCEEHHHHHH
AIPFTETREYVQAVFSYRLIYEAQEQKAQPLFSEAELKFAY
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECC
>Mature Secondary Structure
MCKAVMNVVISSAILLAGALNAPSAFALTPTQKTFLEAEKALKKQDYEVYKPLRAKLGDY
CHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCC
PLAIYLDHDIDIGDLNGLHGAAAKNLIDKYETTPMYNRLRSKYLNNAGTQKRWSDYLAIS
CEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHCCCEEEC
PDVPTDIRLQCYYYEAKLAKGEIKTAYAAAQSLWVFGSSRPKECDPLFNAWTKAGKRTQD
CCCCCCEEEEEEEEEEHHCCCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHCCCHHHH
VIWARMMLSFETGETSLLSYLSQKITTHNNEAKRFVAVYKDPNSLRHTEKFKDKNPIVGD
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHCCCCCCHHH
IVAAGLKRLARKDLDQAINLYERYQKANRFTPAQAQQLDKYLVRRILIEQDDSHKSYVDN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHH
VLAELKSDDLFERRLRWAIRDHDMRSIARYLNLLQPETLAKERWQYWLYRTNAKDAPESA
HHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHEEEEECCCCCCHHHH
TKALATISNERNFYGFAAAQFLNKPVSLNQSPEPIVDASSQKLTDDLGFVRVQELMALDR
HHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
YFDARYEWMSLLRRSDNTMRARYGRYAHEQGWYDFGVEASIQGKLWDDIPLRFPMAHQEG
HHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCEECCCCEEECCCCCCCCCCCCCCHHHHH
FEHASKKHKVNIDEIRAISRRESAFYLYATSGVGARGLMQIMPATAKATAKKHGAKYSDP
HHHHHHHCCCCHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCCH
KDLYSAELNLNLGSAYYAQLLKEFNQNRILATAAYNAGPSRVRRWLANSDGKLDAMGFIE
HHHHEEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCCCCCEEHHHHHH
AIPFTETREYVQAVFSYRLIYEAQEQKAQPLFSEAELKFAY
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]