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The map label for this gene is thyA [H]

Identifier: 126173426

GI number: 126173426

Start: 1371628

End: 1372422

Strand: Direct

Name: thyA [H]

Synonym: Sbal_1188

Alternate gene names: 126173426

Gene position: 1371628-1372422 (Clockwise)

Preceding gene: 126173425

Following gene: 126173427

Centisome position: 26.75

GC content: 46.16

Gene sequence:

>795_bases
ATGAAACAGTATTTAGACTTAATGAAGCACATCTTAGCCGAAGGTGTGGATAAATCGGATCGCACAGGTACGGGGACTCG
CTCAGTATTTGGCTATCAAATGCGCTTCGATTTGAGCAAAGGTTTTCCTTTGGTAAGCACCAAGAAATGCCACATGCGCT
CTATCATCCACGAATTGCTGTGGTTCCTGAAGGGTGAAACTAACGTTGCCTATTTACGTGAAAACAAGGTCAGTATTTGG
GATGAATGGGCCGATGAAAATGGCGATTTAGGGCCGGTTTATGGTGCCCAGTGGCGTAGCTGGCCAACCCAGAGTGGCGA
TGCTATCGATCAGATTTCACAAGTTATTGCCCAGATAAAATCTCAGCCTGATTCTCGCCGCCTTATCGTTTCTGCGTGGA
ACGTGGGCGAGCTGGATAAAATGGCCTTGGCGCCATGCCACGCGTTTTTCCAGTTCTATGTGGCTGATGGCAAATTATCT
TGTCAGCTGTATCAGCGCAGCTGTGATGTGTTTCTAGGTTTGCCATTTAACATCGCCAGTTATGCTTTGTTAACTATGAT
GGTGGCCCAGCAATGTGACTTGGCCTTAGGTGATTTCGTGTGGACGGGTGGTGATACCCATTTATATTCAAACCATATGG
AACAAACGGCATTGCAGTTAAGTCGTGAGCCAATGCCATTGCCAACCATGACGATTTTACGTAGACCTGAATCAATTTTT
GATTATCAATTCGACGATTTCGAGCTAACAAACTACGCTCCACATCCCCATATCAAAGCACCTGTCGCCGTGTAA

Upstream 100 bases:

>100_bases
TGGGACTCTACTGGGGATTGATGACTATGGGGCAGATACTGTCTATCCCTATGGTGTTAATCGGTTTGTACTTGATTTTT
CGTAAGCAAGGTAAGCAGCA

Downstream 100 bases:

>100_bases
CGTGCATTTTTTCGACCAATACCTCGGAATAACCGAGGTGTTAGGTCGAAACTTACGATTATATTTGTTGTTACAATTCG
TCTACACTCAATTCATTATG

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVSTKKCHMRSIIHELLWFLKGETNVAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQISQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPMPLPTMTILRRPESIF
DYQFDDFELTNYAPHPHIKAPVAV

Sequences:

>Translated_264_residues
MKQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVSTKKCHMRSIIHELLWFLKGETNVAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQISQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPMPLPTMTILRRPESIF
DYQFDDFELTNYAPHPHIKAPVAV
>Mature_264_residues
MKQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVSTKKCHMRSIIHELLWFLKGETNVAYLRENKVSIW
DEWADENGDLGPVYGAQWRSWPTQSGDAIDQISQVIAQIKSQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPMPLPTMTILRRPESIF
DYQFDDFELTNYAPHPHIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50.354609929078, Blast_Score=280, Evalue=1e-75,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=78.030303030303, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.643109540636, Blast_Score=258, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324648, Length=280, Percent_Identity=40.7142857142857, Blast_Score=200, Evalue=2e-52,
Organism=Drosophila melanogaster, GI17137556, Length=284, Percent_Identity=50, Blast_Score=280, Evalue=8e-76,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30081; Mature: 30081

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVSTKKCHMRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
WFLKGETNVAYLRENKVSIWDEWADENGDLGPVYGAQWRSWPTQSGDAIDQISQVIAQIK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPMPLPTMTILRRPESIF
HHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHH
DYQFDDFELTNYAPHPHIKAPVAV
CCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLMKHILAEGVDKSDRTGTGTRSVFGYQMRFDLSKGFPLVSTKKCHMRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHHEEEEEEEECCCCCCCCCCCHHHHHHHHHHHH
WFLKGETNVAYLRENKVSIWDEWADENGDLGPVYGAQWRSWPTQSGDAIDQISQVIAQIK
HHHCCCCCEEEEECCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
SQPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCDLALGDFVWTGGDTHLYSNHMEQTALQLSREPMPLPTMTILRRPESIF
HHHHHHHHHHHCCCHHHCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHH
DYQFDDFELTNYAPHPHIKAPVAV
CCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA