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The map label for this gene is pitA [H]

Identifier: 126173123

GI number: 126173123

Start: 999136

End: 1000605

Strand: Direct

Name: pitA [H]

Synonym: Sbal_0878

Alternate gene names: 126173123

Gene position: 999136-1000605 (Clockwise)

Preceding gene: 126173122

Following gene: 126173124

Centisome position: 19.49

GC content: 48.84

Gene sequence:

>1470_bases
ATGTTTGAAATGTTCACAACGTTAGGCTTTGCTTGGTCTGTGGGTTTAGTGCTTGCGATACTGTTTGTATTGGCATACGA
ATTCATTAATGGCTTCCACGACACGGCCAATGCGGTGGCTACGGTTATTTATACCAAGGCTATGCCAGCCAATCTTGCGG
TCGTTACCTCTGCGCTCTTTAACTTTGCAGGTGTGCTATTAGGTGGCTTAGGTGTGGCCTATGCGATAGTGCATTTATTG
CCCGTAGATTCCTTGCTCGGCATGGATTCGACCCAAGGTTTATTGATGGTCTTTTCTTTGCTGTTTTCGGCAATTATCTG
GAACTTAGGCACTTGGTATTTTGGTATACCGGCATCGAGTTCCCATACCTTGATTGGCTCGATAATGGGCGTGGGCGGCG
CGTTCGCTTGGATGAATCATCAGCCTATCTTGCAGGGTATTAATGTCTCTAAGGCCTCGCAGATCATGTTGTCCTTAATC
ATTTCGCCGACTTTAGGTTTTGTGATGGCGGGGATTTTTCTACTCATCATGAAATACGTCTGGCAGAAACATAAAATTCA
CCGCACTCCCGATGAGCAGATGCAGATTAATGGCAAAAAACACCCGCCATTTTGGGCAAGGGCGAGTTTAATTGTCTCTG
CTATGGGGGTGAGTTTTGCCCATGGTTCGAACGATGGCCAGAAGGGTATAGGCCTAGTGATGCTAGTGCTGATTTGTATG
GCACCGGCGTATTTTGCCTTGGATATGGGCAGTCAACCCTATGATTTGGAAAGAACTCAAGATGCCAATCGGCGCATTAT
GGCGATTTACGATCGTAACCAAGCCGTGATCGCTGATGTGATCGACTTTAGCGCTTCAGCAAACGTCCCCGAATATATGC
TGGCCCATTGCTCATCTGAAAATGTATTACCTGCGATGTCGCTACTTGACCGCCGCTTGGCAAAAGTCTCGACTTACAAA
GACATGGATGTACAAGAGCGGCGCGAGGTGCGTCGGTTACTCCTGTGTATCGATGATACTGCCCGCCAAGTGGCGAAGTT
AGATCTCCCTGCTAAAGATCTGACTTCATTGGCTAAATGGCGCAAGGATCTCACCCGCACCACTGAATATGCGCCACTTT
GGGTGATTATTGCCATTGCGACTGCGTTGGGTTGCGGCACGTTAGTGGGCTGGCGACGGATTGTGTATACCGTGGGGGAG
AAAATTGGTTCATCTAGTATGACCTACAGCCAAGGCATTGCCGCACAGGTCACCGCTGCCGTCTCTATTGGGATTGCCAG
TATGACGGGCATGCCAGTGTCGACGACTCATATTCTATCTTCCGCCGTTGCGGGCACTATGGTGGCTAATCGTTCGGGGC
TTCAGATCCAAACAATTAAGCAAATCATGCTCGCTTGGCTGCTGACGCTTCCTATTACTATGTTGCTCTCTGCGTGCGTG
TTTATCCTTGCCAATGCACTTTGGGGTTAG

Upstream 100 bases:

>100_bases
AGGACTAATTCACTTTTTGACAATCTAAATAATTTTTCACTGTAAAAGAGGCCAAACTTGGGCGTAATCTGTGGGCGTTC
TCGCCATGGGTCAGTATTTT

Downstream 100 bases:

>100_bases
CCAAGAATACCCAAGTTGAATGCTCACATAGCCAAGCCTTGAGCCCTTGGCTTTTAGGTTCAGTGCCGTTTTCCGCGATA
ATGACCTAAGCCATCTTGTT

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MFEMFTTLGFAWSVGLVLAILFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWMNHQPILQGINVSKASQIMLSLI
ISPTLGFVMAGIFLLIMKYVWQKHKIHRTPDEQMQINGKKHPPFWARASLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMGSQPYDLERTQDANRRIMAIYDRNQAVIADVIDFSASANVPEYMLAHCSSENVLPAMSLLDRRLAKVSTYK
DMDVQERREVRRLLLCIDDTARQVAKLDLPAKDLTSLAKWRKDLTRTTEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSSMTYSQGIAAQVTAAVSIGIASMTGMPVSTTHILSSAVAGTMVANRSGLQIQTIKQIMLAWLLTLPITMLLSACV
FILANALWG

Sequences:

>Translated_489_residues
MFEMFTTLGFAWSVGLVLAILFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWMNHQPILQGINVSKASQIMLSLI
ISPTLGFVMAGIFLLIMKYVWQKHKIHRTPDEQMQINGKKHPPFWARASLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMGSQPYDLERTQDANRRIMAIYDRNQAVIADVIDFSASANVPEYMLAHCSSENVLPAMSLLDRRLAKVSTYK
DMDVQERREVRRLLLCIDDTARQVAKLDLPAKDLTSLAKWRKDLTRTTEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSSMTYSQGIAAQVTAAVSIGIASMTGMPVSTTHILSSAVAGTMVANRSGLQIQTIKQIMLAWLLTLPITMLLSACV
FILANALWG
>Mature_489_residues
MFEMFTTLGFAWSVGLVLAILFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALFNFAGVLLGGLGVAYAIVHLL
PVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASSSHTLIGSIMGVGGAFAWMNHQPILQGINVSKASQIMLSLI
ISPTLGFVMAGIFLLIMKYVWQKHKIHRTPDEQMQINGKKHPPFWARASLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
APAYFALDMGSQPYDLERTQDANRRIMAIYDRNQAVIADVIDFSASANVPEYMLAHCSSENVLPAMSLLDRRLAKVSTYK
DMDVQERREVRRLLLCIDDTARQVAKLDLPAKDLTSLAKWRKDLTRTTEYAPLWVIIAIATALGCGTLVGWRRIVYTVGE
KIGSSSMTYSQGIAAQVTAAVSIGIASMTGMPVSTTHILSSAVAGTMVANRSGLQIQTIKQIMLAWLLTLPITMLLSACV
FILANALWG

Specific function: Low-affinity inorganic phosphate transport. Can also transport arsenate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=470, Percent_Identity=51.9148936170213, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789360, Length=470, Percent_Identity=50.4255319148936, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI25146401, Length=489, Percent_Identity=23.721881390593, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 53138; Mature: 53138

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEMFTTLGFAWSVGLVLAILFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NFAGVLLGGLGVAYAIVHLLPVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSIMGVGGAFAWMNHQPILQGINVSKASQIMLSLIISPTLGFVMAGIFLLIMKYV
CCHHHHHHHHCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKHKIHRTPDEQMQINGKKHPPFWARASLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
HHHHCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
APAYFALDMGSQPYDLERTQDANRRIMAIYDRNQAVIADVIDFSASANVPEYMLAHCSSE
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCC
NVLPAMSLLDRRLAKVSTYKDMDVQERREVRRLLLCIDDTARQVAKLDLPAKDLTSLAKW
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKDLTRTTEYAPLWVIIAIATALGCGTLVGWRRIVYTVGEKIGSSSMTYSQGIAAQVTAA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHH
VSIGIASMTGMPVSTTHILSSAVAGTMVANRSGLQIQTIKQIMLAWLLTLPITMLLSACV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FILANALWG
HHHHHHHCC
>Mature Secondary Structure
MFEMFTTLGFAWSVGLVLAILFVLAYEFINGFHDTANAVATVIYTKAMPANLAVVTSALF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
NFAGVLLGGLGVAYAIVHLLPVDSLLGMDSTQGLLMVFSLLFSAIIWNLGTWYFGIPASS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCC
SHTLIGSIMGVGGAFAWMNHQPILQGINVSKASQIMLSLIISPTLGFVMAGIFLLIMKYV
CCHHHHHHHHCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WQKHKIHRTPDEQMQINGKKHPPFWARASLIVSAMGVSFAHGSNDGQKGIGLVMLVLICM
HHHHCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
APAYFALDMGSQPYDLERTQDANRRIMAIYDRNQAVIADVIDFSASANVPEYMLAHCSSE
HHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCCCC
NVLPAMSLLDRRLAKVSTYKDMDVQERREVRRLLLCIDDTARQVAKLDLPAKDLTSLAKW
CCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
RKDLTRTTEYAPLWVIIAIATALGCGTLVGWRRIVYTVGEKIGSSSMTYSQGIAAQVTAA
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHH
VSIGIASMTGMPVSTTHILSSAVAGTMVANRSGLQIQTIKQIMLAWLLTLPITMLLSACV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FILANALWG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]