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The map label for this gene is nemA [H]

Identifier: 126173487

GI number: 126173487

Start: 1435774

End: 1436841

Strand: Direct

Name: nemA [H]

Synonym: Sbal_1249

Alternate gene names: 126173487

Gene position: 1435774-1436841 (Clockwise)

Preceding gene: 126173485

Following gene: 126173489

Centisome position: 28.0

GC content: 48.78

Gene sequence:

>1068_bases
ATGACCACACAAACCACGCCAAATTCCTTGGCAAATCTGTTCGAAACCTACTCGCTTAATGACACTATTACCTTAAACAA
TCGTTTCTTAATGGCACCTTTGACCCGTTGTATGGCCGATGCCGACTTAGTGCCAACCGATGATATGGTTGCCTATTATG
CACGCCGCGCCGAAGCGGGTTTGATCATCACAGAGGCGACCATTATTCGTCCCGATGCCCAAGGTTATCCAAATACCCCA
GGTATTTTTACTCAAGCGCAAATTGCTGGCTGGCGCAAAGTGACCGATGCAGTACATGCCAATGGCGGTAAGATTTTTGT
GCAGTTATGGCATACGGGCCGTGTTGCTCATCCACACTTTTTCGGTGGTGGCGATGTACTTGCGGCATCAGCACAGAAGA
TTGAAGGCAGCGTGCCAAGAATGCGTGAGCTAACTTATGTCACGCCAAAACCAGCCACACTTGAGGATATTCAAGGCCTA
GTGCGTGATTACGCCAAAGCGGCGGAAAATGCCATTGAAGCTGGCTTTGATGGCGTTGAGATCCACGGTGCAAATGGTTA
TTTAATTGATACTTTCTTACACCATGACAGCAATCGCCGCACCGATGAATACGGTGGCACGCCAGCGAATATGTCCCGTT
TTGCGCTTGAAGTTGTTGACGCTGTTGTGGCTCGTATCGGCAAAGACAGAACTGGTCTACGGATTTCTCCAGGGGCTTAT
TTCAATATGGCGGCAGATAGCCGTGACCGCGAAGTGTTCGATTATTTCTTACCTGAGTTAGAGTCGCGTGATATCGCCTT
TGTGCATATTGGGATTTTCGATGACAGTATGGAATTCGACTATCTTGGCGGCAGAGCTTCAAGCTATGTGCGCGCACACT
ATGGCAAAACCTTAGTGGGAGTGGGAAGCTACAGCGCTGAAACCGCCAGTGAAGCGATTGCAGCGAATAAGTTCGATTTG
ATTGCTATCGGTCGTCCATTCATTGCTAACCCTGACTATGTTGCTCGCGTGCGTGATGGCGTTGAACTTGTGCCTTACAG
CGACGAAATGCTAGCAAGCTTAGTGTAA

Upstream 100 bases:

>100_bases
CCGAATTCTACCTAATTTTGAATGAACATTCAAAAATTAACTTGAATGATCGTTCAAATAATAATATCTTGAATGCATAT
TCAAGAATGGGACTGAGAAA

Downstream 100 bases:

>100_bases
TTTCACTTAATAGCTGAATAAAGAAAGGGTTAGCATTGGCTAACCCTTTTTGTGTGTCTTTTTCGCGTTTTTATTTAATC
CGCTTTAACTAAGGATCTAA

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTDDMVAYYARRAEAGLIITEATIIRPDAQGYPNTP
GIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDL
IAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV

Sequences:

>Translated_355_residues
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTDDMVAYYARRAEAGLIITEATIIRPDAQGYPNTP
GIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGL
VRDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDL
IAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
>Mature_354_residues
TTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTDDMVAYYARRAEAGLIITEATIIRPDAQGYPNTPG
IFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHFFGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLV
RDYAKAAENAIEAGFDGVEIHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAYF
NMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVGVGSYSAETASEAIAANKFDLI
AIGRPFIANPDYVARVRDGVELVPYSDEMLASLV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=341, Percent_Identity=41.0557184750733, Blast_Score=241, Evalue=5e-65,
Organism=Escherichia coli, GI1789463, Length=347, Percent_Identity=28.5302593659942, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=359, Percent_Identity=27.5766016713092, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=350, Percent_Identity=27.4285714285714, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=342, Percent_Identity=28.3625730994152, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564188, Length=350, Percent_Identity=27.4285714285714, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=365, Percent_Identity=27.3972602739726, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=363, Percent_Identity=25.3443526170799, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=364, Percent_Identity=37.3626373626374, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6321973, Length=358, Percent_Identity=36.8715083798883, Blast_Score=189, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38890; Mature: 38759

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCCCCCHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIITEATIIRPDAQGYPNTPGIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEEHHHHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAANKFDLIAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHCC
>Mature Secondary Structure 
TTQTTPNSLANLFETYSLNDTITLNNRFLMAPLTRCMADADLVPTDDMVAYYARRAEAG
CCCCCHHHHHHHHHHCCCCCEEEECCEEEHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
LIITEATIIRPDAQGYPNTPGIFTQAQIAGWRKVTDAVHANGGKIFVQLWHTGRVAHPHF
EEEEEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEE
FGGGDVLAASAQKIEGSVPRMRELTYVTPKPATLEDIQGLVRDYAKAAENAIEAGFDGVE
ECCCCEEHHHHHHHCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEE
IHGANGYLIDTFLHHDSNRRTDEYGGTPANMSRFALEVVDAVVARIGKDRTGLRISPGAY
EECCCCEEEEEEEECCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCE
FNMAADSRDREVFDYFLPELESRDIAFVHIGIFDDSMEFDYLGGRASSYVRAHYGKTLVG
EEECCCCCCHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEECCCCHHHHHHHHCCCEEEE
VGSYSAETASEAIAANKFDLIAIGRPFIANPDYVARVRDGVELVPYSDEMLASLV
ECCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]