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The map label for this gene is puuB [H]

Identifier: 126173344

GI number: 126173344

Start: 1269091

End: 1270398

Strand: Reverse

Name: puuB [H]

Synonym: Sbal_1103

Alternate gene names: 126173344

Gene position: 1270398-1269091 (Counterclockwise)

Preceding gene: 126173356

Following gene: 126173334

Centisome position: 24.78

GC content: 49.54

Gene sequence:

>1308_bases
ATGGCAAGCAAACCTCCAGTGCACAGCGAACAGTACCCACACTCATACTATGTTGATACTGCTAAAGAAATTTACGCGCA
CCCACAACTCGAAGGCAATATCAGTGCCGATGTCTGCATAGTCGGCGGCGGCTTTAGCGGTATCAATACCGCCATAGAAC
TCGCACAGCGCGGCATGAGTGTAGTCCTATTAGAAGCCAAACGAATTGGCTGGGGCGCATCGGGGCGAAACGGCGGCGAA
CTCATTCGCGGCATAGGCCATGACCCTTCGCAATTTCACAGTGAAATAGGCACTGAAGGTGTTCAAGCAATCCAACAGAT
GGGCTTTGAAGCCGTCGAAATTGTGCGAAATCGCATACAAGAACACAGTATCGACTGCGATCTGCAAATGGGTTATTGCG
ACTTAGCAGTTAAACCTCGTCACATGGATGAGCTTGAAGAAGAATTTAGCGCACTCAAGGCCATGGGTTATCAGCAAGAA
ATCAAGCTGTTAACTAAGCCACAATTAGGAGAAGTCATAGGCTCAGATTTTTATCAAGGTGCGCTGGTCGATATGGGCAG
CGGCCATTTACACCCGCTGAATCTTGCTCTGGGTGAGGCACGCGTCGCCCGTAATCTGGGCGTGCAGTTGTTTGAATACA
GCGCCGCCGAAAAAATCATTCAAGGCGCTAAACCTAAGGTCATGACCGCCAAGGGCGAAGTCACTTGCCAATACTTAGTA
CTGGCTGGCAATGCGTATATTGGCCACAAACTTAACAGTTTTGTTGGGGGTAAAGTATTACCCGCTGGCAGTTATTTATT
GGCGACCGAGCCCTTAACTGAGGCCCAATGCGCGAGCATTATCCCGCAGAATATGGCCTTCGCTGATATGCGAATTGCCT
TAGATTACTTTCACTTATCCGCAGATCGTCGACTGCTGTTTGGTGGCCTTTGTACTTACTCAGGTAAAGATCCCAAGGAT
ATTGAAGCGGCACTGAGACCGAATCTGGAGAAGGTTTTTCCACAGTTAAAAGGCGTGCGCATCGATTATGAATGGGGCGG
CATGATAGGTATTGGCGCGAACCGTATGCCACAAATTGGTCGCCTGCCCGATGCCAATAATATTTTCTACGCCCAAGCCT
ATGCAGGACATGGGGTGAATGCCACTCATATGGCGGCAAAACTACTGGCCGAGGCTATTTGCCAACAGGCGGGATATTTC
GATGTGTTCGCAAAAATCAAACACATGACCTTCCCCGGCGGCCCGACATTCCGCTCGCCTATGCTCGCGGCTGGCATGCT
GTATCACAGATTTAAAGACTTGTTTTAA

Upstream 100 bases:

>100_bases
GTGTTAAATAAAACGAACACTTTTTTGCGTCATGTTAAAAGTGTGATGAGTATACTGAGACAAGTCCAATTTACAATAAA
AACAATTTTTTGGAGCCATA

Downstream 100 bases:

>100_bases
AAGCTTATTTAAAGCACTTTTTAAAGAACAGATTTTAAAGGCTGGTTTTAAGAACTGGTTTTAAGCCATAACGCATGATG
CCCATCGGATAAACAATCCG

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MASKPPVHSEQYPHSYYVDTAKEIYAHPQLEGNISADVCIVGGGFSGINTAIELAQRGMSVVLLEAKRIGWGASGRNGGE
LIRGIGHDPSQFHSEIGTEGVQAIQQMGFEAVEIVRNRIQEHSIDCDLQMGYCDLAVKPRHMDELEEEFSALKAMGYQQE
IKLLTKPQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARNLGVQLFEYSAAEKIIQGAKPKVMTAKGEVTCQYLV
LAGNAYIGHKLNSFVGGKVLPAGSYLLATEPLTEAQCASIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDANNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYF
DVFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF

Sequences:

>Translated_435_residues
MASKPPVHSEQYPHSYYVDTAKEIYAHPQLEGNISADVCIVGGGFSGINTAIELAQRGMSVVLLEAKRIGWGASGRNGGE
LIRGIGHDPSQFHSEIGTEGVQAIQQMGFEAVEIVRNRIQEHSIDCDLQMGYCDLAVKPRHMDELEEEFSALKAMGYQQE
IKLLTKPQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARNLGVQLFEYSAAEKIIQGAKPKVMTAKGEVTCQYLV
LAGNAYIGHKLNSFVGGKVLPAGSYLLATEPLTEAQCASIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKD
IEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDANNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYF
DVFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF
>Mature_434_residues
ASKPPVHSEQYPHSYYVDTAKEIYAHPQLEGNISADVCIVGGGFSGINTAIELAQRGMSVVLLEAKRIGWGASGRNGGEL
IRGIGHDPSQFHSEIGTEGVQAIQQMGFEAVEIVRNRIQEHSIDCDLQMGYCDLAVKPRHMDELEEEFSALKAMGYQQEI
KLLTKPQLGEVIGSDFYQGALVDMGSGHLHPLNLALGEARVARNLGVQLFEYSAAEKIIQGAKPKVMTAKGEVTCQYLVL
AGNAYIGHKLNSFVGGKVLPAGSYLLATEPLTEAQCASIIPQNMAFADMRIALDYFHLSADRRLLFGGLCTYSGKDPKDI
EAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIGRLPDANNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFD
VFAKIKHMTFPGGPTFRSPMLAAGMLYHRFKDLF

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=423, Percent_Identity=42.080378250591, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47468; Mature: 47337

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKPPVHSEQYPHSYYVDTAKEIYAHPQLEGNISADVCIVGGGFSGINTAIELAQRGMS
CCCCCCCCCCCCCCCEEHHCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCE
VVLLEAKRIGWGASGRNGGELIRGIGHDPSQFHSEIGTEGVQAIQQMGFEAVEIVRNRIQ
EEEEEEHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHH
EHSIDCDLQMGYCDLAVKPRHMDELEEEFSALKAMGYQQEIKLLTKPQLGEVIGSDFYQG
HCCCCCEEEECEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCHHHCC
ALVDMGSGHLHPLNLALGEARVARNLGVQLFEYSAAEKIIQGAKPKVMTAKGEVTCQYLV
CEEECCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEE
LAGNAYIGHKLNSFVGGKVLPAGSYLLATEPLTEAQCASIIPQNMAFADMRIALDYFHLS
EECCCCCCCHHHHHCCCEEECCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHC
ADRRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCCC
RLPDANNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFDVFAKIKHMTFPGGPTFRSP
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCH
MLAAGMLYHRFKDLF
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
ASKPPVHSEQYPHSYYVDTAKEIYAHPQLEGNISADVCIVGGGFSGINTAIELAQRGMS
CCCCCCCCCCCCCCEEHHCHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCE
VVLLEAKRIGWGASGRNGGELIRGIGHDPSQFHSEIGTEGVQAIQQMGFEAVEIVRNRIQ
EEEEEEHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHH
EHSIDCDLQMGYCDLAVKPRHMDELEEEFSALKAMGYQQEIKLLTKPQLGEVIGSDFYQG
HCCCCCEEEECEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHCCHHHCC
ALVDMGSGHLHPLNLALGEARVARNLGVQLFEYSAAEKIIQGAKPKVMTAKGEVTCQYLV
CEEECCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEE
LAGNAYIGHKLNSFVGGKVLPAGSYLLATEPLTEAQCASIIPQNMAFADMRIALDYFHLS
EECCCCCCCHHHHHCCCEEECCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHC
ADRRLLFGGLCTYSGKDPKDIEAALRPNLEKVFPQLKGVRIDYEWGGMIGIGANRMPQIG
CCCEEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHCCEEEEEECCCEEEECCCCCCCCC
RLPDANNIFYAQAYAGHGVNATHMAAKLLAEAICQQAGYFDVFAKIKHMTFPGGPTFRSP
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCCCCH
MLAAGMLYHRFKDLF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]