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The map label for this gene is hprA [H]

Identifier: 126173229

GI number: 126173229

Start: 1128716

End: 1129669

Strand: Direct

Name: hprA [H]

Synonym: Sbal_0985

Alternate gene names: 126173229

Gene position: 1128716-1129669 (Clockwise)

Preceding gene: 126173228

Following gene: 126173234

Centisome position: 22.01

GC content: 49.79

Gene sequence:

>954_bases
ATGAAGATAGTTGTACTCGATGGCGAAACCTTAAATCCTGGCGATCTCAGTTGGCAATCCTTGACTGAATTAGGCGAGTT
TGCGTGTTTCCCCCGCACCGATGGCGCCGATATTGTCGCGCGTGCCCGAGGTGCAGAAATCATTCTCACCAATAAAACGC
CGCTTAATGCTAAGACTCTGGCGCAACTTCCGGCACTGAAATACATAGGCGTATTGGCAACGGGTACTAACGTTGTCGAT
ATAGCGGCTGCAAAAGCGCGCGGTATAGTGGTTACGAATGTACCCGCCTATGCACCCGATGCCGTAGCGCAGATGGTCTT
TGCCCATATACTGCATCACACCCAAGCTGTGGCTGCCCATCATCAAGCCGTGGTCGATGGTCAGTGGGTGAACTGCCAAG
ATTTTTGTTTCACCTTAATGCCGCTGCAATCCTTAAAAGGCAAGACTTTAGGTTTAATAGGTTATGGTGATATCGGCCAG
CAGGTTGGGACGATCGCCCTTGCATTCGGGATGAAAGTGCTCGTCAATACCCGCTCTGTTATCAAAGACTTGCCCCAAGG
TGCCACTTGGGTACCGCGTGATACTGTGCTGAGCGAATCAGATATTATCTCGCTGCATTGCCCGCTCACTCCTGAAACTG
AGCAATTTATCAATCAACAAACTCTTGCTTTGATGAAGCCTAATGCGCTGCTTATTAATACGGCACGCGGTGGATTAATT
GATGAGGTCGCGCTTGCTGCTGCGCTTTCACAAAACCGTTTATTTGCCGGTGTCGATGTGTTATCGACCGAGCCGCCCAG
TGCGGATAATCCCTTATTGCATGCACCGAATATCAGCATTAGTCCCCACAACGCGTGGGCGACTAAGGAAGCGAGACAAA
GTCTTCTCAATATTGCCGTGGCGAATGTGCACGGTTATTTGCAGGGTGAAGTGATTAACTGCGTGAACAAGTAA

Upstream 100 bases:

>100_bases
GGCACATTACGTCGGATGTGATGTGGCAGGCGCAGGCTAACCTTGGGCTCAAAGTCAGTTTTTAGCGCTAACACATCGTC
TTAAAAGGAGATTTAGCGGC

Downstream 100 bases:

>100_bases
AGCGGTATGCGTCTTTCTCTCTTTTCATCATATCGACGTTGAGAGAGAAAGACCTGATGGTTAGTTATTTTTAGTCTTTC
TTCTACGCATCCACCACACA

Product: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTDGADIVARARGAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGQWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTIALAFGMKVLVNTRSVIKDLPQGATWVPRDTVLSESDIISLHCPLTPETEQFINQQTLALMKPNALLINTARGGLI
DEVALAAALSQNRLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVNK

Sequences:

>Translated_317_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTDGADIVARARGAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGQWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTIALAFGMKVLVNTRSVIKDLPQGATWVPRDTVLSESDIISLHCPLTPETEQFINQQTLALMKPNALLINTARGGLI
DEVALAAALSQNRLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVNK
>Mature_317_residues
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTDGADIVARARGAEIILTNKTPLNAKTLAQLPALKYIGVLATGTNVVD
IAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAHHQAVVDGQWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVGTIALAFGMKVLVNTRSVIKDLPQGATWVPRDTVLSESDIISLHCPLTPETEQFINQQTLALMKPNALLINTARGGLI
DEVALAAALSQNRLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAVANVHGYLQGEVINCVNK

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=274, Percent_Identity=35.036496350365, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI4557499, Length=274, Percent_Identity=35.036496350365, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI61743967, Length=281, Percent_Identity=33.8078291814947, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI4557497, Length=281, Percent_Identity=33.8078291814947, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI145580575, Length=274, Percent_Identity=35.036496350365, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI23308577, Length=260, Percent_Identity=30.7692307692308, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI6912396, Length=251, Percent_Identity=29.0836653386454, Blast_Score=98, Evalue=8e-21,
Organism=Escherichia coli, GI87082289, Length=288, Percent_Identity=34.7222222222222, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1789279, Length=283, Percent_Identity=33.9222614840989, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787645, Length=270, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87081824, Length=158, Percent_Identity=31.6455696202532, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=274, Percent_Identity=29.5620437956204, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25147481, Length=275, Percent_Identity=28.7272727272727, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6320925, Length=282, Percent_Identity=32.2695035460993, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=284, Percent_Identity=32.0422535211268, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=325, Percent_Identity=25.8461538461538, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324964, Length=252, Percent_Identity=28.1746031746032, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321253, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571528, Length=288, Percent_Identity=34.0277777777778, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI28574286, Length=287, Percent_Identity=32.0557491289199, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=32.2695035460993, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=32.2695035460993, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585514, Length=282, Percent_Identity=32.2695035460993, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28574282, Length=282, Percent_Identity=32.2695035460993, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=32.2695035460993, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24646446, Length=274, Percent_Identity=32.8467153284672, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646448, Length=274, Percent_Identity=32.8467153284672, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646452, Length=274, Percent_Identity=32.8467153284672, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24646450, Length=274, Percent_Identity=32.8467153284672, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI62472511, Length=274, Percent_Identity=32.8467153284672, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24585516, Length=279, Percent_Identity=28.3154121863799, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=28.8537549407115, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 33766; Mature: 33766

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTDGADIVARARGAEIILTNKTPLNAKTL
CEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHEECCCCEEEEECCCCCCHHHH
AQLPALKYIGVLATGTNVVDIAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAH
HHCCHHHHEEEEECCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVVDGQWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQQVGTIALAFGMKVLVNTRSV
HHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHH
IKDLPQGATWVPRDTVLSESDIISLHCPLTPETEQFINQQTLALMKPNALLINTARGGLI
HHHCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHCHHHHEEECCCEEEEECCCCCHH
DEVALAAALSQNRLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAV
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANVHGYLQGEVINCVNK
HHHCCEECCHHHHHCCC
>Mature Secondary Structure
MKIVVLDGETLNPGDLSWQSLTELGEFACFPRTDGADIVARARGAEIILTNKTPLNAKTL
CEEEEECCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHEECCCCEEEEECCCCCCHHHH
AQLPALKYIGVLATGTNVVDIAAAKARGIVVTNVPAYAPDAVAQMVFAHILHHTQAVAAH
HHCCHHHHEEEEECCCCEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVVDGQWVNCQDFCFTLMPLQSLKGKTLGLIGYGDIGQQVGTIALAFGMKVLVNTRSV
HHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHH
IKDLPQGATWVPRDTVLSESDIISLHCPLTPETEQFINQQTLALMKPNALLINTARGGLI
HHHCCCCCCCCCCHHCCCCCCEEEEECCCCCCHHHHHCHHHHEEECCCEEEEECCCCCHH
DEVALAAALSQNRLFAGVDVLSTEPPSADNPLLHAPNISISPHNAWATKEARQSLLNIAV
HHHHHHHHHHCCCEEEEEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANVHGYLQGEVINCVNK
HHHCCEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]