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The map label for this gene is rimK1

Identifier: 126172754

GI number: 126172754

Start: 571254

End: 572159

Strand: Direct

Name: rimK1

Synonym: Sbal_0505

Alternate gene names: 126172754

Gene position: 571254-572159 (Clockwise)

Preceding gene: 126172753

Following gene: 126172759

Centisome position: 11.14

GC content: 47.79

Gene sequence:

>906_bases
ATGAAAATAGGTATTTTATCCCAATTCCCCCAGTTGTATTCGACACAACGTTTAGTGGAGGCTTGTCAAAGCCGCGGACA
TGAAGCGGTTGTGATCAATACACTGAACTGCTACATGAATATTAATTCCATTAAGCCTAGCATTCATTATGAAGGCACTG
AATTAGTTGGCTTTGATGCCATCATTCCGCGTATTCATGCCAGCGTGACTTTTTATGGCTGTGCCGTTGTTCGTCAGTTC
GAAATGATGGGCGTGTATGCGGCGAATGATTCAATTTCCATTGCGCGTTCTCGGGACAAATTACGGGCGCTGCAATTGCT
CTCCCGCAAAGGGATTGGTATGCCTGTGACAGGCTTTGCCAATAAGCCAAATGATATCCCCGACCTCATCAATATGGTGG
GCGGCGCTCCGTTAGTCATTAAGTTACTCGAAGGTACTCAAGGGATTGGCGTGGTATTAGCTGAGACGAAAACGGCAGCT
GAGAGCGTGATAGAAGCATTCTTGGGGCTGAAAGCGAATATCTTAGTGCAGGAATACATTAAAGAATCCAATGGCAGTGA
TATTCGTTGTTTTGTTGTCGGTGACAAGGTCATCGCCTCTATGAAGCGCCAAGGACCCGAGGGTGATTTTCGCTCCAATC
TGCATTTGGGCGGCTGTGGTGAGGTGGTTAAAATCACTGCTGTGGAACGTAAGATGGCGATTGCGGCGGTGAAAGCGATG
GGATTGGTGGTCGCTGGGGTGGATATTTTACGCTCTAATCGTGGCCCTTTGATCCTTGAGGTGAATTCGGCGCCAGGCAT
TGAAGGGATTGAGCAAACCACAGGGATTTCGGTGACTGAGCCCATTGTTGAATATATCGAGAAAATGGTCGCAGCCCGTA
AAACCAACAGGCCCATCATTGCCTAA

Upstream 100 bases:

>100_bases
TTACACTGAGCAATAGACAAATATCAGCCCCTTTTCTGTCGCTGTGCTTTTAGTGCCGCAGAATGGTTTATTCACTCATG
CTTAAGTTGGTCACTCAGCA

Downstream 100 bases:

>100_bases
TGGTTGATTGTGCTAAAACAACGCCACCGTTTAAGGTGGCGTTTTTTTATGCTTAGCCCTTAGCCCTTAGCCCTTAGCCC
TTAGACGTAAGACTCTAAAT

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIGILSQFPQLYSTQRLVEACQSRGHEAVVINTLNCYMNINSIKPSIHYEGTELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVYAANDSISIARSRDKLRALQLLSRKGIGMPVTGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVIASMKRQGPEGDFRSNLHLGGCGEVVKITAVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA

Sequences:

>Translated_301_residues
MKIGILSQFPQLYSTQRLVEACQSRGHEAVVINTLNCYMNINSIKPSIHYEGTELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVYAANDSISIARSRDKLRALQLLSRKGIGMPVTGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVIASMKRQGPEGDFRSNLHLGGCGEVVKITAVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA
>Mature_301_residues
MKIGILSQFPQLYSTQRLVEACQSRGHEAVVINTLNCYMNINSIKPSIHYEGTELVGFDAIIPRIHASVTFYGCAVVRQF
EMMGVYAANDSISIARSRDKLRALQLLSRKGIGMPVTGFANKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAA
ESVIEAFLGLKANILVQEYIKESNGSDIRCFVVGDKVIASMKRQGPEGDFRSNLHLGGCGEVVKITAVERKMAIAAVKAM
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPIIA

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=226, Percent_Identity=30.0884955752212, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI134254462, Length=225, Percent_Identity=27.5555555555556, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787076, Length=288, Percent_Identity=61.8055555555556, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK1_SHEB5 (A3CZX1)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001048903.1
- STRING:   A3CZX1
- GeneID:   4841504
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_0505
- NMPDR:   fig|325240.3.peg.1670
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   INVAQEP
- PhylomeDB:   A3CZX1
- ProtClustDB:   CLSK905802
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32423; Mature: 32423

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGILSQFPQLYSTQRLVEACQSRGHEAVVINTLNCYMNINSIKPSIHYEGTELVGFDA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEECCCEEEEHHH
IIPRIHASVTFYGCAVVRQFEMMGVYAANDSISIARSRDKLRALQLLSRKGIGMPVTGFA
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVIASMKRQGPEGDFRSNLHLGGCGEVVKITAVERKMAIAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPII
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
A
C
>Mature Secondary Structure
MKIGILSQFPQLYSTQRLVEACQSRGHEAVVINTLNCYMNINSIKPSIHYEGTELVGFDA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCCCCCEEECCCEEEEHHH
IIPRIHASVTFYGCAVVRQFEMMGVYAANDSISIARSRDKLRALQLLSRKGIGMPVTGFA
HHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
NKPNDIPDLINMVGGAPLVIKLLEGTQGIGVVLAETKTAAESVIEAFLGLKANILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHH
KESNGSDIRCFVVGDKVIASMKRQGPEGDFRSNLHLGGCGEVVKITAVERKMAIAAVKAM
HCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
GLVVAGVDILRSNRGPLILEVNSAPGIEGIEQTTGISVTEPIVEYIEKMVAARKTNRPII
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA