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The map label for this gene is trmD

Identifier: 126173450

GI number: 126173450

Start: 1392751

End: 1393497

Strand: Direct

Name: trmD

Synonym: Sbal_1212

Alternate gene names: 126173450

Gene position: 1392751-1393497 (Clockwise)

Preceding gene: 126173449

Following gene: 126173451

Centisome position: 27.16

GC content: 49.93

Gene sequence:

>747_bases
ATGTGGTTAGGGGTAATAACCCTGTTCCCAGAGATGTTCCGTGCCGTGACAGACTTTGGAGTTACGGGTCGAGCCGTGAA
AAACGGCCTGCTTGAGTTGCACACGTGGAATCCCCGTGATTTCACCCATGATAGACATAATACGGTAGATGACAGGCCTT
ACGGCGGTGGTCCCGGAATGTTGATGATGGTGCAACCCTTGCGCGATGCCATCCATGCAGCGAAAGCCGCAGCGGGTGAA
GGTGCGAAGGTGATTTACTTGTCACCTCAAGGACGCAAGCTGGATCAGCAAGGCGTCACTGAGTTGGCTCAATCATCACG
TTTGATTTTGGTCTGTGGTCGATACGAAGGTATTGATGAACGCATCATTCAAACGGAAGTGGATGAAGAGTGGTCGATAG
GGGATTACGTGCTTTCGGGCGGCGAATTACCGGCGATGACTCTGATAGATTCAGTATCGAGATTGGTTCCTGGCGTGCTA
GGAAAACAAGCTTCGGCGGAGCAAGATTCTTTCTCCGACGGTTTACTGGATTGTCCACATTACACTCGCCCTGAAAGCCT
TGATGGTGTTGACGTACCGGCAGTGCTGCTAAGTGGCAACCACGAACAAATTAGACTTTGGCGTTTACAGCAAAGCCTTG
GTAGGACTTTACTGAGACGGCCAGAATTATTGCAAAATCTAGCTCTGACTGACGAACAAACGACTCTATTAGCGCAGTTC
GTTGAAGCAATGAACAAGCATGCTTAG

Upstream 100 bases:

>100_bases
GATCCCCTTTGTCCCTGAGCAGTTCATCAAAACGGTGGACCTGCAAGGTAAACAGATTTTAGTGGATTGGGATCCTGACT
TCTAAGTCGAGGTACAACAG

Downstream 100 bases:

>100_bases
TCGTAGCTCAGTTAATTCTAGAACGGAGTAAGTTATGAACAACATCATTAAAATGCTCAACGATGAGCAAATGAAACAAG
ACGTACCTGCGTTTGGTGCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHNTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVTELAQSSRLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGVDVPAVLLSGNHEQIRLWRLQQSLGRTLLRRPELLQNLALTDEQTTLLAQF
VEAMNKHA

Sequences:

>Translated_248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHNTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVTELAQSSRLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGVDVPAVLLSGNHEQIRLWRLQQSLGRTLLRRPELLQNLALTDEQTTLLAQF
VEAMNKHA
>Mature_248_residues
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHNTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGE
GAKVIYLSPQGRKLDQQGVTELAQSSRLILVCGRYEGIDERIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVL
GKQASAEQDSFSDGLLDCPHYTRPESLDGVDVPAVLLSGNHEQIRLWRLQQSLGRTLLRRPELLQNLALTDEQTTLLAQF
VEAMNKHA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=240, Percent_Identity=82.5, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_SHEB2 (B8EBP1)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359009.1
- ProteinModelPortal:   B8EBP1
- SMR:   B8EBP1
- GeneID:   7087879
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_3101
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27427; Mature: 27427

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHNTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQQGVTELAQSSRLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGVDVPAVLLSGNHEQIRLWRLQQSLGRTLLRRPELLQNLALTDEQTTLLAQF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VEAMNKHA
HHHHHCCC
>Mature Secondary Structure
MWLGVITLFPEMFRAVTDFGVTGRAVKNGLLELHTWNPRDFTHDRHNTVDDRPYGGGPGM
CCCHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
LMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQQGVTELAQSSRLILVCGRYEGIDE
EEEHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEECCCCCHHH
RIIQTEVDEEWSIGDYVLSGGELPAMTLIDSVSRLVPGVLGKQASAEQDSFSDGLLDCPH
HHHHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
YTRPESLDGVDVPAVLLSGNHEQIRLWRLQQSLGRTLLRRPELLQNLALTDEQTTLLAQF
CCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VEAMNKHA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA