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The map label for this gene is mak [H]

Identifier: 126173374

GI number: 126173374

Start: 1308461

End: 1309357

Strand: Direct

Name: mak [H]

Synonym: Sbal_1134

Alternate gene names: 126173374

Gene position: 1308461-1309357 (Clockwise)

Preceding gene: 126173373

Following gene: 126173375

Centisome position: 25.52

GC content: 48.38

Gene sequence:

>897_bases
ATGTTACGAATTGGTATCGATCTTGGCGGCACAAAAATCGAGTTAATTGCATTAGATGACGATGGTAAAGAAGTGTTTCG
TAAACGCATCCCAACGCCAAGAGAATATGTTGCTACAGTCGATGCATTAGTCGCACTCGTGACAGAAGTCGAAGAGAGCT
TGCAACAAACCGCCACAATAGGCATAGGTATTCCTGGGGTGATTTCCCCTTTTACTGGCCTAGTTAAAAATGCCAATTCA
ACGTGGATCAATGGCCATCCCCTCGATGTGGATCTAGGCGCTCGATTAAACCGTAAGGTGAAAATCGCTAATGATGCGAA
CTGTTTTGCCGTATCAGAAGCGGTCGATGGCGCAGCTGCTGGCAAGTCAGTGGTCTTTGGCGCCATTATTGGTACTGGAT
GTGGTGCAGGTATCGCCATCAAAGGCCAAGTGCATGATGGTGGTAATGGTATCGGCGGTGAGTGGGGTCATAATCCTTTA
CCTTGGATGAAAGCCGATGAATTTAATACCACGACGTGTTTTTGTGGTAATAGAGACTGCATCGAGACGTTTATCTCAGG
GACGGGTTTTGTACGCGATTTTCGCGCAGCAGGTGGAGTAGCTGACAGCGGTATTGAAATCGCCCAAATGATGCAAGCAG
GCGATCCGCTGGCGACTCAAGCATTTGATCGCTTTATTGACCGTTTAGCCCGCTCATTGGCCCATGTGATCAATATGATG
GATCCAGATGTTATCGTCCTTGGCGGCGGAGTGTCCAACATCGATGCCATTTACACTGAATTGCCAAAAGTTTTACCGCG
ATATGTGGTTGGCCGTGAGTGTCACACTCCGGTTGTTAAAAACTGTTATGGCGCTTCATCGGGCGTACGTGGTGCCGCTT
GGTTGTGGGGACGCTAA

Upstream 100 bases:

>100_bases
TGACCTGAGGCAGTTTTCAATTACAGTGTATGGCATCAGGCAGTAGAGCCGAGCTTCACTGAGCCGCTTTGCGTTAAATA
TATTGAGATGGAGTAAGTTG

Downstream 100 bases:

>100_bases
CTTTAACCTTAATTTGAAATAGTGTTTGATATTTAAGGCCGAATTCATTTATTTGAATTTGGCCTTTTTTTGATTTTGCC
ATGCGAAAGTTTTACATTTC

Product: fructokinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MLRIGIDLGGTKIELIALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPRYVVGRECHTPVVKNCYGASSGVRGAAWLWGR

Sequences:

>Translated_298_residues
MLRIGIDLGGTKIELIALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPRYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
>Mature_298_residues
MLRIGIDLGGTKIELIALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATIGIGIPGVISPFTGLVKNANS
TWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAAGKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPL
PWMKADEFNTTTCFCGNRDCIETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
DPDVIVLGGGVSNIDAIYTELPKVLPRYVVGRECHTPVVKNCYGASSGVRGAAWLWGR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=299, Percent_Identity=58.8628762541806, Blast_Score=369, Evalue=1e-104,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=38.1270903010033, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790522, Length=298, Percent_Identity=25.503355704698, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786891, Length=275, Percent_Identity=22.5454545454545, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787878, Length=228, Percent_Identity=25, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31483; Mature: 31483

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGIDLGGTKIELIALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATI
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
GIGIPGVISPFTGLVKNANSTWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCEEEEECCCCEEEEHHHHCCHHC
GKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPLPWMKADEFNTTTCFCGNRDC
CCHHEEHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
IETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
HHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
DPDVIVLGGGVSNIDAIYTELPKVLPRYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRIGIDLGGTKIELIALDDDGKEVFRKRIPTPREYVATVDALVALVTEVEESLQQTATI
CEEEEEECCCCEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEE
GIGIPGVISPFTGLVKNANSTWINGHPLDVDLGARLNRKVKIANDANCFAVSEAVDGAAA
CCCCCHHHHHHHHHHHCCCCCEECCCCCEEECCCCCCCEEEEECCCCEEEEHHHHCCHHC
GKSVVFGAIIGTGCGAGIAIKGQVHDGGNGIGGEWGHNPLPWMKADEFNTTTCFCGNRDC
CCHHEEHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
IETFISGTGFVRDFRAAGGVADSGIEIAQMMQAGDPLATQAFDRFIDRLARSLAHVINMM
HHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
DPDVIVLGGGVSNIDAIYTELPKVLPRYVVGRECHTPVVKNCYGASSGVRGAAWLWGR
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]