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The map label for this gene is gcvA [H]

Identifier: 126174483

GI number: 126174483

Start: 2624316

End: 2625224

Strand: Reverse

Name: gcvA [H]

Synonym: Sbal_2267

Alternate gene names: 126174483

Gene position: 2625224-2624316 (Counterclockwise)

Preceding gene: 126174486

Following gene: 126174482

Centisome position: 51.2

GC content: 48.29

Gene sequence:

>909_bases
ATGTCTATTCGTCTTCCTCTTCTTTCCGCGTTACGCGCTTTTACGGCTCTTGCCCGTTTGGGCAGTATCAATGCTGCGGC
CGAAGAACTCAGTCTGACCCAAAGTGCGATCAGTCATCAAATTCGTTCGATCGAAGATTATCTCGATGTAGTTTTATTTA
ACCGCGATGGTCGAAAAATGACGTTGACCGATGCGGGGCGAGTGTATGCCTATCAAATCCGTCAAGCGTTAGATGATATT
GGTAGCGCCACCGATAAGCTCAGCCGTAAACCCAAACCAAATCAACTGGTCGTTTCTGTCTTACCGTCATTTGCGCAGTA
TTGGCTACTACCGCGATTAGCGGGTTTTGCGTTGCAGTATCCTGATTTTTATTTGACCTTATCGGCCACTATGGCTTTTT
CTAATTTTAATGATGATCCAATCGACTGTGCCATTCGCTTTGGTCACGGTGATTGGCCTGACTTACATTGCGAACTGTTG
ATGGGGGATTCATTATTACTAGTTGCTTCACCCGATTTTAATCAAGGCCAACTACCACAAACCGTTGCGCAAGTGATGCA
GGCACCATTACTGCACGCCAGTGAAAGCTGGGCTGTGTGGCTGTCGGCCGCAGGAGTTGAGCAATTGCGCCCCAAAGGGA
AGTTGGAGTTCACAGACTCGACTCATTTGCTTGAGGCCGCACGTTTGGGACTGGGTATAGCGCTTACTCGCCGTTCGATT
GCTCAAACATTGATTGACCGGGGTGAGTTAGTGCGCGTAACGGAAATCGAGCCTACCCATCCATCCGCAAACTACTACTT
AGTATGGCCTCATCGCGTGAATCAAACTCCGGCATTGTTGCAGTTAACAGCTTGGTTGCATGTTGAGGTGAGCCGCTATC
AGCAGGTTTATGATGCGAAAAAGCAGTAA

Upstream 100 bases:

>100_bases
AAAATATTCTAGATGACACGTGTCACCCTGCCTGCATTATATGAGTGTTTAAAATCGGTTGCTGCGCATAATTTTTGTCA
TTTAGCTGAATGGTTTTCAT

Downstream 100 bases:

>100_bases
AAAGTATAATAATCAACATTTTGAATTGTTTTTTAAAGTATTTACTTATGGGAACCAATAATACGTTTAAGATTTAAAAA
GGAATTTAAGATGTTGTTTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSIRLPLLSALRAFTALARLGSINAAAEELSLTQSAISHQIRSIEDYLDVVLFNRDGRKMTLTDAGRVYAYQIRQALDDI
GSATDKLSRKPKPNQLVVSVLPSFAQYWLLPRLAGFALQYPDFYLTLSATMAFSNFNDDPIDCAIRFGHGDWPDLHCELL
MGDSLLLVASPDFNQGQLPQTVAQVMQAPLLHASESWAVWLSAAGVEQLRPKGKLEFTDSTHLLEAARLGLGIALTRRSI
AQTLIDRGELVRVTEIEPTHPSANYYLVWPHRVNQTPALLQLTAWLHVEVSRYQQVYDAKKQ

Sequences:

>Translated_302_residues
MSIRLPLLSALRAFTALARLGSINAAAEELSLTQSAISHQIRSIEDYLDVVLFNRDGRKMTLTDAGRVYAYQIRQALDDI
GSATDKLSRKPKPNQLVVSVLPSFAQYWLLPRLAGFALQYPDFYLTLSATMAFSNFNDDPIDCAIRFGHGDWPDLHCELL
MGDSLLLVASPDFNQGQLPQTVAQVMQAPLLHASESWAVWLSAAGVEQLRPKGKLEFTDSTHLLEAARLGLGIALTRRSI
AQTLIDRGELVRVTEIEPTHPSANYYLVWPHRVNQTPALLQLTAWLHVEVSRYQQVYDAKKQ
>Mature_301_residues
SIRLPLLSALRAFTALARLGSINAAAEELSLTQSAISHQIRSIEDYLDVVLFNRDGRKMTLTDAGRVYAYQIRQALDDIG
SATDKLSRKPKPNQLVVSVLPSFAQYWLLPRLAGFALQYPDFYLTLSATMAFSNFNDDPIDCAIRFGHGDWPDLHCELLM
GDSLLLVASPDFNQGQLPQTVAQVMQAPLLHASESWAVWLSAAGVEQLRPKGKLEFTDSTHLLEAARLGLGIALTRRSIA
QTLIDRGELVRVTEIEPTHPSANYYLVWPHRVNQTPALLQLTAWLHVEVSRYQQVYDAKKQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=38.9763779527559, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=30.2721088435374, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=31.7567567567568, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI157672245, Length=163, Percent_Identity=34.3558282208589, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081978, Length=289, Percent_Identity=28.719723183391, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=249, Percent_Identity=27.3092369477912, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787128, Length=273, Percent_Identity=26.3736263736264, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787589, Length=277, Percent_Identity=28.158844765343, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=26.3513513513513, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789639, Length=257, Percent_Identity=25.2918287937743, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786508, Length=69, Percent_Identity=50.7246376811594, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081904, Length=201, Percent_Identity=31.8407960199005, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788748, Length=175, Percent_Identity=25.7142857142857, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33739; Mature: 33608

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRLPLLSALRAFTALARLGSINAAAEELSLTQSAISHQIRSIEDYLDVVLFNRDGRKM
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
TLTDAGRVYAYQIRQALDDIGSATDKLSRKPKPNQLVVSVLPSFAQYWLLPRLAGFALQY
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEC
PDFYLTLSATMAFSNFNDDPIDCAIRFGHGDWPDLHCELLMGDSLLLVASPDFNQGQLPQ
CCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCHH
TVAQVMQAPLLHASESWAVWLSAAGVEQLRPKGKLEFTDSTHLLEAARLGLGIALTRRSI
HHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCEEECCCHHHHHHHHHCCHHHHHHHHH
AQTLIDRGELVRVTEIEPTHPSANYYLVWPHRVNQTPALLQLTAWLHVEVSRYQQVYDAK
HHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHC
KQ
CC
>Mature Secondary Structure 
SIRLPLLSALRAFTALARLGSINAAAEELSLTQSAISHQIRSIEDYLDVVLFNRDGRKM
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEE
TLTDAGRVYAYQIRQALDDIGSATDKLSRKPKPNQLVVSVLPSFAQYWLLPRLAGFALQY
EEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEC
PDFYLTLSATMAFSNFNDDPIDCAIRFGHGDWPDLHCELLMGDSLLLVASPDFNQGQLPQ
CCEEEEEEEEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCCHH
TVAQVMQAPLLHASESWAVWLSAAGVEQLRPKGKLEFTDSTHLLEAARLGLGIALTRRSI
HHHHHHHHHHHCCCCCEEEEEECCCHHHHCCCCCEEECCCHHHHHHHHHCCHHHHHHHHH
AQTLIDRGELVRVTEIEPTHPSANYYLVWPHRVNQTPALLQLTAWLHVEVSRYQQVYDAK
HHHHHCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCHHEEEEHHHHHHHHHHHHHHHHC
KQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]