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The map label for this gene is braB [H]

Identifier: 126173087

GI number: 126173087

Start: 951557

End: 952957

Strand: Direct

Name: braB [H]

Synonym: Sbal_0842

Alternate gene names: 126173087

Gene position: 951557-952957 (Clockwise)

Preceding gene: 126173080

Following gene: 126173088

Centisome position: 18.56

GC content: 47.47

Gene sequence:

>1401_bases
GTGCAAGACAATCAGATGAGTATCGCAGATACATTGGGCTTAGGTTTTATGACCTTCGCATTCTTTTTAGGGGCAGGTAA
CCTAATTTTTCCACCGTTCGCGGGAATGTTAGCGGGCGGTAATATGCCCCTCGCCATGTTGGGTTTTTTGATTACCGCCG
TAGGTTTACCTTTAGTTGGACTGATTGCTGTCGCTAAGGCTCAAGGTAAGGTTATGGCTATGCTGCCAGTATTTGCAGCA
ACGACATTGGCTATCGCGATTTATATCATTATCGGCCCTGCGTTTGCTGCGCCGCGTACCGGTTTAGTGGCTTACGAGAT
TGGCGCTAAGCCCTTTATCGATGACACCACGGCCACATTTTTACTGGGCAGTTTAGAACTGAATCTGTCGCAACTGCTAT
ACACCATGTGTTTCTTTACCGTCACTATGTTATTGGCGCTTTTCCCTGGCAAGCTGCTCGATAGCGTAGGTAAAGTATTA
ACGCCTATTTTATTGATCTTGTTAGTTGGATTGGCTGCATCTGTGATGTTTTTACCCGCATCCGAAGTGGGCCAAGCCGT
AGGCGATTATCAAAACCATCCGCTGACCAAAGGTATTTTGGAAGGCTACAACACTATGGATACGTTAGCGTCCTTGATGT
TTGGTATGCTGATTATCGATATCCTGCGCAAAAAGGGGATTGATCAACCGAGTGCTCAAACTAAATATCTGATCCGCGCC
GCCTTTATCGCTGCCGCTGGTTTAGCCTTTGTGTATGTGTCGCTGTTCTTTTTAGGTGCGACCGCAGGCGATATCGCCAT
CGGTGCTAAAAATGGCGGCGAGATTTTAACGAACTATGTGACCCGTGAATTTGGTGATTTAGGCTCAGTCTTACTGTCAT
CGGTCGTGACCTTAGCTTGTTTAACCACAGCGGTAGGCCTTGTAAGTGCATGTTCTGAGTTCTTCAATGAGCTGATGCCT
AAACTGTCTTACCGTTTCCTAGTGATCTTCTTAAGCGTGATTTGTGCTGTGATTGCTAACGTGGGCCTTACGCAGCTTAT
CGCCATCAGTATTCCTGTGTTGATGACGATTTATCCTGTGGCAATCGCCTTAGTGGTCATCACTTTCTTGACCGCTTACT
TTGCGAATCCGCAGTTTGCACACCGTGCAGTACTGAGCGTGGCACTGTTTTTCGGCATCTTCGATGGCCTTAAAGTGGCG
GCGAGCAGTTTGACTGGCGCTGATGGTCAAGCGGTGAATAGCTTAGCGCAGGGCTTTATCCATGTCGTGGATGTGATGTC
TCCCGTGTTATCTAAGCTGCCACTCTATGAAGAAGGCATGGCTTGGTTACTGCCAACGATTGCAACCATAGTACTGTGTT
TAGTGTTTGGTCGTGGTCGTGATCAAACGGCTGCTGCTTAA

Upstream 100 bases:

>100_bases
CAAAGATTTGCACTCCGGATGGGCTTTGTTTTAGCTATGGCTAATCGACACATAATCACTTAAGTTTGTAAAAAAACAAT
AAGACAAGATGGGGCTTCCA

Downstream 100 bases:

>100_bases
GCCTTGTACTCGCGTTTTTACGCGGGACTCAGTTAAGCTGATGTAGGCTTAAATGTAGTTAAGTTAATATCAACGGCGTA
TCATTTGATGACGCCGTTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVL
TPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVA
ASSLTGADGQAVNSLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA

Sequences:

>Translated_466_residues
MQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVL
TPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVA
ASSLTGADGQAVNSLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
>Mature_466_residues
MQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVGLIAVAKAQGKVMAMLPVFAA
TTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATFLLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVL
TPILLILLVGLAASVMFLPASEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYLIRA
AFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLACLTTAVGLVSACSEFFNELMP
KLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPVAIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVA
ASSLTGADGQAVNSLAQGFIHVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=468, Percent_Identity=39.5299145299145, Blast_Score=332, Evalue=3e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 49062; Mature: 49062

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LIAVAKAQGKVMAMLPVFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEECCCCCCCCCHHHHH
LLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVLTPILLILLVGLAASVMFLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYLIRA
HHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLAC
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSEFFNELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVAASSLTGADGQAVNSLAQGFI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
HHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MQDNQMSIADTLGLGFMTFAFFLGAGNLIFPPFAGMLAGGNMPLAMLGFLITAVGLPLVG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LIAVAKAQGKVMAMLPVFAATTLAIAIYIIIGPAFAAPRTGLVAYEIGAKPFIDDTTATF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCEEEEECCCCCCCCCHHHHH
LLGSLELNLSQLLYTMCFFTVTMLLALFPGKLLDSVGKVLTPILLILLVGLAASVMFLPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
SEVGQAVGDYQNHPLTKGILEGYNTMDTLASLMFGMLIIDILRKKGIDQPSAQTKYLIRA
HHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
AFIAAAGLAFVYVSLFFLGATAGDIAIGAKNGGEILTNYVTREFGDLGSVLLSSVVTLAC
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTTAVGLVSACSEFFNELMPKLSYRFLVIFLSVICAVIANVGLTQLIAISIPVLMTIYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
AIALVVITFLTAYFANPQFAHRAVLSVALFFGIFDGLKVAASSLTGADGQAVNSLAQGFI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
HVVDVMSPVLSKLPLYEEGMAWLLPTIATIVLCLVFGRGRDQTAAA
HHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]