Click here to switch to the map view.

The map label for this gene is eda [H]

Identifier: 126174454

GI number: 126174454

Start: 2587858

End: 2588499

Strand: Reverse

Name: eda [H]

Synonym: Sbal_2238

Alternate gene names: 126174454

Gene position: 2588499-2587858 (Counterclockwise)

Preceding gene: 126174455

Following gene: 126174450

Centisome position: 50.48

GC content: 49.53

Gene sequence:

>642_bases
ATGCTTGAGAATAACTGGTCACTACAGCCACAAGATATTTTTAAACGCAGCCCTATTGTTCCTGTTATGGTGATTAACAA
GATTGAACATGCGGTACCATTAGCGCGAGCACTGGTTGCTGGCGGCATTAGCGTGTTAGAAGTGACTTTGCGTACACCTT
GCGCACTCGAAGCGATCACTAAAATAGCCAAAGAAGTGCCAGAGGCCTTAGTGGGCGCGGGTACTATCTTAAACGAAGCT
CAGCTTGATCTTGCGATTGCCGCTGGTGCGCAGTTTATTATTACCCCAGGCGCGACGGTTGAACTGCTCAAAGCCGGTAT
GCATGGTCCTGTACCATTGATCCCAGGCGTTGCGAGCATTTCAGAAGTGATGACGGGGATGGCGCTTGGTTACACCCACT
TTAAATTTTTCCCAGCCGAAGCCTCGGGTGGTGTGGATGCACTGAAAGCCTTCTCGGGTCCGCTAGCGGATATTCGCTTC
TGCCCAACAGGTGGCATTACGCCAAGCAGCTATAAAGATTATTTAGCGCTGAAAAACGTCGATTGTATTGGTGGCAGCTG
GATTGCGCCAACGGATGCAATGGAACACGGCGATTGGGATCGTATCACTCAACTTTGTAAAGATGCCATCGGCGCTTTAT
AA

Upstream 100 bases:

>100_bases
TATTTAGTGCGCTACGTTCCAATCTAAGCAGTCCAGAAACTGGTGCGCGTTGTACTGATGCCATCGATGAACTTTATTAA
TACAAAGGAAGAATAACGCA

Downstream 100 bases:

>100_bases
GTCGAGCTTGAAATAGCGACAAAAATAAAAAACCACCGTAAGGTGGTTTTTTGTCTTTCATCTTTAGCCTTTGATAAAGA
ATGTCTGCGATAGCTGGCGC

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MLENNWSLQPQDIFKRSPIVPVMVINKIEHAVPLARALVAGGISVLEVTLRTPCALEAITKIAKEVPEALVGAGTILNEA
QLDLAIAAGAQFIITPGATVELLKAGMHGPVPLIPGVASISEVMTGMALGYTHFKFFPAEASGGVDALKAFSGPLADIRF
CPTGGITPSSYKDYLALKNVDCIGGSWIAPTDAMEHGDWDRITQLCKDAIGAL

Sequences:

>Translated_213_residues
MLENNWSLQPQDIFKRSPIVPVMVINKIEHAVPLARALVAGGISVLEVTLRTPCALEAITKIAKEVPEALVGAGTILNEA
QLDLAIAAGAQFIITPGATVELLKAGMHGPVPLIPGVASISEVMTGMALGYTHFKFFPAEASGGVDALKAFSGPLADIRF
CPTGGITPSSYKDYLALKNVDCIGGSWIAPTDAMEHGDWDRITQLCKDAIGAL
>Mature_213_residues
MLENNWSLQPQDIFKRSPIVPVMVINKIEHAVPLARALVAGGISVLEVTLRTPCALEAITKIAKEVPEALVGAGTILNEA
QLDLAIAAGAQFIITPGATVELLKAGMHGPVPLIPGVASISEVMTGMALGYTHFKFFPAEASGGVDALKAFSGPLADIRF
CPTGGITPSSYKDYLALKNVDCIGGSWIAPTDAMEHGDWDRITQLCKDAIGAL

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=207, Percent_Identity=60.8695652173913, Blast_Score=256, Evalue=8e-70,
Organism=Escherichia coli, GI48994954, Length=160, Percent_Identity=27.5, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22375; Mature: 22375

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1 ; PS00160 ALDOLASE_KDPG_KHG_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENNWSLQPQDIFKRSPIVPVMVINKIEHAVPLARALVAGGISVLEVTLRTPCALEAIT
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHH
KIAKEVPEALVGAGTILNEAQLDLAIAAGAQFIITPGATVELLKAGMHGPVPLIPGVASI
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCCCCHHHH
SEVMTGMALGYTHFKFFPAEASGGVDALKAFSGPLADIRFCPTGGITPSSYKDYLALKNV
HHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHCCC
DCIGGSWIAPTDAMEHGDWDRITQLCKDAIGAL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLENNWSLQPQDIFKRSPIVPVMVINKIEHAVPLARALVAGGISVLEVTLRTPCALEAIT
CCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHH
KIAKEVPEALVGAGTILNEAQLDLAIAAGAQFIITPGATVELLKAGMHGPVPLIPGVASI
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEECCCCHHHHHHCCCCCCCCCCCCHHHH
SEVMTGMALGYTHFKFFPAEASGGVDALKAFSGPLADIRFCPTGGITPSSYKDYLALKNV
HHHHHHHHHHHHHHEEECCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHCCC
DCIGGSWIAPTDAMEHGDWDRITQLCKDAIGAL
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]