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The map label for this gene is ligA [H]

Identifier: 126174078

GI number: 126174078

Start: 2151532

End: 2152479

Strand: Direct

Name: ligA [H]

Synonym: Sbal_1852

Alternate gene names: 126174078

Gene position: 2151532-2152479 (Clockwise)

Preceding gene: 126174076

Following gene: 126174080

Centisome position: 41.96

GC content: 45.57

Gene sequence:

>948_bases
ATGACTCATATCAGCCCGTTACAAGATTTAACATTTCATCGTTTTATCCGCTTTCGGTTTTTCAAACAACGTCTGCTCTT
ACTCAGTCTGCTAATATTCGGTTGTTTAGCCCCAGTGACCTATGCACAAACCTCACCCCCTTCGATACAGTTGGCAACCG
AGTTTGATGCCCAGCAAGAGATCCATATTCAGGACTTTTTGATTAGCGAAAAACTCGACGGGGTGCGAGGTTACTGGAAT
GGTCAGGCACTATTGACTCGGCAAGGTAATCTTATTGCTGCTCCAACTTGGTTTATCGCAAATTTTCCGAATTATCCCCT
CGATGGTGAATTATGGCTTGGGCGAGGCCAGTTTGAGGTTATGTCGAGCATAGTTCGACAAACCTCAGCCAAGGATGATG
ACTGGCGCAAAGTGCGATTTATGGTGTTCGATGTCCCTAAGGCGGGTGGCGACTTTCAGCATCGCTATCAATTTGCGTTG
ACCGAATTAAGCGATAAATCTCATTATCTTGAAGTCATAGCGCAGTTCCAAGTGGACAGTCTTGAAGCTCTGTATCAAAA
ACTTGATAGCTTAGTTGCTAAAGGCGCTGAAGGATTAATGCTGCATCGCAGCTCAGCGACGTATGTCAGTGGCCGCAATC
CTAATTTGATGAAATTAAAACCCTATTATGATGCTGAAGCGACAGTGATCGGCCATATACCAGGGAAAGGTCAATTTGCA
GGGCAAATGGGTGCAATAAGAGTGCAAACCCCAGACGGCCGGATTTTCAACATAGGCACAGGTTTTAATCTCGCAGAGCA
CCAACATCCTCCCGCAGTGGGCGCAATCATCACCTATAAATATCTGGGTCTTACCGTAAATGGCTTACCCAGATTTGCCA
GTTTCCTGCGGGTACGCGTCGATTTAGATGAGAGTAATAAAGTCATTTCATCGCAAGAAGGAAACTAG

Upstream 100 bases:

>100_bases
GTTAACTTAGGCGCTTAGTGACTTTCGACGGTTTTTTAGTGCAACTTGAGCTATGCGTTCTAAGCTTGATGAAGGATATA
TTGTCAGAGTGAGCAAGGGA

Downstream 100 bases:

>100_bases
CGGGCAGTTCTGCCCGCAATACTGCTCCGCTAGTATTTAAATTATTACTCGTAAAAAGTGACTGTAGTAAGTAGTTGTTC
ACTATAACTAAAAATATCGT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTHISPLQDLTFHRFIRFRFFKQRLLLLSLLIFGCLAPVTYAQTSPPSIQLATEFDAQQEIHIQDFLISEKLDGVRGYWN
GQALLTRQGNLIAAPTWFIANFPNYPLDGELWLGRGQFEVMSSIVRQTSAKDDDWRKVRFMVFDVPKAGGDFQHRYQFAL
TELSDKSHYLEVIAQFQVDSLEALYQKLDSLVAKGAEGLMLHRSSATYVSGRNPNLMKLKPYYDAEATVIGHIPGKGQFA
GQMGAIRVQTPDGRIFNIGTGFNLAEHQHPPAVGAIITYKYLGLTVNGLPRFASFLRVRVDLDESNKVISSQEGN

Sequences:

>Translated_315_residues
MTHISPLQDLTFHRFIRFRFFKQRLLLLSLLIFGCLAPVTYAQTSPPSIQLATEFDAQQEIHIQDFLISEKLDGVRGYWN
GQALLTRQGNLIAAPTWFIANFPNYPLDGELWLGRGQFEVMSSIVRQTSAKDDDWRKVRFMVFDVPKAGGDFQHRYQFAL
TELSDKSHYLEVIAQFQVDSLEALYQKLDSLVAKGAEGLMLHRSSATYVSGRNPNLMKLKPYYDAEATVIGHIPGKGQFA
GQMGAIRVQTPDGRIFNIGTGFNLAEHQHPPAVGAIITYKYLGLTVNGLPRFASFLRVRVDLDESNKVISSQEGN
>Mature_314_residues
THISPLQDLTFHRFIRFRFFKQRLLLLSLLIFGCLAPVTYAQTSPPSIQLATEFDAQQEIHIQDFLISEKLDGVRGYWNG
QALLTRQGNLIAAPTWFIANFPNYPLDGELWLGRGQFEVMSSIVRQTSAKDDDWRKVRFMVFDVPKAGGDFQHRYQFALT
ELSDKSHYLEVIAQFQVDSLEALYQKLDSLVAKGAEGLMLHRSSATYVSGRNPNLMKLKPYYDAEATVIGHIPGKGQFAG
QMGAIRVQTPDGRIFNIGTGFNLAEHQHPPAVGAIITYKYLGLTVNGLPRFASFLRVRVDLDESNKVISSQEGN

Specific function: Catalyzes efficient strand joining on a single nicked DNA [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF01068 DNA_ligase_A_M [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 35487; Mature: 35356

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHISPLQDLTFHRFIRFRFFKQRLLLLSLLIFGCLAPVTYAQTSPPSIQLATEFDAQQE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCC
IHIQDFLISEKLDGVRGYWNGQALLTRQGNLIAAPTWFIANFPNYPLDGELWLGRGQFEV
CHHHHHHHHHHCCCCCCEECCCEEEEECCCEEECCEEEEECCCCCCCCCCEEECCCHHHH
MSSIVRQTSAKDDDWRKVRFMVFDVPKAGGDFQHRYQFALTELSDKSHYLEVIAQFQVDS
HHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHH
LEALYQKLDSLVAKGAEGLMLHRSSATYVSGRNPNLMKLKPYYDAEATVIGHIPGKGQFA
HHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCEEEECCCCCCCEEEEEECCCCCCCC
GQMGAIRVQTPDGRIFNIGTGFNLAEHQHPPAVGAIITYKYLGLTVNGLPRFASFLRVRV
CCCCEEEEECCCCCEEEECCCCCCHHCCCCCCHHHEEEEEEEEEEECCHHHHHHEEEEEE
DLDESNKVISSQEGN
EECCCCCCCCCCCCC
>Mature Secondary Structure 
THISPLQDLTFHRFIRFRFFKQRLLLLSLLIFGCLAPVTYAQTSPPSIQLATEFDAQQE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCC
IHIQDFLISEKLDGVRGYWNGQALLTRQGNLIAAPTWFIANFPNYPLDGELWLGRGQFEV
CHHHHHHHHHHCCCCCCEECCCEEEEECCCEEECCEEEEECCCCCCCCCCEEECCCHHHH
MSSIVRQTSAKDDDWRKVRFMVFDVPKAGGDFQHRYQFALTELSDKSHYLEVIAQFQVDS
HHHHHHHHCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCHHH
LEALYQKLDSLVAKGAEGLMLHRSSATYVSGRNPNLMKLKPYYDAEATVIGHIPGKGQFA
HHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCEEEECCCCCCCEEEEEECCCCCCCC
GQMGAIRVQTPDGRIFNIGTGFNLAEHQHPPAVGAIITYKYLGLTVNGLPRFASFLRVRV
CCCCEEEEECCCCCEEEECCCCCCHHCCCCCCHHHEEEEEEEEEEECCHHHHHHEEEEEE
DLDESNKVISSQEGN
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550458; 7542800; 9060431 [H]