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The map label for this gene is prpB [C]

Identifier: 126173124

GI number: 126173124

Start: 1000740

End: 1001588

Strand: Direct

Name: prpB [C]

Synonym: Sbal_0879

Alternate gene names: 126173124

Gene position: 1000740-1001588 (Clockwise)

Preceding gene: 126173123

Following gene: 126173126

Centisome position: 19.52

GC content: 47.82

Gene sequence:

>849_bases
ATGGCTGAGCAATTGAGAATACTTTTCAAAGCACTACACCAACAATCAGAGCCGCTGATATTAACCAATGTTTGGGATGC
TGCCAGTGCGGCCATCATCCAAGCATCGGGCGCCCGTGCGATTGCGACCAGTAGCGCGGCATTGGCTTGGTCGCTCGGTT
ATCCCGATGGCCAAGCGCTACCTAAAGCGGCTTTATTGGACGCAGTAAACAATATCCTACGGTTGAGCCGAGTGCCCGTG
ACGATAGATATTGAATCTGGTTATAGCCAAGATCCCGATGAGGTTACTGCTTTTGTTGCACAGCTCGCTGAGCTTGGCGT
AGCAGGGATTAATATTGAGGATGGCAGTGCAGGTGTTGCTGAGATGGTGGCAAAAATTAAAGCTATTCGCGTCCATCCTC
GTTGCCAAGGATTATTTATTAATGCGCGAACCGATGTGTATTTACTGGGCTTAGCCAGTGGTGATGCCGCGGTCGCTATG
ACTATCGATAGGTTAACTCAGTATCAGGCTGCGGGCGCGGATGGCGGCTTTATCCCTGGCGCGAATCAGGTCGAGACCGC
TAAGCGCTTGTCTGCCGCTGTGGATATGCCACTGAATTTTATGCTATTGAATGAGCAGATGGATATCGGCGAGTTATTTG
CCGCAGGTGTGCAGCGTTTTTCCACCGGTCCGGCTTCATTTTTACAAGCCTATGGCACTTTGCTTAATCCAATTTTGTTA
AATTCAATTAAGGCAAAGCCGTTTCAGCAACAGCCAAAAGATGCGGTTATTGCCGCTGATAATGGCTTCATATATAAAGT
CATTGCCCTTGATTTTGAACGAATGAATAAATTATTTATCCCGAGTTAA

Upstream 100 bases:

>100_bases
AGCCTTGAGCCCTTGGCTTTTAGGTTCAGTGCCGTTTTCCGCGATAATGACCTAAGCCATCTTGTTAGAGTTGAGTTTTC
AACACCAACAAGGACATAAG

Downstream 100 bases:

>100_bases
AGCTGTTTTTCATCTATACCCAAGCTACCTGAAGATACGAGTTTCAGAGCGCCCAGGGTGCTTCAATTCAAGGCGCATTA
ATGACAGAATGTCGGCCACC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAEQLRILFKALHQQSEPLILTNVWDAASAAIIQASGARAIATSSAALAWSLGYPDGQALPKAALLDAVNNILRLSRVPV
TIDIESGYSQDPDEVTAFVAQLAELGVAGINIEDGSAGVAEMVAKIKAIRVHPRCQGLFINARTDVYLLGLASGDAAVAM
TIDRLTQYQAAGADGGFIPGANQVETAKRLSAAVDMPLNFMLLNEQMDIGELFAAGVQRFSTGPASFLQAYGTLLNPILL
NSIKAKPFQQQPKDAVIAADNGFIYKVIALDFERMNKLFIPS

Sequences:

>Translated_282_residues
MAEQLRILFKALHQQSEPLILTNVWDAASAAIIQASGARAIATSSAALAWSLGYPDGQALPKAALLDAVNNILRLSRVPV
TIDIESGYSQDPDEVTAFVAQLAELGVAGINIEDGSAGVAEMVAKIKAIRVHPRCQGLFINARTDVYLLGLASGDAAVAM
TIDRLTQYQAAGADGGFIPGANQVETAKRLSAAVDMPLNFMLLNEQMDIGELFAAGVQRFSTGPASFLQAYGTLLNPILL
NSIKAKPFQQQPKDAVIAADNGFIYKVIALDFERMNKLFIPS
>Mature_281_residues
AEQLRILFKALHQQSEPLILTNVWDAASAAIIQASGARAIATSSAALAWSLGYPDGQALPKAALLDAVNNILRLSRVPVT
IDIESGYSQDPDEVTAFVAQLAELGVAGINIEDGSAGVAEMVAKIKAIRVHPRCQGLFINARTDVYLLGLASGDAAVAMT
IDRLTQYQAAGADGGFIPGANQVETAKRLSAAVDMPLNFMLLNEQMDIGELFAAGVQRFSTGPASFLQAYGTLLNPILLN
SIKAKPFQQQPKDAVIAADNGFIYKVIALDFERMNKLFIPS

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 30001; Mature: 29870

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQLRILFKALHQQSEPLILTNVWDAASAAIIQASGARAIATSSAALAWSLGYPDGQAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHEEEECCCCCEEEECCCEEEEEECCCCCCCC
PKAALLDAVNNILRLSRVPVTIDIESGYSQDPDEVTAFVAQLAELGVAGINIEDGSAGVA
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHH
EMVAKIKAIRVHPRCQGLFINARTDVYLLGLASGDAAVAMTIDRLTQYQAAGADGGFIPG
HHHHHHHHEEECCCCCEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCC
ANQVETAKRLSAAVDMPLNFMLLNEQMDIGELFAAGVQRFSTGPASFLQAYGTLLNPILL
CCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NSIKAKPFQQQPKDAVIAADNGFIYKVIALDFERMNKLFIPS
HHCCCCCCCCCCCCEEEEECCCEEEEEEEHHHHHHCCCCCCC
>Mature Secondary Structure 
AEQLRILFKALHQQSEPLILTNVWDAASAAIIQASGARAIATSSAALAWSLGYPDGQAL
CHHHHHHHHHHHCCCCCEEEEECCCCHHHEEEECCCCCEEEECCCEEEEEECCCCCCCC
PKAALLDAVNNILRLSRVPVTIDIESGYSQDPDEVTAFVAQLAELGVAGINIEDGSAGVA
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHH
EMVAKIKAIRVHPRCQGLFINARTDVYLLGLASGDAAVAMTIDRLTQYQAAGADGGFIPG
HHHHHHHHEEECCCCCEEEEECCCCEEEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCCC
ANQVETAKRLSAAVDMPLNFMLLNEQMDIGELFAAGVQRFSTGPASFLQAYGTLLNPILL
CCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
NSIKAKPFQQQPKDAVIAADNGFIYKVIALDFERMNKLFIPS
HHCCCCCCCCCCCCEEEEECCCEEEEEEEHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]