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The map label for this gene is gcvA [H]

Identifier: 126173602

GI number: 126173602

Start: 1564239

End: 1565150

Strand: Direct

Name: gcvA [H]

Synonym: Sbal_1364

Alternate gene names: 126173602

Gene position: 1564239-1565150 (Clockwise)

Preceding gene: 126173600

Following gene: 126173603

Centisome position: 30.51

GC content: 48.9

Gene sequence:

>912_bases
ATGTCCAGACGATTACCACCGCTTAATGCGGTTAAAGCATTTGAGGCAGCCGCGCGGCATTTGAGCTTTACGCGCGCAGC
AGAAGAATTGTTTGTTACGCAGGCTGCGGTGAGCCATCAAATAAAGGCATTAGAAGATTTCCTCGGATTAAAATTATTTC
GCCGAAAGAACCGCTCTTTGCTTTTAACTGAAGAAGGGCAGAGCTACTTCCTCGACATCAAAGACATCTTTATTCAACTC
GCCGATGCTACCGACCGATTACTCGCCCGCAGCGCGATTGGCTCGCTGACGGTCAGTATGTCGCCCAGTTTTGCAATTCA
ATGGTTAGTGCCGCGTTTGGCAAAGTTCAGTGAGAAAAATCCCGATATCGACGTGCGTATTAAAGCCGTCGATACCGAAG
CGAGCTCATTAACCGATGATGTGGATGTCGCGATTTATTATGGTCTAGGCAATTGGCCTGGTCTGCGCGCCGATAAGCTC
AGAAACGAAGTGCTCATTCCTGTGTGTTCGCCCATGTTGCTCAACGGTCCTAAGCCGTTGTCAAAACCGAGCGATTTAAA
ATATCACACGCTATTACATGATATGAATCGTCACGATTGGCAGGCGTGGTTTAGACAATGTGGGATTAACGATATTAATG
TGAACCAAGGCCCCATTTTCAGTCATTCGTCCTTGGTATTGCAGGCGGCGGCCCATGGTCAAGGCGTGGCTTTAGGTTAC
AGCGTACTGGCGCGTCCCGATATAAAAGCGGGGCGTCTCGTGTGCCCATTCCAAGAAGTCTTGGTCAGTAAAGATGCTTA
CTACTTGGTTTGCCAGCAGAATCACGCAGAACTGGGTAAGGTCGTCGCGTTTCGCGAATGGATGTTAGATATGTTTGCTG
AGGAGTCGCGTAGTGAGTTGTTGCCCGGATAA

Upstream 100 bases:

>100_bases
CAATGGCGCAAAATTTAACCAAAATCACTGGCCTTTACAAACGATTAAATGTAACAATACGGATTAGAAAAACTAATTAA
AAAACGAGACTGAGATCACT

Downstream 100 bases:

>100_bases
GCCAAGTTTTGTATTTGAGGGTGAGCCTGCGAGCACAATGATTTTATTGGCCCACGGCGCGGGCGCTAACATGGACTCTG
AGTTTATGCAGGCCATGTCG

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFIQL
ADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKL
RNEVLIPVCSPMLLNGPKPLSKPSDLKYHTLLHDMNRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG

Sequences:

>Translated_303_residues
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFIQL
ADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKL
RNEVLIPVCSPMLLNGPKPLSKPSDLKYHTLLHDMNRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGY
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG
>Mature_302_residues
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSLLLTEEGQSYFLDIKDIFIQLA
DATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKNPDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLR
NEVLIPVCSPMLLNGPKPLSKPSDLKYHTLLHDMNRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGYS
VLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSELLPG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=70.8474576271186, Blast_Score=439, Evalue=1e-125,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=36.2369337979094, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788706, Length=296, Percent_Identity=30.7432432432432, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=26.2458471760797, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786401, Length=236, Percent_Identity=28.3898305084746, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI157672245, Length=185, Percent_Identity=30.8108108108108, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87081978, Length=298, Percent_Identity=28.8590604026846, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789639, Length=194, Percent_Identity=26.8041237113402, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787879, Length=128, Percent_Identity=32.8125, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787128, Length=135, Percent_Identity=29.6296296296296, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33903; Mature: 33772

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFIQLADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLRNEVLIPVCSPMLLNGPKPL
CCEEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
SKPSDLKYHTLLHDMNRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSEL
HHHCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPG
CCC
>Mature Secondary Structure 
SRRLPPLNAVKAFEAAARHLSFTRAAEELFVTQAAVSHQIKALEDFLGLKLFRRKNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
LLTEEGQSYFLDIKDIFIQLADATDRLLARSAIGSLTVSMSPSFAIQWLVPRLAKFSEKN
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCC
PDIDVRIKAVDTEASSLTDDVDVAIYYGLGNWPGLRADKLRNEVLIPVCSPMLLNGPKPL
CCEEEEEEEECCCHHHCCCCCEEEEEEECCCCCCCCHHHHCCCEEEEECCHHHCCCCCCC
SKPSDLKYHTLLHDMNRHDWQAWFRQCGINDINVNQGPIFSHSSLVLQAAAHGQGVALGY
CCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHEEECCCCCCEEEHH
SVLARPDIKAGRLVCPFQEVLVSKDAYYLVCQQNHAELGKVVAFREWMLDMFAEESRSEL
HHHCCCCCCCCCEECCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
LPG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]