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The map label for this gene is lcrF [H]

Identifier: 126174182

GI number: 126174182

Start: 2273386

End: 2274084

Strand: Direct

Name: lcrF [H]

Synonym: Sbal_1958

Alternate gene names: 126174182

Gene position: 2273386-2274084 (Clockwise)

Preceding gene: 126174181

Following gene: 126174183

Centisome position: 44.34

GC content: 42.2

Gene sequence:

>699_bases
ATGAGTGCAATACAAATAATTTTGTTTCGACAGCCAGGGAAAATCCACTGTCAGGGTGAAAACTTAGCGATAGACAGCAA
TCAGTTAGTCGTTACCACTGAGGAGGCTACAGCCTCGGCCATGAGTCGTGACAGTTGGTTGAGCATCTTTTGTTATCCAG
GGGAGTTACACGCCTTATATCACGAAATTGCCGATCTGATTGGCCCGAGCACTGAGATGAATCGATTTCAACTTGAATCA
GTAAAAATACTCCCCGTATTTGATGAGCTAAGCAATGTCATTGAACAGTTGAAAGGTAATCATAATTTATTGATTAAGTT
TATTTTTATGTACTGCCTGGGTATGGATAACGGTTACTTTTCCGGCTTAATGCGTTACTTCTTAGCCCATAACGACAAGA
TGCTGAGCTATCTCGAGGGCAATTTTATGCAACCTTGGCCAGTCACACGCTTTGCCGAAGATTTAGGCATTGAAGTACGC
CGACTCAATTTCTTGTTTTATAAAAACTATGGTGTATCGGCAAAGCAATGGTTGTTAGAACGGCGCTTAAGTTATGCCCG
TCAACAACTGATGGTGACCGAGAAGAAAGTCGCCGACATTGCCTTAGATAGTGGTTTTAGCAATCACGCACATTTTACCG
AATCGTTTAAGAAACGTTATTTATGCAGCCCAACCGTACTGCGTGCAAACTTCGCATAA

Upstream 100 bases:

>100_bases
GCTGCATTTAAGCGAATTCAGATCTCAGGTTAACACCTGATGTCAACCCATCCGCTCGCGTTTCACAATGAATCATAGTT
TGCAGTAAGAGTTAATCCAT

Downstream 100 bases:

>100_bases
GGAGGTCCCATGGACTTAAGTGCCATAGTCAATCAATTGTCTCAGCTATCGACGCGCTCGGCTCAAGAGGTACAGGCCAA
GATGTCTGCAGGTGATTTGA

Product: helix-turn-helix domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MSAIQIILFRQPGKIHCQGENLAIDSNQLVVTTEEATASAMSRDSWLSIFCYPGELHALYHEIADLIGPSTEMNRFQLES
VKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNGYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAEDLGIEVR
RLNFLFYKNYGVSAKQWLLERRLSYARQQLMVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRANFA

Sequences:

>Translated_232_residues
MSAIQIILFRQPGKIHCQGENLAIDSNQLVVTTEEATASAMSRDSWLSIFCYPGELHALYHEIADLIGPSTEMNRFQLES
VKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNGYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAEDLGIEVR
RLNFLFYKNYGVSAKQWLLERRLSYARQQLMVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRANFA
>Mature_231_residues
SAIQIILFRQPGKIHCQGENLAIDSNQLVVTTEEATASAMSRDSWLSIFCYPGELHALYHEIADLIGPSTEMNRFQLESV
KILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNGYFSGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAEDLGIEVRR
LNFLFYKNYGVSAKQWLLERRLSYARQQLMVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRANFA

Specific function: Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a te

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR014710 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 26772; Mature: 26641

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIQIILFRQPGKIHCQGENLAIDSNQLVVTTEEATASAMSRDSWLSIFCYPGELHALY
CCCEEEEEEECCCEEEECCCCEEECCCEEEEEECHHHHHHHCCCCEEEEEEECCHHHHHH
HEIADLIGPSTEMNRFQLESVKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNGYF
HHHHHHHCCCCCCCCEEECEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
SGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAEDLGIEVRRLNFLFYKNYGVSAKQWLLE
HHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHH
RRLSYARQQLMVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRANFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCC
>Mature Secondary Structure 
SAIQIILFRQPGKIHCQGENLAIDSNQLVVTTEEATASAMSRDSWLSIFCYPGELHALY
CCEEEEEEECCCEEEECCCCEEECCCEEEEEECHHHHHHHCCCCEEEEEEECCHHHHHH
HEIADLIGPSTEMNRFQLESVKILPVFDELSNVIEQLKGNHNLLIKFIFMYCLGMDNGYF
HHHHHHHCCCCCCCCEEECEEEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHH
SGLMRYFLAHNDKMLSYLEGNFMQPWPVTRFAEDLGIEVRRLNFLFYKNYGVSAKQWLLE
HHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEEEEEEECCCCCHHHHHHH
RRLSYARQQLMVTEKKVADIALDSGFSNHAHFTESFKKRYLCSPTVLRANFA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1624422; 9746557; 9748454; 11586360 [H]