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The map label for this gene is prfB [H]

Identifier: 126173117

GI number: 126173117

Start: 988918

End: 989976

Strand: Direct

Name: prfB [H]

Synonym: Sbal_0872

Alternate gene names: 126173117

Gene position: 988918-989976 (Clockwise)

Preceding gene: 126173115

Following gene: 126173118

Centisome position: 19.29

GC content: 50.14

Gene sequence:

>1059_bases
TTGCCGAGCGCACGCAGCTTCTTAGGGGGTATCTTTGACTACGATGCCAAAAATGAGCGTCTAGAAGAAGTTACCCGTGA
GCTTGAAAGTTCAGAAGTGTGGAATGACCCAGAGCGCGCCCAAGCCTTAGGCAAAGAGCGCGCCAGCCTAGAAGCTGTGG
TCAAAACCATAGACGATATGGACTCAGGTCTTGAAGATGTTGAAGGCTTACTCGAGCTGGCTATTGAAGAGGAAGACGAA
GACACTTTCAACGAAACCAGTAAAGAGCTGGATTATCTCGAAAGCCGCCTTGCCGATCTCGAATTCCGCCGTATGTTCTC
CGGCCCGCAAGATGCGTCAAACTGTTATTTAGACATTCAGTCTGGCAGTGGTGGTACCGAAGCCCAAGATTGGGCCAACA
TGGTGCTGCGTATGTACCTGCGTTGGGGCGAAGCCCATGGTTTTAGCCCAGAGTTGATGGAAGTGACCGATGGTGACGTG
GCCGGTATTAAAGGCGCGACCATTAAGTTCACTGGCGAATATGCGTTTGGTTGGTTACGTACCGAAACGGGCGTGCACCG
CTTAGTGCGTAAGTCGCCATTCGACTCATCGGGTCGTCGTCATACGTCATTCTGCTCTGTGTTCGTTTATCCAGAAATCG
ACGACGATATCACAATCGATATCAACCCATCGGATCTGCGTATCGACACTTACCGCGCTTCGGGCGCGGGTGGTCAGCAC
GTTAACAAAACAGAATCGGCGATTCGTATTACCCACTTACCGACTAACACAGTCGTGCAGTGTCAGAACGACCGTTCACA
GCACAAGAACAAAGATGCTGCGATGAAGCAGCTGAAAGCAAAACTGTTTGAGTTAGAAATGCTCAAGCAAAATGCCGACA
AACAGGCCGCTGAAGACGCTAAGTCTGATATCGGCTGGGGCAGTCAAATTCGCTCTTATGTATTAGACGATGCCCGCATT
AAAGACTTACGCACAGGCGTTGAAAACCGTAGCACTCAAGCGGTACTCGATGGCGATCTGGATAAGTTTATCGAAGCCAG
TTTGAAATCTGGGCTGTAA

Upstream 100 bases:

>100_bases
TCATCGCCTCTTTTAGTGGAAGATCAATTAAAGCGGCCAAATAAAGAAAGTTGAAAATTCATGTTTGAAGTTAATCCAGT
AAAATTCAAAATTAAGGAGC

Downstream 100 bases:

>100_bases
TTAATTCAAGGTATAGACGAGAAATAAGATGACTGAACAAGTAATTGATGAGAACAAACTGATCGCCGAGCGCCGTGCCA
AGCTAGAAAGCATCCGCCCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDE
DTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARI
KDLRTGVENRSTQAVLDGDLDKFIEASLKSGL

Sequences:

>Translated_352_residues
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDE
DTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDV
AGIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARI
KDLRTGVENRSTQAVLDGDLDKFIEASLKSGL
>Mature_351_residues
PSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDMDSGLEDVEGLLELAIEEEDED
TFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQSGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVA
GIKGATIKFTGEYAFGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQHV
NKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDAKSDIGWGSQIRSYVLDDARIK
DLRTGVENRSTQAVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=311, Percent_Identity=32.7974276527331, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI34577120, Length=364, Percent_Identity=29.9450549450549, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI166795305, Length=218, Percent_Identity=33.9449541284404, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI2367172, Length=345, Percent_Identity=75.9420289855072, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=38.150289017341, Blast_Score=207, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=278, Percent_Identity=29.4964028776978, Blast_Score=114, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=317, Percent_Identity=31.8611987381703, Blast_Score=139, Evalue=6e-34,
Organism=Drosophila melanogaster, GI19921226, Length=281, Percent_Identity=36.6548042704626, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39412; Mature: 39281

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDM
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAIEEEDEDTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCC
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSDIGWGSQIRSYVLDDARIKDLRTGVENRSTQAVLDGDLDKFIEASLKSGL
HHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PSARSFLGGIFDYDAKNERLEEVTRELESSEVWNDPERAQALGKERASLEAVVKTIDDM
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
DSGLEDVEGLLELAIEEEDEDTFNETSKELDYLESRLADLEFRRMFSGPQDASNCYLDIQ
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
SGSGGTEAQDWANMVLRMYLRWGEAHGFSPELMEVTDGDVAGIKGATIKFTGEYAFGWLR
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCCCEEEEECCEEEEEEE
TETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDITIDINPSDLRIDTYRASGAGGQH
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEEEEEECCCCCCCC
VNKTESAIRITHLPTNTVVQCQNDRSQHKNKDAAMKQLKAKLFELEMLKQNADKQAAEDA
CCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
KSDIGWGSQIRSYVLDDARIKDLRTGVENRSTQAVLDGDLDKFIEASLKSGL
HHCCCHHHHHHHHHHCHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA