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The map label for this gene is sbcD [H]

Identifier: 126173886

GI number: 126173886

Start: 1931302

End: 1932447

Strand: Direct

Name: sbcD [H]

Synonym: Sbal_1654

Alternate gene names: 126173886

Gene position: 1931302-1932447 (Clockwise)

Preceding gene: 126173885

Following gene: 126173887

Centisome position: 37.67

GC content: 48.69

Gene sequence:

>1146_bases
ATGAAATTTATCCACACCTCGGACTGGCACATAGGTCGTCAGTTACACAATCAATCATTACTGGATGATCAAGCCTTTGT
GCTCGAGCAAATTATTGCATTAGCAGATGAGCATAAGGTGGATGCTGTGATTGTGGCGGGGGATATTTATGACAGATCGA
TTCCGCCAGCAAACGCGGTTGCGCTGTTAGATGATGTGCTCAATCGCTTAGTACAAGATTTAGGCCTGCAAGTAATTATG
ATTGCCGGTAACCACGATGGCCACGAGCGCTTAGGGTTTGCGGCAAAACAAATGGCGACAAGCGGGTTACATATTATTGG
GCCTTTGAGTGCTGACATTCATCCTATTACTTTGTCGAGTTCAAGCGGTGATGTGGTTTTTTATCCCTTGCCTTACGCTG
AACCTGCAACAGTTCGCCAAGTGTTTGAGTGTGAGGCATCGAGCCACGAAATGGCCATGGATGTGTTGCTGGAAAAAGTG
CGCCAGCACGATAGCCAAGGCTTACCCAAAGTGGTGATCAGCCATTGCTTTTTGGATGGTGGCAGTGAGTCAGAATCAGA
GCGGCCGCTCAGTATTGGTGGCGCCGATAAAATATCGCCAAAGCTCTTTACTGAGTTTGATTATGTCGCCCTTGGGCATT
TGCACGGACCGCAATACAAAGGCGCCGAGCATGTGCGCTACTCAGGATCGATTTTGAAGTATTCCTTTAGTGAACAACAT
CAACACAAGTCGGTGACCTTAGTCGAACTGGGCACGCAAACGCCGATTGATATCCGTTTATTGCCGTTAACGTCGATGCG
TGATGTTCGCATCTTAGAAGGGGAGTTAGACACTTTACTCGCCCAAGGTAAAACGGATGCAAACCGTGAGGATTACCTTA
TGGTTAGGCTCCTCGATAAACATGCCATTTTAGACGCTATGGGCAAGCTTAGGGCCGTCTACCCTAATGTGCTGCATTTA
GAGCGGACAGGCCTGATGGCGGGGCAGCAACAGGTCGCTTTGAGCCGGGATCATATCAAAAAAGGCGAGATGGACATGTT
CTGTGACTTTTTCTCTCAGGTGTCTGGCGAGTCATTGACGCAGGCACAGCAAGCCGTGATGGATGACCTCCTGACTAAGC
TACACAATGAGGAGCGCCAAGCATGA

Upstream 100 bases:

>100_bases
TTGCATGCGTGCAGTGTCTAGCTCGTCTGCCTTATTTTTGCGTTGATGTGCCGGCACATGCGCTCGGGTTTAGCGCTACC
ACTTCTTTAAAAGAGCGATT

Downstream 100 bases:

>100_bases
GACCCCTTAGCCTTTCAATGTCAGCCTTCGGCCCCTTTGCATCGACTCAGACACTTGATTTTACCGCCCTTGGAACCAAT
CCATTATTTTTAATCAACGG

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKFIHTSDWHIGRQLHNQSLLDDQAFVLEQIIALADEHKVDAVIVAGDIYDRSIPPANAVALLDDVLNRLVQDLGLQVIM
IAGNHDGHERLGFAAKQMATSGLHIIGPLSADIHPITLSSSSGDVVFYPLPYAEPATVRQVFECEASSHEMAMDVLLEKV
RQHDSQGLPKVVISHCFLDGGSESESERPLSIGGADKISPKLFTEFDYVALGHLHGPQYKGAEHVRYSGSILKYSFSEQH
QHKSVTLVELGTQTPIDIRLLPLTSMRDVRILEGELDTLLAQGKTDANREDYLMVRLLDKHAILDAMGKLRAVYPNVLHL
ERTGLMAGQQQVALSRDHIKKGEMDMFCDFFSQVSGESLTQAQQAVMDDLLTKLHNEERQA

Sequences:

>Translated_381_residues
MKFIHTSDWHIGRQLHNQSLLDDQAFVLEQIIALADEHKVDAVIVAGDIYDRSIPPANAVALLDDVLNRLVQDLGLQVIM
IAGNHDGHERLGFAAKQMATSGLHIIGPLSADIHPITLSSSSGDVVFYPLPYAEPATVRQVFECEASSHEMAMDVLLEKV
RQHDSQGLPKVVISHCFLDGGSESESERPLSIGGADKISPKLFTEFDYVALGHLHGPQYKGAEHVRYSGSILKYSFSEQH
QHKSVTLVELGTQTPIDIRLLPLTSMRDVRILEGELDTLLAQGKTDANREDYLMVRLLDKHAILDAMGKLRAVYPNVLHL
ERTGLMAGQQQVALSRDHIKKGEMDMFCDFFSQVSGESLTQAQQAVMDDLLTKLHNEERQA
>Mature_381_residues
MKFIHTSDWHIGRQLHNQSLLDDQAFVLEQIIALADEHKVDAVIVAGDIYDRSIPPANAVALLDDVLNRLVQDLGLQVIM
IAGNHDGHERLGFAAKQMATSGLHIIGPLSADIHPITLSSSSGDVVFYPLPYAEPATVRQVFECEASSHEMAMDVLLEKV
RQHDSQGLPKVVISHCFLDGGSESESERPLSIGGADKISPKLFTEFDYVALGHLHGPQYKGAEHVRYSGSILKYSFSEQH
QHKSVTLVELGTQTPIDIRLLPLTSMRDVRILEGELDTLLAQGKTDANREDYLMVRLLDKHAILDAMGKLRAVYPNVLHL
ERTGLMAGQQQVALSRDHIKKGEMDMFCDFFSQVSGESLTQAQQAVMDDLLTKLHNEERQA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=401, Percent_Identity=27.930174563591, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42254; Mature: 42254

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIHTSDWHIGRQLHNQSLLDDQAFVLEQIIALADEHKVDAVIVAGDIYDRSIPPANAV
CCCEECCCCHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
ALLDDVLNRLVQDLGLQVIMIAGNHDGHERLGFAAKQMATSGLHIIGPLSADIHPITLSS
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEC
SSGDVVFYPLPYAEPATVRQVFECEASSHEMAMDVLLEKVRQHDSQGLPKVVISHCFLDG
CCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GSESESERPLSIGGADKISPKLFTEFDYVALGHLHGPQYKGAEHVRYSGSILKYSFSEQH
CCCCCCCCCEECCCCCCCCCHHHCCCCCEEEECCCCCCCCCCCCEEECCCEEEEECHHHH
QHKSVTLVELGTQTPIDIRLLPLTSMRDVRILEGELDTLLAQGKTDANREDYLMVRLLDK
CCCCEEEEEECCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEHHHH
HAILDAMGKLRAVYPNVLHLERTGLMAGQQQVALSRDHIKKGEMDMFCDFFSQVSGESLT
HHHHHHHHHHHHHCCCCEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
QAQQAVMDDLLTKLHNEERQA
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFIHTSDWHIGRQLHNQSLLDDQAFVLEQIIALADEHKVDAVIVAGDIYDRSIPPANAV
CCCEECCCCHHHHHHHCCHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHH
ALLDDVLNRLVQDLGLQVIMIAGNHDGHERLGFAAKQMATSGLHIIGPLSADIHPITLSS
HHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEC
SSGDVVFYPLPYAEPATVRQVFECEASSHEMAMDVLLEKVRQHDSQGLPKVVISHCFLDG
CCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GSESESERPLSIGGADKISPKLFTEFDYVALGHLHGPQYKGAEHVRYSGSILKYSFSEQH
CCCCCCCCCEECCCCCCCCCHHHCCCCCEEEECCCCCCCCCCCCEEECCCEEEEECHHHH
QHKSVTLVELGTQTPIDIRLLPLTSMRDVRILEGELDTLLAQGKTDANREDYLMVRLLDK
CCCCEEEEEECCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCCCCCCCCCCEEEEEHHHH
HAILDAMGKLRAVYPNVLHLERTGLMAGQQQVALSRDHIKKGEMDMFCDFFSQVSGESLT
HHHHHHHHHHHHHCCCCEEEEHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHH
QAQQAVMDDLLTKLHNEERQA
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA