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The map label for this gene is anfA [H]

Identifier: 126173655

GI number: 126173655

Start: 1633486

End: 1634859

Strand: Direct

Name: anfA [H]

Synonym: Sbal_1417

Alternate gene names: 126173655

Gene position: 1633486-1634859 (Clockwise)

Preceding gene: 126173652

Following gene: 126173665

Centisome position: 31.86

GC content: 49.78

Gene sequence:

>1374_bases
ATGGCTCGATTGACACTTTTTTATCACCTGCAGGATGTAAACGCCGAACAAGCCATCTTAGAACTCGCCGGCGCTGCCGA
CTTTAATAAGCTACGCAGTGTCGATGATTTACCTTGGATAGAACAGCTTGAGGCGAATCACGTCGATGTTGCCATCATTG
AGCTGTCCAGCCTTAGCCGTGAGGATTATCTCGGGCTACTGGACAATGTAGCTCTGTCCAATATCGAGTTTATTTTTCTC
TCCGACGGCCAGCCGAACCCCAATCTTGATCAGTTAATGTCGCATTTTGCCGGTTATCATTTTCGAAAACCCTACGACAT
GACGTTAATCAGCGATATTTTCGCCGATTTTGCTCAGTATCTAGTGTCATCCCCAATTCGGCCTCGGCCTTTTTCCAGTG
AGCTAGACCAATATGGTTTACTCGTCGGTTCCTCTGCTGCGATGCATAAGCTGTATCGAACGATTCGCCGCGTCTCCGCG
ACCGACAGTAATGTGCTGATCATCGGTGAGAGCGGTGCGGGGAAAGAGCTGGTCGCTAATACCATACATCTTGCCAGCCC
TAGGCTGAACGAGCCTTTTATCGCGATTAACTGCGGCGCCTTAAGCCCTGAATTGGTCGATAGCGAGTTGTTTGGCCACG
TAAAAGGCGCATTCACTGGCGCTCATCGCGATCACAAAGGCGTATTTGAGCAGGCAGAAGGCGGAACCTTATTTCTCGAT
GAAGTCACCGAAATGCCAATTGAGCATCAAGTGAAGTTACTCAGAGTGCTTGAAAATAGCGAATATCGCCCCGTTGGCGG
TGCCAAAGTGCAACTCGCCAATGTGCGGATTGTCGCGGCCACCAATCGTGATCCCATCGCGGCAATCGAGGCTGGGCAAT
TTCGCGAGGATTTATATTTCCGTTTGGCCCAGTTCCCTATCCGCGTTCCGCCATTAAGGGAACGTGGCGATGATATTCTT
GGGTTGGCGCAGCATTTTCTTGCCTATCGTAACGTGGCAGAAAAGCAGAATAAAAGCCTCGCTGACGACAGTGCGCAGAT
GATCGCTGCGTACCCTTGGTCGGGTAATGTGCGTGAATTAAAGCATGCCATTGAGCGAGCCTATATTTTGGCAGACCAAG
AGATTTTACCCAGCCATTTACAGTTGCCACAGGCAAGTGAAATGGGTGCTTCTGGCGATGACCAAGTCGTTATCCCGCAG
GGCATGCGTTTAGATGACTTAGAAAAAATCGCCATTTATCAAGCATTAGAAACGACTCAAGGCAATAAAACCGATACCGC
CGAGCAGCTGGGGATCAGCGTGAAAACTCTCTACAACAAGCTCAGCAAGTACGAGCAGCAAGTCGAAGCGGGTGAATCGA
ATGCAGAGATGTAG

Upstream 100 bases:

>100_bases
TACGCCATCCTCTTGGAATATCGGCAACTATTACCGCTGCTGTTGTTAAAATTTCACGACTGACATACAGTAGCCCTTCA
TACACAGGGAGTGGGATAGC

Downstream 100 bases:

>100_bases
TTTTTAGGCTTAAAAGTGAAGGTTATGGGGGCGTTTACTTAGATTGCGATGAATGCTTTATGGCCCTTAAGCAAAAATGA
GCTGCAACTTTGCAGTTGCA

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFL
SDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSA
TDSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDIL
GLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGASGDDQVVIPQ
GMRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESNAEM

Sequences:

>Translated_457_residues
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFL
SDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSA
TDSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDIL
GLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGASGDDQVVIPQ
GMRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESNAEM
>Mature_456_residues
ARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSREDYLGLLDNVALSNIEFIFLS
DGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQYLVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSAT
DSNVLIIGESGAGKELVANTIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLDE
VTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYFRLAQFPIRVPPLRERGDDILG
LAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVRELKHAIERAYILADQEILPSHLQLPQASEMGASGDDQVVIPQG
MRLDDLEKIAIYQALETTQGNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESNAEM

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=45.9807073954984, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788550, Length=315, Percent_Identity=42.2222222222222, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI87082117, Length=319, Percent_Identity=40.7523510971787, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=40.7407407407407, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1788905, Length=308, Percent_Identity=41.2337662337662, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082152, Length=313, Percent_Identity=42.1725239616613, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790299, Length=327, Percent_Identity=39.4495412844037, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=37.2670807453416, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI87081872, Length=317, Percent_Identity=37.8548895899054, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1786524, Length=324, Percent_Identity=37.962962962963, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1787583, Length=321, Percent_Identity=34.5794392523364, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=34.0807174887892, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=29.0849673202614, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50744; Mature: 50613

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSR
CCEEEEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCH
EDYLGLLDNVALSNIEFIFLSDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQY
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSATDSNVLIIGESGAGKELVAN
HHCCCCCCCCCHHHHHHHCEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEC
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYF
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHHH
RLAQFPIRVPPLRERGDDILGLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVREL
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
KHAIERAYILADQEILPSHLQLPQASEMGASGDDQVVIPQGMRLDDLEKIAIYQALETTQ
HHHHHHHHHEECHHHCHHHHCCCCHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCC
GNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESNAEM
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARLTLFYHLQDVNAEQAILELAGAADFNKLRSVDDLPWIEQLEANHVDVAIIELSSLSR
CEEEEEEEEECCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHCCCCCEEEEEEECCCCH
EDYLGLLDNVALSNIEFIFLSDGQPNPNLDQLMSHFAGYHFRKPYDMTLISDIFADFAQY
HHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LVSSPIRPRPFSSELDQYGLLVGSSAAMHKLYRTIRRVSATDSNVLIIGESGAGKELVAN
HHCCCCCCCCCHHHHHHHCEEECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHH
TIHLASPRLNEPFIAINCGALSPELVDSELFGHVKGAFTGAHRDHKGVFEQAEGGTLFLD
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEC
EVTEMPIEHQVKLLRVLENSEYRPVGGAKVQLANVRIVAATNRDPIAAIEAGQFREDLYF
HHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEECCHHHHHHHHH
RLAQFPIRVPPLRERGDDILGLAQHFLAYRNVAEKQNKSLADDSAQMIAAYPWSGNVREL
HHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHH
KHAIERAYILADQEILPSHLQLPQASEMGASGDDQVVIPQGMRLDDLEKIAIYQALETTQ
HHHHHHHHHEECHHHCHHHHCCCCHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCC
GNKTDTAEQLGISVKTLYNKLSKYEQQVEAGESNAEM
CCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]