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The map label for this gene is spsK [H]

Identifier: 126173711

GI number: 126173711

Start: 1713175

End: 1714320

Strand: Direct

Name: spsK [H]

Synonym: Sbal_1473

Alternate gene names: 126173711

Gene position: 1713175-1714320 (Clockwise)

Preceding gene: 126173710

Following gene: 126173712

Centisome position: 33.41

GC content: 49.13

Gene sequence:

>1146_bases
ATGAAAATAGTGGCAATTAAAGTGACGCCACTTCAATGTGTTGAGACAAGAGCCACAGACGCTAACACTCCTCAAGTGGT
AATAATCACGGGCGCGAAGAGTCAGCTTGCTCAGGCGTTACTGAGAGTCGCTGCCGATATTGGCGAGCTAGCCCTAAATA
GCATCACAAGTGAAAACACAGGCGCACCGCTGAAGCTTTATGCGTTAAGTCGCAGTCAGTTAGATATTACCGATGCCGCC
AATATTGCAGCAGTGTTTGATCAATATCGACCAAGCTGGGTGATCAATTGCGCGGCCTATAATGCGGTAGACGCTGCTGA
GCATGACGCAATCGAAGCGCATCGGGTGAATGCACTCGGCCCTGAGTTATTGGCGCAGCAATGTTTGCAGTCGGGCGCGC
GCTTACTGCATGTGTCGAGCGATTATGTGTTTGGTGGTCATACTGTTTGCGGTATTGCTCATGCAGCTGAACGTGCTGTT
TGCGATGCTAGAGAAAGTGGCGTTGAACAACATCAAAACCCAGACTTAGCTCCAAATTCAAATCCAAACCATTTACCAAG
GCCATTTGTTGAACTCGATGCTCCCGAGCCGTTGTCTACTTATGGTAAGAGTAAGTTGCTCGGCGAGCTGAAGGTAGTGG
CCATCTTGGGGGAGCGCGCAACGATTGTCCGCACCTCATGGCTCTACGGTCAAAATGGGCATAATTTTGTCAAAACCATG
CTGAACTTGATGCGTACTCAGCCAAGTTTGCAGGTAATAGCCGATCAAATCGGTTGCCCGACATGGAGTGATGCACTCGC
GCAGGTGATATGGCAATTAGTCGTGCAGCAGTGCTCGGGCGTATTTCATTACAGCGCTCAAGGTCAGTGCAGTTGGTATG
AGTTTGCCTGTGAAATCCAGCGACAAGCCCTAGCGCTCAATCTGTTAAGTCTACCTGTGGTTATCCAGCCAATTACCAGC
GCTCATTATGCTAAACAAGCACTAAATCGCGGCGTGGGTTTGGCAAAAAGGCCGAGTTATAGTGTGTTGAACAGTGGTAA
ATTACGCTCCACTTTGGTGACCAATAACACACAGTCGATACCTGATAACGTGGAGTGGCAAGATTGGCGTCAGCAACTGA
ATTGCATGTTGCGGCGGTTATCTTGA

Upstream 100 bases:

>100_bases
TCCTTCTGCACTCTTGGCCTTTTTAGCCGCCCGTGGTCATGAAACCGTCGCCGATTTGATCCCCTTATTTAGCGACGATG
AGCCATGCTGTTGATCCGTG

Downstream 100 bases:

>100_bases
CGATAATGAGCGTGACTCACATTTTGCTTTAGGTTGCTTTAATCACGCTTTAAGCGTTTACGTCATCTAAGTGATTAACG
TTGTCGAAAACAATAAGCCC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKIVAIKVTPLQCVETRATDANTPQVVIITGAKSQLAQALLRVAADIGELALNSITSENTGAPLKLYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEAHRVNALGPELLAQQCLQSGARLLHVSSDYVFGGHTVCGIAHAAERAV
CDARESGVEQHQNPDLAPNSNPNHLPRPFVELDAPEPLSTYGKSKLLGELKVVAILGERATIVRTSWLYGQNGHNFVKTM
LNLMRTQPSLQVIADQIGCPTWSDALAQVIWQLVVQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSLPVVIQPITS
AHYAKQALNRGVGLAKRPSYSVLNSGKLRSTLVTNNTQSIPDNVEWQDWRQQLNCMLRRLS

Sequences:

>Translated_381_residues
MKIVAIKVTPLQCVETRATDANTPQVVIITGAKSQLAQALLRVAADIGELALNSITSENTGAPLKLYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEAHRVNALGPELLAQQCLQSGARLLHVSSDYVFGGHTVCGIAHAAERAV
CDARESGVEQHQNPDLAPNSNPNHLPRPFVELDAPEPLSTYGKSKLLGELKVVAILGERATIVRTSWLYGQNGHNFVKTM
LNLMRTQPSLQVIADQIGCPTWSDALAQVIWQLVVQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSLPVVIQPITS
AHYAKQALNRGVGLAKRPSYSVLNSGKLRSTLVTNNTQSIPDNVEWQDWRQQLNCMLRRLS
>Mature_381_residues
MKIVAIKVTPLQCVETRATDANTPQVVIITGAKSQLAQALLRVAADIGELALNSITSENTGAPLKLYALSRSQLDITDAA
NIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEAHRVNALGPELLAQQCLQSGARLLHVSSDYVFGGHTVCGIAHAAERAV
CDARESGVEQHQNPDLAPNSNPNHLPRPFVELDAPEPLSTYGKSKLLGELKVVAILGERATIVRTSWLYGQNGHNFVKTM
LNLMRTQPSLQVIADQIGCPTWSDALAQVIWQLVVQQCSGVFHYSAQGQCSWYEFACEIQRQALALNLLSLPVVIQPITS
AHYAKQALNRGVGLAKRPSYSVLNSGKLRSTLVTNNTQSIPDNVEWQDWRQQLNCMLRRLS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=367, Percent_Identity=28.3378746594005, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 41608; Mature: 41608

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVAIKVTPLQCVETRATDANTPQVVIITGAKSQLAQALLRVAADIGELALNSITSENT
CEEEEEEECCHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAPLKLYALSRSQLDITDAANIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEAHRVNALG
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCC
PELLAQQCLQSGARLLHVSSDYVFGGHTVCGIAHAAERAVCDARESGVEQHQNPDLAPNS
HHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NPNHLPRPFVELDAPEPLSTYGKSKLLGELKVVAILGERATIVRTSWLYGQNGHNFVKTM
CCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEEEECCCCHHHHHHH
LNLMRTQPSLQVIADQIGCPTWSDALAQVIWQLVVQQCSGVFHYSAQGQCSWYEFACEIQ
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHH
RQALALNLLSLPVVIQPITSAHYAKQALNRGVGLAKRPSYSVLNSGKLRSTLVTNNTQSI
HHHHHHHHHHCCHHEECHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCCCCC
PDNVEWQDWRQQLNCMLRRLS
CCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKIVAIKVTPLQCVETRATDANTPQVVIITGAKSQLAQALLRVAADIGELALNSITSENT
CEEEEEEECCHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAPLKLYALSRSQLDITDAANIAAVFDQYRPSWVINCAAYNAVDAAEHDAIEAHRVNALG
CCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCC
PELLAQQCLQSGARLLHVSSDYVFGGHTVCGIAHAAERAVCDARESGVEQHQNPDLAPNS
HHHHHHHHHHCCCEEEEECCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NPNHLPRPFVELDAPEPLSTYGKSKLLGELKVVAILGERATIVRTSWLYGQNGHNFVKTM
CCCCCCCCCEECCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEEEEEEECCCCHHHHHHH
LNLMRTQPSLQVIADQIGCPTWSDALAQVIWQLVVQQCSGVFHYSAQGQCSWYEFACEIQ
HHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHH
RQALALNLLSLPVVIQPITSAHYAKQALNRGVGLAKRPSYSVLNSGKLRSTLVTNNTQSI
HHHHHHHHHHCCHHEECHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEECCCCCC
PDNVEWQDWRQQLNCMLRRLS
CCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]