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The map label for this gene is puuA [H]

Identifier: 126174449

GI number: 126174449

Start: 2580493

End: 2581827

Strand: Reverse

Name: puuA [H]

Synonym: Sbal_2233

Alternate gene names: 126174449

Gene position: 2581827-2580493 (Counterclockwise)

Preceding gene: 126174450

Following gene: 126174442

Centisome position: 50.35

GC content: 48.24

Gene sequence:

>1335_bases
ATGGACTCAACCCTTCTTACTGCGTTTTTCCAGCACGGCACAATACGTGATGTCGAATGTTTATTCCCTGATATCTCAGG
CTATCCAAGGGGGAAGCTCATGCCAGCCCGAAGCTTTGCTAAGGGTGCTGAGTTACGTATTGCCCAAGCGATTTCGATGC
AGGCGATTACCGGTGAGTATTCCTACGATCCTATTTTCCCCGATGCTGATCCTGATCTGTTACTCGTCCCCGATTACAGC
ACCCTAAAAATGATCCCGTGGAGCACAGTGCCGCGCGCATTTGCCATCCATGACTGTGTGTTGCTCGATGGCAATTTATG
CCCATTTGCGCCGCGTTCTATTTTAAAAAATGTGTTGGCCCGTTACCAAGCCCTTGGGTTAACGCCCGTGGTGGCCCCTG
AGATTGAGTTTTATCTGACTGAAGCCAATACCGATCCGAGTGCGCCGTATCAGGCGCCCATTTCAAAAAGTGGCCGAGCT
GAAAACGGTCAATCTGCGTTTAGCATGAATATGCTCAATGAACTCGCGCCATTTTGGGATGAGTTTAATGCCGCGATCGA
TGCGATTGGGATCAAAGGTGATACTTGGATCCATGAGATGGGGCTATCGCAATATGAAATTAACTTAGTGCATGGCGATC
CGTTGGCCTTAGCAGATCAAGCTTTTATGTTTAAATACGCGGCGAAAGAAATCGCCATTAAACATGGCTTGAACGCGATT
TTTATGGCGAAACCCATAGCAGGCCAACCCGGCAGTTCTATGCATTTACATCAAAGTGTCGTGGATGATAAAGGCAAGAA
TATTTTCAGCCATGCAGATGGTAGCGATACTGAACATTTTCACCATTACATCGGCGGATTACAAAAGTATCTACCTGATT
TAATGTTGTTATTCGCACCCAATATCAATTCATTCCGACGTTACATTTCCGGTAGCCAAGCGCCGATTAATTTAAGCTGG
GGCGTAGATAACCGCACTACAGGCTTACGAGTGCCATTGAGTGCTCCGGTGGCGCGACGGGTAGAGAATCGCATTGCGGG
CGCCGATGCCAATCCGTATTTGATGATTGCCGCATCTTTGGCTGCGGGACTTGCGGGAATGGAAGAAAAACTATCCCCAA
GCGCGCCAGAAACCGGTAGTGCTTATGAGAGCAGCCATGACTTAGCGCAAACATTTCTCGCTGCATTAACGCAAATGCGC
CAAAGTGATTCGGCAAGGCGCTTACTTGGGGATGATTTTGTCACGGGTTTTGTGTCGGTGAAGGAGATTGAGTTTCAAAG
TTATTTGAGTGAGATAGGTGCTTGGGAGCGACGTTTCTTAGGGCCGCAATGCTAG

Upstream 100 bases:

>100_bases
TACAAATTTCATCGTCGCCATGTAGCTTTTCAAATCAAAGCCTGATTATACTTTGCATCCTTCATGGTGTTATTTTCAAC
ATTAGTGGCAAACGAATCAA

Downstream 100 bases:

>100_bases
CGCTTAATTGCTGAGGGGATGTTGCTGGATAACGCTTTATCCCTTGAGTGACTCGACATCCCACAAATCACGCGACAAAA
AAACTGCGACAAAAAAGCCA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAKGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVPDYS
TLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSAPYQAPISKSGRA
ENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGLNAI
FMAKPIAGQPGSSMHLHQSVVDDKGKNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPINLSW
GVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMR
QSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC

Sequences:

>Translated_444_residues
MDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAKGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVPDYS
TLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSAPYQAPISKSGRA
ENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGLNAI
FMAKPIAGQPGSSMHLHQSVVDDKGKNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPINLSW
GVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMR
QSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC
>Mature_444_residues
MDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAKGAELRIAQAISMQAITGEYSYDPIFPDADPDLLLVPDYS
TLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLARYQALGLTPVVAPEIEFYLTEANTDPSAPYQAPISKSGRA
ENGQSAFSMNMLNELAPFWDEFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGLNAI
FMAKPIAGQPGSSMHLHQSVVDDKGKNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAPNINSFRRYISGSQAPINLSW
GVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASLAAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMR
QSDSARRLLGDDFVTGFVSVKEIEFQSYLSEIGAWERRFLGPQC

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=24.1534988713318, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI87081870, Length=427, Percent_Identity=31.8501170960187, Blast_Score=198, Evalue=6e-52,
Organism=Escherichia coli, GI1790301, Length=331, Percent_Identity=29.3051359516616, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 48669; Mature: 48669

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAKGAELRIAQAISMQAITGEY
CCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
SYDPIFPDADPDLLLVPDYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLA
CCCCCCCCCCCCEEEECCCCCEEECCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHHHHH
RYQALGLTPVVAPEIEFYLTEANTDPSAPYQAPISKSGRAENGQSAFSMNMLNELAPFWD
HHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGLNAI
HHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEE
FMAKPIAGQPGSSMHLHQSVVDDKGKNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAP
EEECCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NINSFRRYISGSQAPINLSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASL
CHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEHHHH
AAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMRQSDSARRLLGDDFVTGFVSV
HHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KEIEFQSYLSEIGAWERRFLGPQC
HHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDSTLLTAFFQHGTIRDVECLFPDISGYPRGKLMPARSFAKGAELRIAQAISMQAITGEY
CCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
SYDPIFPDADPDLLLVPDYSTLKMIPWSTVPRAFAIHDCVLLDGNLCPFAPRSILKNVLA
CCCCCCCCCCCCEEEECCCCCEEECCCCCCCCHHHHEEEEEECCCCCCCCHHHHHHHHHH
RYQALGLTPVVAPEIEFYLTEANTDPSAPYQAPISKSGRAENGQSAFSMNMLNELAPFWD
HHHHCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EFNAAIDAIGIKGDTWIHEMGLSQYEINLVHGDPLALADQAFMFKYAAKEIAIKHGLNAI
HHHHHHHHCCCCCCCHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEE
FMAKPIAGQPGSSMHLHQSVVDDKGKNIFSHADGSDTEHFHHYIGGLQKYLPDLMLLFAP
EEECCCCCCCCCCCHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
NINSFRRYISGSQAPINLSWGVDNRTTGLRVPLSAPVARRVENRIAGADANPYLMIAASL
CHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCEEEHHHH
AAGLAGMEEKLSPSAPETGSAYESSHDLAQTFLAALTQMRQSDSARRLLGDDFVTGFVSV
HHHHHCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KEIEFQSYLSEIGAWERRFLGPQC
HHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]